下丘腦神經(jīng)肽（NPY、AgRP、POMC、CART）在大絨鼠體重和能量代謝中調(diào)節(jié)作用的研究
發(fā)布時(shí)間：2019-05-15 18:55
【摘要】：大絨鼠(Eothenomys miletus)為中國特有種,主要分布于橫斷山區(qū)及其附近地區(qū),為橫斷山區(qū)固有種。之前的研究結(jié)果表明瘦素在不同條件下對于大絨鼠的體重和能量代謝調(diào)節(jié)是不一樣的,但是神經(jīng)機(jī)理調(diào)節(jié)還不是很清楚,為了進(jìn)一步了解瘦素在大絨鼠的體重和能量代謝調(diào)節(jié)機(jī)理,本論文從下丘腦神經(jīng)肽基因進(jìn)一步研究瘦素是如何調(diào)節(jié)的,實(shí)驗(yàn)包括季節(jié)性馴化,溫度、光照和食物的單因素實(shí)驗(yàn)以及三個因子的三因素實(shí)驗(yàn),最終來探討下丘腦神經(jīng)肽基因表達(dá)量對大絨鼠能量穩(wěn)態(tài)的調(diào)節(jié)作用,以確定大絨鼠在橫斷山區(qū)的適應(yīng)模式,并為進(jìn)一步研究下丘腦神經(jīng)元在小型哺乳動物中的體重調(diào)節(jié)和能量穩(wěn)態(tài)調(diào)節(jié)奠定了基礎(chǔ)。實(shí)驗(yàn)結(jié)果分為五個部分：1、下丘腦神經(jīng)肽基因表達(dá)量對大絨鼠體重和能量代謝季節(jié)性變化的影響：大絨鼠的體重和體脂均出現(xiàn)了季節(jié)性變化,冬季較高,夏季較低,食物攝入量季節(jié)性差異顯著,在冬季較高,夏季最低。血清瘦素含量也出現(xiàn)了季節(jié)性變化,和體脂變化趨勢類似,瘦素含量與脂肪含量呈正相關(guān)關(guān)系。下丘腦神經(jīng)肽基因NPY和AgRP表達(dá)量季節(jié)性差異顯著,而POMC和CART表達(dá)量差異不顯著。瘦素與NPY和AgRP負(fù)相關(guān),與POMC和CART正相關(guān)。以上結(jié)果表明大絨鼠在季節(jié)性變化過程中冬季降低體重、體脂,增加攝入量來維持生存。瘦素通過作用于下丘腦神經(jīng)肽基因調(diào)節(jié)大絨鼠的體重和能量代謝。2、下丘腦神經(jīng)肽基因表達(dá)量在冷馴化條件下對大絨鼠體重和能量代謝的影響：低溫降低大絨鼠的體重和體脂,增加攝入量。對照組和低溫組的瘦素含量差異顯著,瘦素含量與脂肪含量呈正相關(guān)關(guān)系。下丘腦神經(jīng)肽基因NPY表達(dá)量差異顯著,而AgRP、POMC和CART表達(dá)量差異不顯著。瘦素與NPY負(fù)相關(guān),與CART正相關(guān),與POMC和AgRP不相關(guān)。以上結(jié)果表明低溫可以降低大絨鼠的體重、體脂和瘦素含量,增加攝入量和NPY表達(dá)量。瘦素通過調(diào)節(jié)NPY和CART表達(dá)量來調(diào)節(jié)大絨鼠的體重和能量代謝。3、下丘腦神經(jīng)肽基因表達(dá)量在不同光照條件下對大絨鼠體重和能量代謝的影響：短光降低大絨鼠的體重和體脂,增加攝入量。光照不影響瘦素含量,瘦素含量與脂肪含量呈正相關(guān)關(guān)系。下丘腦神經(jīng)肽基因NPY、AgRP、POMC和CART表達(dá)量差異不顯著。瘦素與NPY負(fù)相關(guān),與POMC、AgRP和CART不相關(guān)。以上結(jié)果表明短光可以降低大絨鼠的體重和體脂,增加攝入量。瘦素通過調(diào)節(jié)NPY表達(dá)量來調(diào)節(jié)大絨鼠的體重和能量代謝。4、下丘腦神經(jīng)肽基因表達(dá)量在限食條件下對大絨鼠體重和能量代謝的影響：限食降低大絨鼠的體重和體脂,增加攝入量。對照組和限食組的瘦素含量差異顯著,瘦素含量與脂肪含量呈正相關(guān)關(guān)系。下丘腦神經(jīng)肽基因NPY、AgRP、POMC和CART表達(dá)量差異顯著。瘦素與NPY和AgRP負(fù)相關(guān),與POMC和CART正相關(guān)。以上結(jié)果表明限食可以降低大絨鼠的體重、體脂和瘦素含量,增加攝入量和下丘腦神經(jīng)肽基因表達(dá)量。瘦素通過調(diào)節(jié)NPY/AgRP和POMC/CART表達(dá)量來調(diào)節(jié)大絨鼠的體重和能量代謝。5、溫度、光照和食物對大絨鼠體重和下丘腦神經(jīng)肽基因表達(dá)量的影響：低溫和限食可以降低體重和體脂,瘦素含量也顯著降低,NPY和AgRP表達(dá)量顯著增加,而POMC和CART表達(dá)量顯著降低,而光照對于以上指標(biāo)影響不顯著。瘦素與體脂、POMC和CART表達(dá)量正相關(guān),與NPY和AgRP表達(dá)量負(fù)相關(guān)。以上結(jié)果表明溫度和食物是影響大絨鼠體重和能量代謝的因素,瘦素在該環(huán)境下通過調(diào)節(jié)下丘腦神經(jīng)肽基因的表達(dá)量來調(diào)節(jié)大絨鼠的能量穩(wěn)態(tài)。綜上所述：大絨鼠在季節(jié)性變化過程中通過調(diào)整下丘腦神經(jīng)肽基因表達(dá)量來調(diào)節(jié)體重和能量代謝,具體表現(xiàn)在：低溫條件下主要通過調(diào)節(jié)NPY和CART表達(dá)量、短光可以刺激NPY表達(dá)量增加,限食可以改變NPY/AgRP和POMC/CART表達(dá)量。對于環(huán)境因子影響作用的大小,低溫和限食使NPY和AgRP表達(dá)量顯著增加,而POMC和CART表達(dá)量顯著降低,而光照對于以上指標(biāo)影響不顯著。大絨鼠在季節(jié)變化和不同環(huán)境因子條件下的下丘腦神經(jīng)肽基因表達(dá)量的變化最終來適應(yīng)橫斷山區(qū)年溫差小,日溫差大,食物資源相對豐富的棲息環(huán)境。
