天堂国产午夜亚洲专区-少妇人妻综合久久蜜臀-国产成人户外露出视频在线-国产91传媒一区二区三区

無(wú)功能型垂體腺瘤的侵襲性相關(guān)蛋白組學(xué)初步篩選研究

發(fā)布時(shí)間:2018-01-15 11:50

  本文關(guān)鍵詞:無(wú)功能型垂體腺瘤的侵襲性相關(guān)蛋白組學(xué)初步篩選研究 出處:《新疆醫(yī)科大學(xué)》2017年碩士論文 論文類(lèi)型:學(xué)位論文


  更多相關(guān)文章: 無(wú)功能型垂體腺瘤 侵襲性 蛋白組學(xué) 差異表達(dá)蛋白 雙向凝膠電泳 基質(zhì)輔助激光解析電離飛行時(shí)間質(zhì)譜


【摘要】:目的:我們擬尋找與無(wú)功能型垂體腺瘤的侵襲性相關(guān)的重要蛋白,為全面、系統(tǒng)研究無(wú)功能型垂體腺瘤侵襲生長(zhǎng)的機(jī)制提供理論依據(jù),為今后利用蛋白質(zhì)組學(xué)方法全面、系統(tǒng)研究無(wú)功能型垂體腺瘤的病因,以及分子靶向治療、基因治療提供新的突破點(diǎn)。方法:收集2015年10月至2016年10月期間新疆維吾爾自治區(qū)人民醫(yī)院神經(jīng)外科收治的經(jīng)本院手術(shù)治療的18例無(wú)功能型垂體瘤病人的腫瘤標(biāo)本(侵襲性生長(zhǎng)9例,非侵襲性生長(zhǎng)9例)。用二維凝膠電泳結(jié)合質(zhì)譜技術(shù)尋找在無(wú)功能型垂體腺瘤中表達(dá)的差異性蛋白;應(yīng)用雙向凝膠電泳(2-DE)結(jié)合基質(zhì)輔助激光解析電離飛行時(shí)間質(zhì)譜技術(shù)(MALDI-TOF-MS)對(duì)侵襲性無(wú)功能型垂體腺瘤及非侵襲性無(wú)功能型垂體腺瘤腫瘤組織進(jìn)行蛋白質(zhì)翻譯水平的篩選,并比較組織蛋白的異同,尋找與無(wú)功能型垂體腺瘤侵襲性相關(guān)的蛋白質(zhì)及可能在臨床診斷中應(yīng)用的蛋白質(zhì)。結(jié)果:(1)通過(guò)雙向電泳結(jié)果(通過(guò)PDQuest軟件顯不同組的差異表達(dá)蛋白質(zhì)點(diǎn)),建立了無(wú)功能型垂體腺瘤的的2-DE圖譜。(2)通過(guò)研究我們找到4種差異蛋白點(diǎn),其中p14ARF蛋白在侵襲性無(wú)功能型垂體腺瘤的腫瘤組織中較非侵襲性無(wú)功能型垂體腺瘤的腫瘤表達(dá)下調(diào),EGFR、HMGA2、PIK3CA蛋白在侵襲性無(wú)功能型垂體腺瘤的腫瘤組織中較非侵襲性無(wú)功能型垂體腺瘤的腫瘤表達(dá)上調(diào)。結(jié)論:這4種差異蛋白可能與無(wú)功能型垂體腺瘤的侵襲性生長(zhǎng)有關(guān)。
[Abstract]:Objective: to search for important proteins related to invasiveness of non-functional pituitary adenomas, and to provide theoretical basis for a comprehensive and systematic study of the mechanism of invasiveness and growth of non-functional pituitary adenomas. In order to study the etiology and molecular targeted therapy of non-functional pituitary adenoma systematically by proteomics. Gene therapy provides a new breakthrough. Methods:. From October 2015 to October 2016, 18 patients with disfunctional pituitary adenoma treated in neurosurgery department of Xinjiang Uygur Autonomous region people's Hospital were collected (. Invasive growth was found in 9 cases. Two dimensional gel electrophoresis and mass spectrometry were used to search for differentially expressed proteins in non-functional pituitary adenomas. Two-dimensional gel electrophoresis (2-DE) combined with matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MSM). The protein translation level of invasive and non-invasive pituitary adenomas was screened. The differences and similarities of histone were compared. Search for proteins associated with invasiveness of nonfunctional pituitary adenomas and proteins that may be used in clinical diagnosis. PDQuest software was used to show the differentially expressed protein spots in different groups. The 2-DE map of non-functional pituitary adenoma was established. The expression of p14ARF protein was down-regulated in tumor tissues of invasive non-functional pituitary adenomas than that of non-invasive pituitary adenomas. The expression of PIK3CA protein was up-regulated in invasive non-functional pituitary adenomas than in non-invasive pituitary adenomas. Conclusion:. These four differentially expressed proteins may be related to the invasive growth of nonfunctional pituitary adenomas.
【學(xué)位授予單位】:新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】:碩士
【學(xué)位授予年份】:2017
【分類(lèi)號(hào)】:R736.4

