發(fā)布時間：2017-12-27 22:22

  本文關(guān)鍵詞：冠心寧片對大鼠心肌缺血再灌注損傷的心肌保護(hù)作用及機(jī)制研究　出處：《浙江中醫(yī)藥大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 冠心寧片 心肌缺血再灌注損傷 抗氧化 心臟自主神經(jīng)功能 心肌梗死 大鼠

【摘要】：目的觀察冠心寧片(GXNT)對心肌缺血再灌注損傷(MI/RI)大鼠的心肌保護(hù)作用,并探討其作用機(jī)制。方法SD大鼠70只,隨機(jī)分為7組,即偽手術(shù)組、MI/RI組、GXNT 75mg/kg,150mg/kg,300mg/kg,600mg/kg 劑量組和 300mg/kg 復(fù)方丹參片(DST)組,每組10只。所有大鼠均預(yù)防性經(jīng)口灌胃給藥7天后,采用結(jié)扎大鼠左冠狀動脈前降支法,建立MI/RI大鼠模型。期間記錄大鼠心電圖,分析心電圖J點(diǎn)和心率變異性(HRV)指標(biāo);用伊文思藍(lán)(EB)和2,3,5-氯化三苯基四氮唑藍(lán)(TTC)雙重染色心肌并分析心肌梗死面積,測定血漿天門冬氨酸氨基轉(zhuǎn)氨酶(AST)活性、乳酸脫氫酶(LDH)活性、肌酸激酶(CK)及其同工酶(CK-MB)活性、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量和一氧化氮(NO)水平,同時取部分心肌進(jìn)行組織病理HE染色,觀察心肌病理改變情況;另取部分心肌組織進(jìn)行組織勻漿,測定心肌組織中SOD活性、MDA含量、NO水平、髓過氧化物酶(MPO)活性和總抗氧化能力(T-AOC)水平的變化。結(jié)果與偽手術(shù)組比,MI/RI模型大鼠冠脈結(jié)扎后缺血期心電圖J點(diǎn)明顯抬高(P0.01),再灌注后J點(diǎn)雖均有所降低,但仍顯著高于偽手術(shù)組(P0.01),RR間期、SDNN、RMSSD、TP和HFnu均出現(xiàn)明顯降低(P0.05,P0.01),LFnu和LF/HF比值顯著升高(P0.01),同時心肌缺血范圍(ARR/WH和ARR/LV)和梗死范圍(INF/WH、INF/LV和INF/ARR)均顯著增加(P0.01),血漿AST、LDH、CK和CK-MB活性均明顯升高(P0.01),血漿和心肌組織中SOD活性、T-AOC活性和NO活性均明顯降低(P0.05,P0.01),MDA含量和MPO活性均明顯升高(P0.05,P0.01),心肌纖維腫脹、紊亂、呈波浪樣改變、部分心肌纖維斷裂、細(xì)胞核有破裂、溶解消失現(xiàn)象,心肌間質(zhì)有炎性細(xì)胞浸潤,并可見局灶性壞死和出血,提示MI/RI模型大鼠出現(xiàn)明顯的心肌損傷,心肌自由基生成增多,存在明顯的氧化應(yīng)激和炎癥反應(yīng),導(dǎo)致心臟自主神經(jīng)功能紊亂的狀態(tài)。給予冠心寧片和復(fù)方丹參片后能明顯降低再灌注期J點(diǎn)、∑-J值和J點(diǎn)平均值(P0.05,P0.01),顯著降低心肌缺血范圍和梗死面積(P0.05,P0.01),能明顯升高SDNN、RMSSD、TP和HFnu以及降低LFnu和LF/HF比值(P0.05,P0.01),同時能抑制血漿AST、LDH、CK和CK-MB活性的釋放(P0.05,P0.01),提高血漿SOD活性和NO水平(P0.05,P0.01),并能降低MDA含量(P0.05,P0.01),同時能顯著提高心肌組織SOD活性、T-AOC活性、NO水平和降低心肌組織MDA含量和MPO活性(P0.05,P0.01),并能改善心肌病理組織結(jié)構(gòu)。結(jié)論冠心寧片能通過抗氧化和抑制炎癥反應(yīng),改善心臟自主神經(jīng)調(diào)控功能,從而減輕MI/RI大鼠心肌梗死,具有抗MI/RI的作用。
[Abstract]:Objective To observe the effect of Guanxinning tablet (GXNT) on myocardial ischemic reperfusion injury (MI/RI) myocardial protective effect in rats, and to explore its mechanism of action. Methods 70 SD rats were randomly divided into 7 groups, namely, the pseudo operation group, MI/RI group, GXNT 75mg/kg, 150mg/kg, 300mg/kg, 600mg/kg dose group and 300mg/kg Compound Salvia Tablet (DST) group, 10 rats in each group. The rat model of MI/RI was established by ligation of the left anterior descending branch of the coronary artery in all rats after 7 days of prophylactic administration of the oral administration. During the recording of the electrocardiogram of rats, J analysis of ECG and heart rate variability index (HRV); Evans blue (EB) and 2,3,5- chloride three phenyl tetrazolium (TTC) double staining and analysis of myocardial infarct size, determination of plasma aspartate aminotransferase (AST) activity, lactate dehydrogenase (LDH) activity, creatine kinase (CK) and its isoenzyme (CK-MB) activity, superoxide dismutase (SOD) activity, malondialdehyde (MDA) content and nitric oxide (NO) level, at the same time, part of Myocardium Pathological HE staining, observe the changes of cardiomyopathy; another part of the myocardial tissue homogenate in myocardial tissue by SOD the activity, MDA content, NO level, myeloperoxidase (MPO) activity and total antioxidant capacity (T-AOC) level change. The pseudo operation group, MI/RI model of coronary artery ligation in rats after ischemia ECG J point elevation (P0.01), J after reperfusion is decreased, but still higher than the sham operation group (P0.01), SDNN, RMSSD, RR interval, TP and HFnu were significantly reduced (P0.05, P0.01), LFnu and LF/HF were significantly increased (P0.01), and the extent of myocardial ischemia (ARR/WH and ARR/LV) and infarct size (INF/WH, INF/LV and INF/ARR) were significantly increased (P0.01), AST, LDH, CK and plasma CK-MB activity were significantly increased (P0.01), plasma and myocardial tissue SOD activity, T-AOC activity and the activity of NO was significantly decreased (P0.05, P0.01), MDA content and MPO activity were significantly increased (P0.05, P0.01), myocardial fiber swelling and disorder, a wavy, fracture, part of myocardial fiber rupture, nuclear dissolution phenomenon, myocardial interstitial inflammatory cell infiltration, and visible focal Sexual necrosis and hemorrhage showed that MI/RI rats showed obvious myocardial injury, increased free radicals and obvious oxidative stress and inflammatory reaction, leading to cardiac autonomic dysfunction. Given Guanxinning tablet and Compound Salvia Tablet can significantly reduce the reperfusion period of J, -J and J values of sigma point average (P0.05, P0.01), significantly reduced the extent of myocardial ischemia and infarction area (P0.05, P0.01), can significantly increase SDNN, RMSSD, TP and HFnu and the decrease of LFnu and the ratio of LF/HF (P0.05, P0.01) at the same time, can inhibit the plasma AST, LDH, CK and CK-MB activity (P0.05, P0.01) release, improve the activity of plasma SOD and NO levels (P0.05, P0.01), and can reduce the content of MDA (P0.05, P0.01), at the same time can significantly improve the myocardial SOD activity, T-AOC activity, NO level and decrease the content of MDA and the activity of MPO in myocardial tissue (P0.05, P0.01), and can improve the myocardial pathological structure. Conclusion Guanxinning tablet can inhibit inflammatory reaction by anti-oxidation and, improve the regulation of autonomic nerve function, reduce myocardial infarction in MI/RI rats, with anti MI/RI effect.
【學(xué)位授予單位】：浙江中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R285.5