[Abstract]:Eothrenomys miletus is a kind of endemic species in China, which is mainly distributed in Hengdushan District and its adjacent area, and is an inherent species of Hengdushan District. Previous studies have shown that leptin is different under different conditions for the body weight and energy metabolism of the large-wool rats, but the regulation of the nerve mechanism is not clear, in order to further understand the mechanism of the adjustment of the weight and energy metabolism of leptin in the rats, This paper further studies how leptin is regulated from the hypothalamic neuropeptide gene, including the single factor experiment of seasonal acclimatization, temperature, light and food, and the three factor experiment of three factors. Finally, the regulation of the expression of neuropeptide gene in the hypothalamus on the steady state of the energy of the large-wool mice was discussed to determine the adaptive pattern of the large-pile mice in the Hengduan area and lay the foundation for further study of the weight regulation and the steady-state regulation of the hypothalamic neurons in the small mammals. The results of the experiment were divided into five parts:1. The effect of the expression of the hypothalamic neuropeptide gene on the body weight and the seasonal change of energy metabolism: the body weight and body fat of the rats were seasonal, the winter was higher, the summer was lower, and the seasonal difference of the food intake was significant. In winter, the lowest in summer. The serum leptin content also has a seasonal change, and the change of the body fat is similar, and the leptin content is positively related to the fat content. There was a significant seasonal difference in the expression of NPY and AgP in the hypothalamic neuropeptide gene, while the difference between the expression of POMC and CART was not significant. Leptin was negatively correlated with NPY and AgRP, and was positively correlated with POMC and CART. The results showed that in the course of seasonal variation, the rats were able to reduce body weight, body fat and increase the intake to maintain the survival. The effect of leptin on the body weight and energy metabolism of the large-wool rats was regulated by the action of the neuropeptide gene in the hypothalamus. The effect of the expression of the neuropeptide gene on the body weight and energy metabolism of the rats under cold acclimatization was studied. The leptin content in the control group and the low-temperature group was significant, and the leptin content was positively related to the fat content. There was a significant difference in the expression of NPY in the hypothalamic neuropeptide gene, and the difference of the expression of agRP, POMC and CART was not significant. Leptin is negatively associated with NPY and is not associated with CART and is not associated with POMC and AgRP. The results showed that low temperature could reduce the body weight, body fat and leptin content, increase the intake and NPY expression. Leptin regulates the weight and energy metabolism of the rats by regulating the expression of NPY and CART.3. The effect of the expression of the neuropeptide gene in the hypothalamus on the body weight and energy metabolism of the rats under different light conditions: the short light reduces the body weight and body fat of the large-wool and increases the intake. The light does not affect the leptin