本文關(guān)鍵詞：BTBD7及E-cadherin在唾液腺腺樣囊性癌中的表達(dá)與相關(guān)性的研究　出處：《山東大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： BTBD7 E-cadherin 唾液腺 腺樣囊性癌 免疫組織化學(xué)

【摘要】：目的:檢測(cè)BTBD7和E-cadherin在唾液腺腺樣囊性癌(SACC)中的表達(dá)情況,并研究其與SACC不同臨床病理特性的相關(guān)關(guān)系,同時(shí)研究二者之間的相關(guān)關(guān)系。材料和方法:應(yīng)用免疫組織化學(xué)的方式,將本研究搜集的50例SACC組織標(biāo)本及10例正常唾液腺組織標(biāo)本進(jìn)行染色處理,分別檢測(cè)其中BTBD7及E-cadherin的表達(dá)情況�？笲TBD7抗體結(jié)合位置為細(xì)胞漿,抗E-cadherin抗體結(jié)合位置除細(xì)胞漿外,同時(shí)有細(xì)胞膜;細(xì)胞質(zhì)內(nèi)出現(xiàn)大量的棕黃色顆粒狀物質(zhì)著色判定為BTBD7陽(yáng)性表達(dá)的標(biāo)準(zhǔn),細(xì)胞質(zhì)及細(xì)胞膜表面同時(shí)出現(xiàn)明顯的棕黃色顆粒狀物質(zhì)顯色為E-cadherin陽(yáng)性表達(dá)的標(biāo)準(zhǔn)。用統(tǒng)計(jì)學(xué)軟件SPSS 23.0分析實(shí)驗(yàn)結(jié)果,將50例組織標(biāo)本按照各臨床病理特征(病理類型、神經(jīng)侵犯、血行轉(zhuǎn)移、臨床分期)進(jìn)行分組,采取x2檢驗(yàn)和Fisher確切概率法檢驗(yàn)差異,采取Spearman相關(guān)分析檢驗(yàn)BTBD7及E-cadherin的關(guān)聯(lián)性;結(jié)果以P0.05標(biāo)記為存在統(tǒng)計(jì)學(xué)差異。結(jié)果:(1)與正常唾液腺組織比較,在唾液腺腺樣囊性癌組織中BTBD7表達(dá)顯著增加,在50例腫瘤組織中有21例表達(dá)呈陽(yáng)性,其陽(yáng)性表達(dá)率為420%,兩者之間具有統(tǒng)計(jì)學(xué)差異(P=0.011)。BTBD7在低分化SACC中表達(dá)增加;在Ⅰ期、Ⅱ期與Ⅲ期、Ⅳ期之間具有統(tǒng)計(jì)學(xué)差異(P=0.002)。BTBD7在有神經(jīng)浸潤(rùn)的SACC組織中陽(yáng)性表達(dá)率高達(dá)63.640%,明顯高于無(wú)神經(jīng)浸潤(rùn)的腫瘤組織(21.430%),其差異具有顯著性(P=0.002)。在16例有血行性轉(zhuǎn)移的唾液腺腺樣囊性癌組織中BTBD7的陽(yáng)性表達(dá)率為62.5%,顯著高于無(wú)轉(zhuǎn)移的腫瘤組織,有統(tǒng)計(jì)學(xué)意義(P=0.022)。(2)與正常唾液腺組織比較,在SACC組織中E-cadherin表達(dá)下降(62%),其差異具有統(tǒng)計(jì)學(xué)意義(P=0.018)。E-cadherin在低分化SACC中表達(dá)減少,在Ⅰ期、Ⅱ期與Ⅲ期,Ⅳ期之間的差異具有統(tǒng)計(jì)學(xué)意義(P=0.002)。E-cadherin在有神經(jīng)浸潤(rùn)的SACC組織中陽(yáng)性表達(dá)率為45.45%,明顯低于無(wú)神經(jīng)浸潤(rùn)的癌組織(78.57%),其差異有統(tǒng)計(jì)學(xué)意義(P=0.015)。在有血行性轉(zhuǎn)移的SACC組織中E-cadherin的陽(yáng)性表達(dá)率為37.50%,低于無(wú)轉(zhuǎn)移的癌組織(75.86%),差異具有顯著性(P=0.011)。(3)在50例SACC樣本中,有8例表現(xiàn)為BTBD7與E-cadherin共同陽(yáng)性,6例為共同陰性,二者單獨(dú)陽(yáng)性的例數(shù)分別有23例和6例,經(jīng)過(guò)Spearman相關(guān)分析檢驗(yàn),二者的表達(dá)在SACC中呈現(xiàn)負(fù)相關(guān)(r=-0.419,P=0.002)。結(jié)論:BTBD7在SACC中呈現(xiàn)高表達(dá),E-cadherin呈現(xiàn)低表達(dá),且二者與SACC的病理分期、有無(wú)神經(jīng)浸潤(rùn)及有無(wú)遠(yuǎn)處轉(zhuǎn)移存在相關(guān)性;同時(shí)BTBD7和E-cadherin在SACC組織中的表達(dá)呈現(xiàn)負(fù)相關(guān)。意義:本課題研究了 BTBD7與E-cadherin在唾液腺腺樣囊性癌中的表達(dá)情況,結(jié)果發(fā)現(xiàn)二者與SACC的病理分期、神經(jīng)侵犯、遠(yuǎn)處轉(zhuǎn)移均相關(guān),同時(shí)二者在SACC組織中表達(dá)呈現(xiàn)負(fù)相關(guān),同時(shí)檢測(cè)兩者也許可為研究SACC細(xì)胞的生物學(xué)行為提供參考指標(biāo),為臨床治療SACC提供新思路。
[Abstract]:Objective: to detect the expression of BTBD7 and E-cadherin in salivary adenoid cystic carcinoma (SCC) and to study the relationship between them and the different clinicopathological characteristics of SACC. Materials and methods: 50 SACC tissues and 10 normal salivary gland tissues collected in this study were stained by immunohistochemistry. The expression of BTBD7 and E-cadherin were detected respectively. The binding position of anti-#en1# antibody was cytoplasm, and the binding position of anti-E-cadherin antibody was except cytoplasm. There is cell membrane at the same time; A large number of brown-yellow granules in the cytoplasm were identified as positive expression of BTBD7. The standard of E-cadherin positive expression was observed on the surface of cytoplasm and cell membrane. The results were analyzed by statistical software SPSS 23.0. According to the clinicopathological features (pathological type, nerve invasion, blood metastasis, clinical stage), 50 tissue specimens were divided into two groups. The difference was detected by x2 test and Fisher exact probability method. The correlation between BTBD7 and E-cadherin was examined by Spearman correlation analysis. Results compared with normal salivary gland tissue, the expression of BTBD7 in salivary adenoid cystic carcinoma was significantly increased. Of the 50 cases, 21 were positive, and the positive rate was 420%. There was a significant difference between the two groups in the expression of PTEP 0.011. BTBD7 in poorly differentiated SACC. There was statistical difference between stage 鈪，

本文編號(hào)：1422750