【參考文獻(xiàn)】

相關(guān)期刊論文 前7條

1 楊建鋒;金杭斌;謝璐;顧偉剛;沈紅璋;張?bào)泺P;;應(yīng)用蛋白組學(xué)技術(shù)篩選肝外膽管癌膽汁腫瘤標(biāo)志物的初步研究[J];中華消化內(nèi)鏡雜志;2016年11期

2 顧小燕;吳昌平;金建華;蔣敬庭;;胰腺癌早期診斷的研究現(xiàn)狀及進(jìn)展[J];中國(guó)醫(yī)藥生物技術(shù);2016年02期

3 王杰;衣素琴;王歡;周s,

本文編號(hào):1428212


資料下載
論文發(fā)表

本文鏈接:http://sikaile.net/shoufeilunwen/mpalunwen/1428212.html


Copyright(c)文論論文網(wǎng)All Rights Reserved | 網(wǎng)站地圖 |

版權(quán)申明:資料由用戶(hù)35128***提供,本站僅收錄摘要或目錄,作者需要?jiǎng)h除請(qǐng)E-mail郵箱bigeng88@qq.com
欧美在线观看视频三区| 黄色激情视频中文字幕| 亚洲第一区二区三区女厕偷拍| 国产av一区二区三区麻豆| 青青操日老女人的穴穴| 高潮少妇高潮久久精品99| 国产欧美日韩在线一区二区| 青青操成人免费在线视频| 国内尹人香蕉综合在线| 久久青青草原中文字幕| 日韩av欧美中文字幕| 日韩成人高清免费在线| 国产一级二级三级观看| 中文字幕精品少妇人妻| 国产精品一区二区成人在线| 一区二区三区免费公开| 富婆又大又白又丰满又紧又硬| 日韩一区二区免费在线观看| 亚洲一区二区三区国产| 日韩精品你懂的在线观看| 国内外激情免费在线视频| 亚洲国产成人精品福利| 国产高清一区二区不卡| 国产成人精品一区二三区在线观看| 国产高清一区二区白浆| 欧美一区二区三区不卡高清视| 麻豆印象传媒在线观看| 久久碰国产一区二区三区| 免费观看成人免费视频| 欧美日韩综合在线第一页| 国产欧美日韩精品自拍| 风间中文字幕亚洲一区| 深夜视频在线观看免费你懂| 九九九热视频免费观看| 欧洲自拍偷拍一区二区| 久久福利视频这里有精品| 国产精品成人一区二区三区夜夜夜 | 一区二区三区日本高清| 日本午夜精品视频在线观看| 精品视频一区二区不卡| 自拍偷拍福利视频在线观看|