content, and the leptin content is positively related to the fat content. The expression of NPY, AgRP, POMC and CART in the hypothalamic neuropeptide gene was not significant. Leptin is negatively related to NPY and is not related to POMC, AgRP and CART. The above results show that the short light can lower the body weight and body fat and increase the intake. Leptin regulates the weight and energy metabolism of the rats by regulating the expression of NPY. The leptin content in the control group and the limited group was significant, and the leptin content was positively related to the fat content. The expression of NPY, AgRP, POMC and CART in the hypothalamic neuropeptide gene was significant. Leptin was negatively correlated with NPY and AgRP, and was positively correlated with POMC and CART. The above results show that the limited food can reduce the body weight, body fat and leptin content, increase the intake and the expression of the neuropeptide gene in the hypothalamus. Leptin regulates the weight and energy metabolism of the rats by adjusting the expression of NPY/ AgRP and POMC/ CART. The effects of 5, temperature, light and food on the expression of neuropeptide gene in the body weight and the hypothalamus of the female rats: the low temperature and the limited food can reduce the body weight and body fat, and the leptin content is also significantly reduced. The expression of NPY and AgRP was significantly increased, while the expression of POMC and CART was significantly decreased, and the effect of light on the above indexes was not significant. Leptin was positively correlated with the expression of body fat, POMC and CART, and negatively correlated with the expression of NPY and AgRP. The above results show that the temperature and the food are the factors that affect the body weight and energy metabolism of the rats, and the leptin can regulate the energy homeostasis of the rats by adjusting the expression of the hypothalamic neuropeptide gene in the environment. To sum up, in the course of seasonal variation, the large-pile mouse adjusts the weight and energy metabolism by adjusting the expression amount of the neuropeptide gene in the hypothalamus, which is manifested in that the NPY and the CART expression amount are mainly regulated under the low-temperature condition, and the short light can stimulate the increase of the expression amount of the NPY, The restriction can change the expression of NPY/ AgRP and POMC/ CART. For the effects of environmental factors, the expression of NPY and AgRP was increased significantly, while the expression of POMC and CART was significantly decreased, and the effects of light on the above indexes were not significant. The change of the expression of the hypothalamic neuropeptide gene under the conditions of seasonal variation and different environmental factors is the last to adapt to the small temperature difference, the large temperature difference and the relative abundance of food resources in the Hengdushan region.
