本文關(guān)鍵詞：穩(wěn)定型心絞痛患者中循環(huán)長鏈非編碼RNA（ENST00000589524.1）作為生物標(biāo)記物可能性的研究　出處：《新疆醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

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【摘要】：背景:目前,心血管疾病嚴(yán)重的威脅著人類健康,已經(jīng)成為人類健康的頭號殺手;穩(wěn)定型心絞痛是一種常見的心血管疾病,它主要由心肌灌注與心肌需求平衡失調(diào)引起,其病理基礎(chǔ)幾乎都是冠狀動脈粥樣硬化性狹窄;在過去十年中,發(fā)明并使用了各種靶向治療藥物用來治療心血管疾病,但是在患者生存率方面并未得到相應(yīng)的提高。近幾年來,為了進(jìn)一步降低心血管相關(guān)疾病的死亡率,科學(xué)家們對非編碼RNA(non-coding RNA,nc RNA)與心血管相關(guān)疾病之間的聯(lián)系進(jìn)行了艱苦卓絕的探索與研究,比如長鏈非編碼RNA(long noncoding RNA,lnc RNA)和短鏈非編碼微RNA(micro RNA,mi RNA)。Lnc RNA是含有超過200個核苷酸,能夠調(diào)節(jié)基因表達(dá)的內(nèi)源性RNA。最近的研究提示lnc RNAs在心血管疾病的發(fā)生和發(fā)展過程中起著關(guān)鍵作用。例如,Bvrt,Fendrr,ANRIL,MIAT,LIPCAR和My Heart(Mhrt)在心血管發(fā)育、心臟疾病、心肌梗死、心肌病、心力衰竭和動脈粥樣硬化中起著重要作用。此外,最新的研究表明在許多病理狀態(tài)的發(fā)生和發(fā)展中,lnc RNAs的表達(dá)失調(diào);這提示lnc RNA可能可以成為理想的治療靶點和生物標(biāo)志物。然而,lnc RNA作為穩(wěn)定型心絞痛的生物標(biāo)志物目前沒有任何相關(guān)的研究報道。在前期的lnc RNA微陣列研究中,我們發(fā)現(xiàn)穩(wěn)定型組中l(wèi)nc RNA ENST00000589524.1的表達(dá)明顯增高。目的:本研究通過測定ENST00000589524.1在穩(wěn)定型心絞痛患者及健康人群中的表達(dá)水平,評估ENST00000589524.1是否可作為穩(wěn)定型心絞痛的生物標(biāo)志物,并分析ENST00000589524.1表達(dá)水平與穩(wěn)定型心絞痛患者的相關(guān)危險因素。材料和方法:選取38例穩(wěn)定型心絞痛(stable angina pectoris)患者及38例健康對照組,均來自2014年11月至2015年6月期間相繼在新疆醫(yī)科大學(xué)第一附屬醫(yī)院心血管內(nèi)科住院的患者。采用q RT-PCR方法去測定穩(wěn)定型心絞痛患者和健康對照組患者中ENST00000589524.1的表達(dá)水平。結(jié)果:q RT-PCR分析結(jié)果顯示ENST00000589524.1的表達(dá)水平在穩(wěn)定型心絞痛患者中呈上調(diào)趨勢。在本研究中,我們沒有發(fā)現(xiàn)lnc RNA表達(dá)水平與穩(wěn)定型心絞痛相關(guān)風(fēng)險因素。結(jié)論:ENST00000589524.1是穩(wěn)定型心絞痛的潛在生物標(biāo)志物。
[Abstract]:Background: at present, cardiovascular disease is a serious threat to human health, has become the number one killer of human health; stable angina is a common cardiovascular disease, which is mainly composed of myocardial perfusion and myocardial demand imbalance caused by the pathological basis of almost all coronary atherosclerotic stenosis; in the past ten years, and invention the use of a variety of targeted drugs used in the treatment of cardiovascular disease, but did not get the corresponding increase in the rate of survival. In recent years, in order to further reduce cardiovascular disease mortality, scientists of non encoding RNA (non-coding RNA, NC RNA) to explore and research with extremely hard and bitter cardiovascular diseases for example, a long chain of non encoding RNA (long noncoding RNA, LNC RNA) and short chain non micro RNA (micro encoding RNA, MI RNA).Lnc RNA contains more than 200 nucleotides, A recent study of endogenous RNA. can regulate gene expression suggesting that LNC RNAs plays a key role in the occurrence and development of cardiovascular diseases. For example, Bvrt, Fendrr, ANRIL, MIAT, LIPCAR and My Heart (Mhrt) in the development of cardiovascular, heart disease, myocardial infarction, heart disease, heart failure and plays an important role in atherosclerosis in addition, the latest research shows that the occurrence and development in many pathological conditions, disorder of expression of LNC RNAs; it is suggested that LNC RNA may be an ideal therapeutic targets and biomarkers. However, LNC RNA as a biomarker for stable angina. There are no related research reports. LNC RNA microarray study in the early stage, we found that the expression of LNC RNA ENST00000589524.1 in the stable group was significantly increased. Objective: This study through the determination of ENST00000589524.1 in patients with stable angina pectoris and The expression level of healthy people, to assess whether ENST00000589524.1 can be used as stable angina biomarkers, and analyze the risk in patients with ENST00000589524.1 expression and stable angina. Methods: 38 patients with stable angina pectoris (stable angina pectoris) patients and 38 healthy controls were from November 2014. To June 2015 have in patients with cardiovascular diseases hospitalized in the First Affiliated Hospital of Xinjiang Medical University. To determine stable angina patients and healthy control group the expression level of ENST00000589524.1 in patients with Q RT-PCR method. Results: Q RT-PCR analysis showed that the expression levels of ENST00000589524.1 were up-regulated in patients with angina pectoris in stable type. In this study, we found no LNC RNA expression level in patients with stable angina related risk factors. Conclusion: ENST00000589524. 1 is a potential biomarker of stable angina pectoris.

【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R541.4

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本文編號：1372550