本文關(guān)鍵詞： 聚乳酸 血液透析膜 親水性 透析性能 血液相容性　出處：《青島科技大學》2017年碩士論文　論文類型：學位論文

【摘要】：聚乳酸(PLA)作為一種生物基聚合物,具有良好的成膜性、可控降解、結(jié)構(gòu)易調(diào)控以及附加值高等優(yōu)點。但是PLA微孔膜的表面抗污性以及生物相容性需要進一步提高,以滿足其在生物醫(yī)用領(lǐng)域如透析方面的潛在應用。本課題主要采用非溶劑誘導相分離法制備PLA微孔膜,通過表面微溶脹及界面交聯(lián)的方式在膜表面引入親水性鏈段(如PVP鏈段),提高其親水抗污性能,并進一步通過表面修飾抗凝基團(如肝素、水蛭素以及類肝素),從而提高PLA微孔膜的血液相容性。主要研究內(nèi)容如下:肝素固定聚乳酸膜的制備及其血液相容性研究;水蛭素氫鍵固定聚乳酸膜的制備及抗凝血性研究;類肝素修飾聚乳酸微孔膜的血液相容性研究。通過對聚乳酸微孔膜的物理和化學形態(tài)結(jié)構(gòu)、親水性、血液相容性以及透析性能等方面進行研究,結(jié)論如下:(1)通過酰胺化反應及吸附將肝素固定到PLA膜表面,提高了PLA膜表面的血液相容性。首先通過酰胺化反應合成Hep-APTES化合物,然后將Hep-APTES通過自縮聚反應以及多層吸附等方式固定到PLA膜表面,從而制備出肝素化修飾PLA膜。實驗結(jié)果表明肝素固定后的PLA膜具有良好的血液相容性。(2)天然水蛭素可以和PVP形成氫鍵。通過氫鍵相互作用將水蛭素分子固定在聚乳酸膜表面,從而制備出一種新型的抗凝聚乳酸膜。凝血四項(APTT,PT,TT,FIB)測試結(jié)果表明水蛭素固定膜具有良好的血液相容性。透析模擬實驗表明,新制備的水蛭素固定聚乳酸膜具有良好的尿素、肌酐、溶菌酶清除率和較高的BSA保留率。(3)通過自由基共聚合成類肝素預聚物P(SSNa-VTES-AAH),然后利用表面微溶脹及界面交聯(lián)策略,將類肝素共聚物P(SSNa-VTES-AAH)固定在膜表面,從而提高聚乳酸膜的血液相容性。類肝素分子修飾的PLA膜表面血液相容性明顯提高,血小板吸附量相對較少,并且具有良好的透析性能。
[Abstract]:Polylactic acid (PLA), as a biological polymer, has the advantages of good film-forming, controllable degradation, easy structure control and high added value. However, the surface antifouling and biocompatibility of PLA microporous membranes need to be further improved. In order to meet the potential applications in biomedical applications such as dialysis, PLA microporous membranes were prepared by non-solvent induced phase separation. Hydrophilic segments (such as PVP segment) were introduced into the membrane surface by micro-swelling and interfacial crosslinking to improve their hydrophilic and antifouling properties, and further surface modification of anticoagulant groups (such as heparin, heparin, heparin) was carried out to improve the hydrophilic and antifouling properties of the membranes. Hirudin and heparin can improve the blood compatibility of PLA microporous membrane. The main research contents are as follows: preparation and blood compatibility of PLA membrane fixed with heparin, preparation of hirudin hydrogen bond fixed membrane and anticoagulant study; The study on the blood compatibility of poly (lactic acid) microporous membrane modified by heparin. The physical and chemical morphology, hydrophilicity, blood compatibility and dialysis performance of the membrane were studied. Conclusion: (1) Heparin was immobilized on the surface of PLA membrane by amidation reaction and adsorption, which improved the blood compatibility of PLA membrane surface. Firstly, Hep-APTES compounds were synthesized by amidation reaction. Then Hep-APTES was immobilized on the surface of PLA film by self-condensation reaction and multilayer adsorption. Heparinized modified PLA membrane was prepared. The experimental results showed that the heparin immobilized PLA membrane had good blood compatibility. 2) Natural hirudin could form hydrogen bond with PVP. Hirudin molecules were immobilized on the surface of polylactic acid membrane by hydrogen bond interaction. Thus a new anticoagulant polylactic acid membrane was prepared. The test results showed that hirudin fixed membrane had good blood compatibility, and the dialysis simulation experiment showed that the newly prepared hirudin immobilized PLA membrane had good urea. Creatinine, lysozyme clearance and high retention rate of BSA were synthesized by free radical copolymerization of heparin-like prepolymer (Pna-VTES-AAHN), and then the heparin-like copolymer PSSNa-VTES-AAHH was immobilized on the membrane surface by surface microswelling and interfacial crosslinking strategy. Therefore, the blood compatibility of polylactic acid membrane was improved, the surface of PLA membrane modified by heparin was improved obviously, the platelet adsorption was relatively small, and the hemodialysis performance was good.
【學位授予單位】：青島科技大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：TQ317;R318.08

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相關(guān)期刊論文 前3條

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本文編號：1543916