【學(xué)位授予單位】：南京師范大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2015
【分類號】：Q493
本文編號：2477727
[Abstract]:Eothrenomys miletus is a kind of endemic species in China, which is mainly distributed in Hengdushan District and its adjacent area, and is an inherent species of Hengdushan District. Previous studies have shown that leptin is different under different conditions for the body weight and energy metabolism of the large-wool rats, but the regulation of the nerve mechanism is not clear, in order to further understand the mechanism of the adjustment of the weight and energy metabolism of leptin in the rats, This paper further studies how leptin is regulated from the hypothalamic neuropeptide gene, including the single factor experiment of seasonal acclimatization, temperature, light and food, and the three factor experiment of three factors. Finally, the regulation of the expression of neuropeptide gene in the hypothalamus on the steady state of the energy of the large-wool mice was discussed to determine the adaptive pattern of the large-pile mice in the Hengduan area and lay the foundation for further study of the weight regulation and the steady-state regulation of the hypothalamic neurons in the small mammals. The results of the experiment were divided into five parts:1. The effect of the expression of the hypothalamic neuropeptide gene on the body weight and the seasonal change of energy metabolism: the body weight and body fat of the rats were seasonal, the winter was higher, the summer was lower, and the seasonal difference of the food intake was significant. In winter, the lowest in summer. The serum leptin content also has a seasonal change, and the change of the body fat is similar, and the leptin content is positively related to the fat content. There was a significant seasonal difference in the expression of NPY and AgP in the hypothalamic neuropeptide gene, while the difference between the expression of POMC and CART was not significant. Leptin was negatively correlated with NPY and AgRP, and was positively correlated with POMC and CART. The results showed that in the course of seasonal variation, the rats were able to reduce body weight, body fat and increase the intake to maintain the survival. The effect of leptin on the body weight and energy metabolism of the large-wool rats was regulated by the action of the neuropeptide gene in the hypothalamus. The effect of the expression of the neuropeptide gene on the body weight and energy metabolism of the rats under cold acclimatization was studied. The leptin content in the control group and the low-temperature group was significant, and the leptin content was positively related to the fat content. There was a significant difference in the expression of NPY in the hypothalamic neuropeptide gene, and the difference of the expression of agRP, POMC and CART was not significant. Leptin is negatively associated with NPY and is not associated with CART and is not associated with POMC and AgRP. The results showed that low temperature could reduce the body weight, body fat and leptin content, increase the intake and NPY expression. Leptin regulates the weight and energy metabolism of the rats by regulating the expression of NPY and CART.3. The effect of the expression of the neuropeptide gene in the hypothalamus on the body weight and energy metabolism of the rats under different light conditions: the short light reduces the body weight and body fat of the large-wool and increases the intake. The light does not affect the leptin content, and the leptin content is positively related to the fat content. The expression of NPY, AgRP, POMC and CART in the hypothalamic neuropeptide gene was not significant. Leptin is negatively related to NPY and is not related to POMC, AgRP and CART. The above results show that the short light can lower the body weight and body fat and increase the intake. Leptin regulates the weight and energy metabolism of the rats by regulating the expression of NPY. The leptin content in the control group and the limited group was significant, and the leptin content was positively related to the fat content. The expression of NPY, AgRP, POMC and CART in the hypothalamic neuropeptide gene was significant. Leptin was negatively correlated with NPY and AgRP, and was positively correlated with POMC and CART. The above results show that the limited food can reduce the body weight, body fat and leptin content, increase the intake and the expression of the neuropeptide gene in the hypothalamus. Leptin regulates the weight and energy metabolism of the rats by adjusting the expression of NPY/ AgRP and POMC/ CART. The effects of 5, temperature, light and food on the expression of neuropeptide gene in the body weight and the hypothalamus of the female rats: the low temperature and the limited food can reduce the body weight and body fat, and the leptin content is also significantly reduced. The expression of NPY and AgRP was significantly increased, while the expression of POMC and CART was significantly decreased, and the effect of light on the above indexes was not significant. Leptin was positively correlated with the expression of body fat, POMC and CART, and negatively correlated with the expression of NPY and AgRP. The above results show that the temperature and the food are the factors that affect the body weight and energy metabolism of the rats, and the leptin can regulate the energy homeostasis of the rats by adjusting the expression of the hypothalamic neuropeptide gene in the environment. To sum up, in the course of seasonal variation, the large-pile mouse adjusts the weight and energy metabolism by adjusting the expression amount of the neuropeptide gene in the hypothalamus, which is manifested in that the NPY and the CART expression amount are mainly regulated under the low-temperature condition, and the short light can stimulate the increase of the expression amount of the NPY, The restriction can change the expression of NPY/ AgRP and POMC/ CART. For the effects of environmental factors, the expression of NPY and AgRP was increased significantly, while the expression of POMC and CART was significantly decreased, and the effects of light on the above indexes were not significant. The change of the expression of the hypothalamic neuropeptide gene under the conditions of seasonal variation and different environmental factors is the last to adapt to the small temperature difference, the large temperature difference and the relative abundance of food resources in the Hengdushan region.
【學(xué)位授予單位】：南京師范大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2015
【分類號】：Q493
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