本文關(guān)鍵詞：藥用真菌桑黃化學(xué)成分的研究　出處：《安徽醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 桑黃 化學(xué)成分 提取分離 高通量篩選 抗腫瘤活性

【摘要】：藥用真菌火木層孔菌Phellinus igniarius(L.Ex Fr.)屬于擔(dān)子菌門(mén)、層菌綱、非褶菌目、多孔菌科、針層孔菌屬,是我國(guó)傳統(tǒng)中藥桑黃的重要來(lái)源菌之一。桑黃又名桑耳、桑臣、樹(shù)雞、桑黃菇、桑上寄生、猢猻眼等,素有“森林黃金”之美稱。桑黃主要分布于我國(guó)西北、東北、華北及四川和云南等地,在日本、韓國(guó)和朝鮮等東亞國(guó)家亦有分布。主要寄生于桑樹(shù)、楊樹(shù)、櫟樹(shù)等闊葉樹(shù)腐朽的樹(shù)干上。子實(shí)體入藥,具有廣泛的藥用價(jià)值。在國(guó)際上被公認(rèn)為目前在生物治癌領(lǐng)域中有效率最高的藥用真菌。在傳統(tǒng)中藥中,桑黃主要用于抗腫瘤、保護(hù)肝臟、治療胃潰瘍等。研究表明桑黃中含有多種有效成分,已報(bào)道分離到的化學(xué)成分主要包括萜類、甾體類、黃酮類、吡喃酮類、酚性化合物、香豆素類、多糖等種類的化合物;藥理作用主要為免疫調(diào)節(jié)、抗腫瘤、抗氧化、保護(hù)肝臟和抗肝硬化等。為了探索桑黃抗腫瘤作用的物質(zhì)基礎(chǔ),便于深入開(kāi)發(fā)我國(guó)珍貴的桑黃資源,本文分別對(duì)野生桑黃和人工種植桑黃95%乙醇提取物進(jìn)行了系統(tǒng)的化學(xué)成分研究。本研究先后利用HPD 100大孔樹(shù)脂、MCI、ODS、C8、制備硅膠薄層層析、硅膠柱層析、凝膠柱層析等多種層析技術(shù)對(duì)桑黃中的化學(xué)成分進(jìn)行分離純化,運(yùn)用核磁共振、質(zhì)譜等有機(jī)波譜解析技術(shù)對(duì)所分離到的單體化合物進(jìn)行結(jié)構(gòu)鑒定,從桑黃中一共分離鑒定了25個(gè)化合物。其中,從野生桑黃中分離鑒定了12個(gè)化合物,分別為:尿嘧啶(1)、乙基-β-D-葡萄糖苷(2)、海藻糖(3)、呋喃(4)、尿嘧啶核苷(5)、丙三醇(6)、腦苷脂B(7)、澤瀉醇(8)、(22E,24R)-麥角甾-7,22-二烯-3β,5α,6β-三醇(9)、鄰苯二甲酸二甲酯(10)、櫻花亭(11)、(22E,24R)-麥角甾-7,9(11),22-三烯-3β,5α,6α-三醇(12)。從人工種植桑黃中分離得到13個(gè)化合物,分別為:豆甾-7,22-二烯-3β,5α,6α-三醇(13)、對(duì)乙酰氨基酚(14)、4-(3,4-二羥苯基)-3-丁烯-2-酮(15)、原兒茶醛(16)、3α-羥基木栓烷-2-酮(17)、3-羥基木栓烷-3-烯-2-酮(18)、麥角甾-4,6,8(14),22-四烯-3-酮(19)、麥角甾醇過(guò)氧化物(20)、尿嘧啶(1)、尿嘧啶核苷(5)、Inotilone(21)、Inoseavin A(22)、Phellibaumin E(23)。其中乙基-β-D-葡萄糖苷(2)、海藻糖(3)、呋喃(4)、澤瀉醇(8)、鄰苯二甲酸二甲酯(10)、對(duì)乙酰氨基酚(14)六個(gè)化合物為首次從桑黃所在的針層孔菌屬(Phellinus Quel.)中發(fā)現(xiàn);其中(22E,24R)-麥角甾-7,22-二烯-3β,5α,6β-三醇(9)、(22E,24R)-麥角甾-7,9(11),22-三烯-3β,5α,6α-三醇(12)兩個(gè)化合物為首次從桑黃(Phellinus igniarius)中發(fā)現(xiàn)。本課題還選取了41株離體腫瘤細(xì)胞和2株正常倉(cāng)鼠細(xì)胞,采用Cell Titer-GLo ATP熒光活性檢測(cè)法對(duì)其中的11個(gè)化合物(15-23,1,5)進(jìn)行了高通量篩選,發(fā)現(xiàn)4個(gè)體外抗腫瘤效果明顯的化合物(15,18,19,20),并且結(jié)果顯示所有化合物對(duì)正常細(xì)胞均無(wú)毒副作用。
[Abstract]:The medicinal fungus Phellinus igniarius(L.Ex Fr. belongs to basidiomycetes, lamella, non-pleiophyllum, polyporaceae and Needles. Mulberry yellow also known as mulberry ear, Sanchen, tree chicken, mulberry mushroom, mulberry parasitism, monkey eyes, etc., known as "forest gold". Mulberry yellow is mainly distributed in the northwest of China. Northeast China, North China, Sichuan and Yunnan, also distributed in East Asian countries such as Japan, South Korea and Korea. Parasitic mainly on the rotten trunks of mulberry, poplar, oak and other broad-leaved trees. The fruiting body is used for medicine. It has a wide range of medicinal value. It is recognized as the most effective medicinal fungus in the field of biological cancer treatment. In traditional Chinese medicine, mulberry is mainly used for anti-tumor and liver protection. Treatment of gastric ulcer. Studies show that Mulberry yellow contains a variety of active components, the reported chemical constituents mainly include