本文關(guān)鍵詞： 縱條紋炭角菌 化學(xué)成分 改善睡眠 抗線蟲 抗腫瘤　出處：《西南科技大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：縱條紋炭角菌(Xylaria striata Pat.1887)屬于子囊菌門炭角菌屬炭角菌科真菌,主要生長于腐朽樹皮或活樹根上。除本課題組對(duì)縱條紋炭角菌的長期深入研究外,尚無該菌的相關(guān)報(bào)道。活性測(cè)試顯示,該菌子實(shí)體粉末是無毒的,且具有良好的改善睡眠作用。為闡明該菌子實(shí)體改善睡眠作用的活性成分,本論文以人工培育的縱條紋炭角菌子實(shí)體為原料,以戊巴比妥鈉誘導(dǎo)小鼠睡眠實(shí)驗(yàn)為活性指導(dǎo),對(duì)其化學(xué)成分進(jìn)行深入研究。綜合利用多種柱色譜技術(shù)對(duì)該菌子實(shí)體的乙醇提取物進(jìn)行了分離純化,共分離得到13個(gè)化合物,其中包括2個(gè)新化合物和11個(gè)已化合物。將得到的部分化合物進(jìn)行了改善睡眠、抗腫瘤和抗線蟲研究,研究結(jié)果為該菌在改善睡眠、抗腫瘤和抗線蟲方面的應(yīng)用提供理論依據(jù)。本論文主要包括以下三個(gè)方面的工作:一、縱條紋炭角菌子實(shí)體的安全性和改善睡眠活性成分研究。通過急性毒性試驗(yàn)對(duì)其安全性進(jìn)行評(píng)價(jià),結(jié)果表明口服2000 mg/kg子實(shí)體粉末的小鼠,各項(xiàng)生理指標(biāo)正常且內(nèi)臟組織切片無病變跡象。以睡眠活性(戊巴比妥鈉誘導(dǎo)睡眠實(shí)驗(yàn))為指導(dǎo),對(duì)該菌改善睡眠的活性成分進(jìn)行了追蹤,結(jié)果表明活性部位為氯仿萃取物,改善睡眠活性成分為氯仿萃取物中的xylastriamide A和麥角甾醇。二、縱條紋炭角菌子實(shí)體的乙醇提取物中化學(xué)成分研究。首次對(duì)縱條紋炭角菌子實(shí)體的化學(xué)成分進(jìn)行了系統(tǒng)研究,綜合利用重結(jié)晶和多種色譜技術(shù),從縱條紋炭角菌子實(shí)體乙醇提取物中分離得到13個(gè)化合物,利用波譜技術(shù)(ESI-MS和NMR)分別鑒定為:xylastriamide A(1)、xylastriasan A(2)、細(xì)胞松弛素D(3)、19,20-環(huán)氧細(xì)胞松弛素Q(4)、細(xì)胞松弛素Q(5)、19,20-環(huán)氧細(xì)胞松弛素D(6)、19,20-環(huán)氧細(xì)胞松弛素C(7)、反式對(duì)羥基肉桂酸乙酯(8)、吲哚-3-甲醛(9)、次黃嘌呤核苷(10)、尿嘧啶(11)、尿嘧啶核苷(12)、麥角甾醇(13)�；衔�1-12均首次從該菌子實(shí)體中分離得到,其中化合物1和2是新化合物。三、化學(xué)成分的改善睡眠、抗腫瘤和抗線蟲活性研究。通過戊巴比妥鈉誘導(dǎo)小鼠睡眠實(shí)驗(yàn)對(duì)化合物1~7進(jìn)行了改善睡眠測(cè)試,結(jié)果顯示化合物1對(duì)小鼠睡眠有顯著改善作用。采用MTT法對(duì)化合物1~5和9進(jìn)行體外腫瘤細(xì)胞毒活性初篩,實(shí)驗(yàn)結(jié)果顯示化合物1和9(分別為100和40μmol/L)對(duì)HCT116、B16、U937腫瘤細(xì)胞的相對(duì)抑制率均大于50%。采用殺線活性實(shí)驗(yàn),對(duì)化合物1~7進(jìn)行了抗線蟲初步篩選,結(jié)果表明化合物1具有一定的抗線蟲活性,其IC50值為103.54μg/mL。
[Abstract]:Xylaria striata Pat.1887) belongs to Ascomycetes. It is mainly grown on decayed bark or living tree root. Except for the long-term in-depth study of this fungus, there is no related report of this bacterium. The activity test shows that the powder of fruiting body of this fungus is nontoxic. In order to elucidate the active components of the fruiting body to improve sleep, the artificial cultivated fruiting body of C. longitudinalis was used as the raw material. Under the guidance of pentobarbital sodium induced sleep test in mice, the chemical constituents were studied. The ethanol extract from the fruiting body of the fungus was separated and purified by using a variety of column chromatography techniques. A total of 13 compounds were isolated, including 2 new compounds and 11 hexane compounds. Some of the compounds were studied to improve sleep, anti-tumor and anti-nematode. The results provide theoretical basis for the application of the bacteria in improving sleep, anti-tumor and anti-nematode. This paper mainly includes the following three aspects of work: 1. The study on the safety of fruiting bodies and the improvement of sleep active components of C. longitudinalis. The safety was evaluated by acute toxicity test. The results showed that the mice treated with 2 000 mg/kg fruiting body powder were given orally. Under the guidance of sleep activity (pentobarbital sodium induced sleep experiment), the active components of the bacteria to improve sleep were tracked. The results showed that the active fraction was chloroform extract, and the active components for improving sleep were xylastriamide A and ergosterol in chloroform extract. Studies on the chemical constituents of the ethanol extracts from the fruiting bodies of C. longitudinalis. The chemical constituents of the fruiting bodies of C. longitudinalis were studied systematically for the first time, and the recrystallization and various chromatographic techniques were used comprehensively. Thirteen compounds were isolated from the ethanol extract from the fruiting body of C. longitudinalis. The compounds were identified as 1: xylastriamide Agna 1 by spectroscopic techniques (ESI-MS and NMRs), respectively. Xylastriasan A ~ (2 +), cytochalasin D _ (3) ~ (1) ~ (19) ~ (20) -epoxy-cytochalasin Q _ (4), cytochalasin (Q _ (5)) ~ (5) ~ (19). 20-epoxy-cytochalasin D _ (6) / ~ (19) -epoxy-cytochalasin C _ (7), trans-para-hydroxycinnamate ethyl ester ~ (8), indole-3-formalin ~ (9), Hypoxanthine nucleoside 10 (~ (10)). Compounds 1-12 were isolated from the fruiting body of this bacterium for the first time. Compounds 1 and 2 are new compounds. Studies on the effects of chemical components on sleep, anti-tumor and anti-nematode activity. The sleep test of compound 1H7 was carried out in mice induced by pentobarbital sodium. The results showed that compound 1 could significantly improve the sleep of mice. The cytotoxic activity of compounds 1 5 and 9 was screened by MTT method in vitro. The experimental results showed that compounds 1 and 9 (100 and 40 渭 mol / L, respectively) were effective on HCT 116 B16. The relative inhibition rates of U937 tumor cells were higher than 50. The preliminary screening of compound 1H7 against nematodes showed that compound 1 had a certain activity against nematodes. Its IC50 value is 103.54 渭 g / mL.
【學(xué)位授予單位】：西南科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R284;R285

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本文編號(hào)：1471480