本文關(guān)鍵詞： 量子點 表面配體 發(fā)光性質(zhì) 腸道病毒EV71 雙模式免疫傳感器　出處：《武漢大學》2017年碩士論文　論文類型：學位論文

【摘要】：納米材料具有巨大的科研價值和廣闊的應(yīng)用前景。在眾多納米材料中,量子點和磁性納米材料因其獨特的性質(zhì)而成為最具研究價值的納米材料。眾所周知,量子點的性質(zhì)主要由其晶體結(jié)構(gòu)、表面配體及所處環(huán)境決定。利用表面配體調(diào)控量子點發(fā)光性質(zhì)具有靈活性多樣性等優(yōu)勢。磁性納米材料具有優(yōu)良的磁性及易操控性,已被廣泛用于生物醫(yī)學分析化學檢測中�；诖�,本論文開展了以下工作:(1)將磁性納米材料與酶放大策略、比色分析法、電化學免疫分析技術(shù)相結(jié)合,構(gòu)建了基于雙標記磁性納米探針放大策略的比色和電化學雙模式免疫傳感器,并將其應(yīng)用于現(xiàn)場檢測腸道病毒EV71。整個實驗可以在2 h內(nèi)完成,比色分析和電化學檢測的檢出限分別達到1.0 ng/mL和10.0 pg/mL。該方法靈敏、簡便,選擇性好,抗干擾能力強,無需樣品預處理和復雜昂貴的儀器及試劑,而且樣品用量少至20μL。臨床樣品檢測結(jié)果表明該方法的檢測結(jié)果與RT-PCR結(jié)果相一致。(2)合成了以油胺/油酸為表面配體的硒化鎘量子點(CdSe QDs),研究了辛硫醇取代中表面配體對其光致發(fā)光以及電致化學發(fā)光性質(zhì)的影響。結(jié)果表明,辛硫醇能夠強烈地猝滅CdSe QDs的帶邊發(fā)光;隨著表面辛硫醇含量的增加,CdSe QDs的電致化學發(fā)光強度卻逐漸增大,而電致化學發(fā)光光譜峰位置沒有顯著影響。據(jù)此提出了CdSe QDs的發(fā)光模型,揭示了表面配體對量子點發(fā)光機理的影響:CdSe QDs不僅存在與本征發(fā)射相關(guān)的激子態(tài),還存在與表面密切相關(guān)的深能級陷阱態(tài)以及由內(nèi)部雜質(zhì)引起的淺能級陷阱態(tài)。
[Abstract]:Among many nanomaterials, quantum dots and magnetic nanomaterials have become the most valuable nanomaterials due to their unique properties. The properties of quantum dots are mainly due to their crystal structure. The surface ligands and their environment are determined. The use of surface ligands to regulate the luminescent properties of quantum dots has the advantages of flexibility and diversity. The magnetic nanomaterials have excellent magnetic properties and easy to control. Has been widely used in biomedical analytical chemistry detection. Based on this, this paper carried out the following work: 1) combining magnetic nanomaterials with enzyme amplification strategy, colorimetric analysis, electrochemical immunoassay. A colorimetric and electrochemical dual-mode immunosensor based on double-label magnetic nanoprobe amplification strategy was constructed and applied to the detection of enterovirus EV71.The whole experiment could be completed in 2 hours. The detection limits of colorimetric analysis and electrochemical detection were 1.0 ng/mL and 10.0 PG / mL, respectively. The method was sensitive, simple, selective and anti-jamming. There is no need for sample pretreatment and complex and expensive instruments and reagents. In addition, the dosage of the sample is as low as 20 渭 L. the results of clinical sample detection show that the results of this method are in agreement with the results of RT-PCR. 2) the cadmium selenide quantum dots with oleic acid / oleic acid as the surface ligands were synthesized. CdSe QDs. The effects of the ligands on the photoluminescence and electrochemiluminescence properties of CdSe QDs were studied. The results showed that octyl mercaptan could quench the band edge luminescence of CdSe QDs. The electrochemiluminescence intensity of CdSe QDs increases with the increase of octyl mercaptan content. However, the location of the electrochemiluminescence peak was not significantly affected. Based on this, the luminescence model of CdSe QDs was proposed. The effect of surface ligands on the luminescence mechanism of quantum dots is revealed. The exon states related to intrinsic emission are not only present in the QDs of: CdSe. There are also deep level trap states closely related to the surface and shallow level trap states caused by internal impurities.
【學位授予單位】：武漢大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R446.6;O657

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