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王木蘭;潘永明;金敏;徐孝平;王德軍;馬全鑫;陳民利;;斑馬魚血栓模型的建立與冠心寧片的干預(yù)作用[J];中國實(shí)驗(yàn)動物學(xué)報;2016年04期

2 黃崇安;何麗娜;孫嘉利;應(yīng)安娜;葉永挺;蔡琪;袁琳波;花春艷;;羥乙基淀粉對大鼠心肌缺血再灌注后無復(fù)流的影響[J];中國病理生理雜志;2016年03期

3 陳輝;鄭敏;葉華山;;心肌缺血再灌注損傷機(jī)制及治療研究進(jìn)展[J];湖北科技學(xué)院學(xué)報(醫(yī)學(xué)版);2015年03期

4 李冀;王秀珍;李在斯;彭宇飛;王艷麗;;中藥治療心肌缺血再灌注損傷的研究進(jìn)展[J];中醫(yī)藥學(xué)報;2015年02期

5 邵瑩;吳啟南;周婧;樂巍;巢建國;;淡竹葉黃酮對大鼠心肌缺血/再灌注損傷的保護(hù)作用[J];中國藥理學(xué)通報;2013年02期

6 王飛;位凱;沈兵;王烈成;孔德虎;胡金蘭;;天麻素預(yù)處理對大鼠心肌缺血再灌注損傷的保護(hù)作用[J];中國藥學(xué)雜志;2012年23期

7 王彩歌;王永霞;;心肌缺血再灌注損傷的研究進(jìn)展[J];世界中西醫(yī)結(jié)合雜志;2012年08期

8 秦曉靜;呂琪;張新寧;陳福泉;李靈芝;張永亮;;蕨麻乙醇提取物抑制大鼠急性心肌缺血再灌注損傷導(dǎo)致的心肌細(xì)胞凋亡[J];中國中藥雜志;2012年09期

9 高楓;孫桂波;任小宇;聶媛媛;孫靜;秦蒙;孫曉波;;丹酚酸B對大鼠離體心臟缺血再灌注損傷的保護(hù)作用[J];中國中藥雜志;2012年03期

10 鞏紅巖;秦元旭;王更富;王慶志;;葛根素對大鼠體外循環(huán)后心肌缺血再灌注損傷的保護(hù)作用及抗氧化應(yīng)激機(jī)制的探討[J];中國實(shí)驗(yàn)方劑學(xué)雜志;2012年01期

，

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