terpenoids, steroids, flavonoids, pyrone, phenolic compounds, coumarins. Polysaccharides and other kinds of compounds; The pharmacological effects are mainly immune regulation, anti-tumor, anti-oxidation, protection of liver and anti-cirrhosis, etc. In order to explore the material basis of anti-tumor effect of mulberry yellow, it is convenient to further develop the precious mulberry yellow resources in China. The chemical constituents of ethanol extracts from wild and artificial mulberry yellow 95% were studied in this paper. Preparation of silica gel thin-layer chromatography, silica gel column chromatography, gel column chromatography and other chromatography techniques to separate and purify the chemical components in Morus Mori, and use nuclear magnetic resonance (NMR). The structures of the isolated monomers were identified by mass spectrometry and other organic spectral analysis techniques. 25 compounds were isolated and identified from mulberry and 12 compounds were isolated and identified from wild mulberry. The results were as follows: uracil, ethyl- 尾 -glucoside, trehalose, furan, uracil, triglyceride, glucoside B7, alismatine 8). The results were as follows: (1) 2E ~ (22) E ~ (24) R ~ (+) -ergosterol -7 ~ (22) -diene -3 尾 -5 偽 ~ (6) 尾 -triol ~ (9), 10 ~ (10) dimethyl phthalate, and 11 ~ (11) ~ (22) E of Cherry Blossom Pavilion. A total of 13 compounds were isolated from cultivated mulberry, which were identified as: Dousta-7. The results were as follows: (1) ~ (2) ~ (24) R ~ (2 +) -ergoster-7 ~ (9) C ~ (2 +) ~ (22) -triene-3 尾 -5 偽 -triol 6 偽 -triol ~ (12). 22-diene--3 尾 -5 偽 -butene-6 偽 -triol 13C, paracetamol 14, 4-dihydroxyphenyl 3-butene-3-butene-15, protocatechuic aldehydes 16). 3 偽 -hydroxyhydrin -2-one (17) -3-hydroxy-3-ene-2-one (18G), ergosterol -4 (6) (8An), 2-triene-3-one (19C), and (3) 偽 -hydroxy-thromboxygene-2-ketonone (3-hydroxy-trimethylethylene-3-ketene). Ergosterol peroxides 20, uracil 1, uracil 5, Inotiloneus 21, Inoseavin Agn22). Phellibaumin 23. Ethyl- 尾 -Dglucoside 2, trehalose 3, furan 4, alisin 8, dimethyl phthalate 10). Six compounds were first found from Phellinus Quel.in Mulberry. Among them, 22E ~ (22) E ~ (24) R ~ (+) -ergosterol -7 ~ (22) -diene ~ (-3) -5 偽 ~ (6) 尾 -triol ~ (9) ~ (-) ~ (22) E ~ (2 +) ~ (24) R ~ (-) -ergoster-7 ~ (7) -9 ~ (11) C ~ (22) -triene ~. 5 偽. Two compounds from Phellinus igniarius were isolated from Phellinus japonicus for the first time. 41 isolated tumor cells and 2 normal hamster cells were selected. Cell Titer-GLo ATP fluorescence assay was used to screen 11 of them. Four compounds with obvious antitumor effect in vitro were found. The results showed that all the compounds had no toxic and side effects on normal cells.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R284.1

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本文編號(hào)：1426000