本文關(guān)鍵詞：蜂膠抗腫瘤活性及功效成分研究　出處：《聊城大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

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【摘要】：蜂膠是一味中藥,是由西方蜜蜂采集植物樹脂并混入其上顎腺、蠟腺等分泌物形成的膠黏性物質(zhì)。蜂膠具有廣泛的藥理學(xué)活性,如抗病原微生物、抗氧化、抗炎、抗腫瘤等,目前被普遍應(yīng)用于食品和保健品中以用來預(yù)防疾病和增進(jìn)健康。我們前期實驗表明,EECP具有很好的抗腫瘤活性,但功效成分及作用機(jī)理并不完全清楚,此外,蜂膠不同的提取方法也影響到蜂膠的抗腫瘤活性。基于此,本論文主要研究了(1)EECP和EEBP的抗腫瘤活性比較;(2)在炎癥微環(huán)境下,EECP及其主要組分CAPE的抗腫瘤功效及作用機(jī)理;(3)CPWE的抗腫瘤活性及其功效成分;(4)Ea EP中主要成分的抗腫瘤活性及其功效。研究結(jié)果如下:1.相同劑量的EECP比EEBP抑制腫瘤細(xì)胞增殖作用更明顯。2.在LPS誘導(dǎo)三陰性乳腺癌MDA-MB-231細(xì)胞中,EECP及其主要組分CAPE能夠顯著地抑制乳腺癌細(xì)胞的增殖、遷移。進(jìn)一步研究表明,EECP及其主要組分主要通過下調(diào)TLR4的表達(dá),抑制其下游信號分子My D88、IRAK4、TRIF、NF-κB p65和NO的水平,同時上調(diào)LC3-Ⅱ和p62的蛋白表達(dá),活化細(xì)胞中的凋亡信號分子Caspase 3蛋白,促進(jìn)LPS誘導(dǎo)的MDA-MB-231細(xì)胞的凋亡。3.CPWE共分離得到11種成分(咖啡酸、阿魏酸、異阿魏酸、3,4-二甲氧基肉桂酸、短葉松素、咖啡酸芐酯、CAPE、芹菜素、松屬素、白楊素、高良姜素)。其中四種酚酸類化合物對腫瘤細(xì)胞沒有明顯的抑制作用,兩種酯類和五種黃酮類均具有很好的抑制腫瘤細(xì)胞增殖的作用;進(jìn)一步研究表明,CPWE中主要抗腫瘤組分通過上調(diào)ROS的水平,激活細(xì)胞凋亡信號分子Caspase 3蛋白的表達(dá),促進(jìn)腫瘤細(xì)胞的凋亡。4.通過甲醇-水溶液梯度洗脫Ea EP得到I、II、III、IV、V、VI、VII、VIII八組組分。并研究了抗腫瘤活性發(fā)現(xiàn),蜂膠洗脫部分中III、IV、V具有較強(qiáng)的抗腫瘤功效,通過活化Caspase 3,上調(diào)Annexin7、LC3-Ⅱ等蛋白的表達(dá),下調(diào)p62、4E-BP1、P4E-BP1等蛋白的表達(dá)促進(jìn)腫瘤細(xì)胞的凋亡和自噬。
[Abstract]:Propolis is a traditional Chinese medicine, is the western bee collecting plant resin and mixed with their mandibular gland, adhesive substance wax gland secretions to form. Propolis has wide pharmacological activity, such as anti microbial, anti-inflammatory, antioxidant, anti-tumor, is currently widely used in food and health care products in order to prevent disease and improve health. Our previous experiments showed that, EECP has good antitumor activity, but the effectiveness of the composition and mechanism is not entirely clear, in addition, the different extraction methods of propolis can affect the antitumor activity of propolis. Based on this, this paper mainly studies the comparison of EECP (1) and the antitumor activity of EEBP; (2) in the inflammatory microenvironment, antitumor effect and mechanism of EECP and its main components of CAPE; (3) the anti-tumor activity and the effect of the composition of CPWE; (4) the main component of the Ea EP in the anti-tumor activity and efficacy. The results were as follows: 1. the same dose of EECP than EEBP inhibited the proliferation of tumor cells was more obvious in.2. LPS induced three negative breast cancer MDA-MB-231 cells, EECP and its main components of CAPE can significantly inhibit the proliferation of breast cancer cells, migration. Further study showed that EECP and its main components mainly through down regulating the expression of TLR4 and its downstream signal suppression D88 IRAK4, TRIF molecules My, NF-, p65 kappa B and NO levels, and up regulation of LC3- II and p62 protein expression and activation of apoptotic signal molecules in Caspase cells and 3 protein, promote the apoptosis of.3.CPWE induced by LPS in MDA-MB-231 cells were isolated from 11 ingredients (caffeic acid, ferulic acid and isoferulic acid. 3,4- two methoxy cinnamic acid, Ye Songsu short, caffeic acid benzyl ester, CAPE, apigenin, pinocembrin, chrysin, Takara Jiangso). The four kinds of phenolic compounds have no obvious inhibitory effect on tumor cells, two esters and five yellow Ketones have good inhibitory effects on tumor cells; further study showed that CPWE mainly anticancer components through upregulation of ROS expression level, activation of apoptotic signaling molecules Caspase 3 protein, promote the apoptosis of.4. tumor cells by gradient elution of methanol water solution Ea EP I, II, III, IV V, VI, VII, VIII, eight groups of components. And to study the antitumor activity of propolis, was part of III, IV, V has strong anti-tumor effect, through the activation of Caspase 3, up regulation of Annexin7 expression, LC3- II protein down regulated p62,4E-BP1 expression of P4E-BP1 protein in promoting apoptosis and autophagy the tumor cells.

【學(xué)位授予單位】：聊城大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R285

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