發(fā)布時(shí)間：2018-06-22 00:13

  本文選題：流產(chǎn)布魯菌 + 毒力相關(guān)基因�。� 參考：《中國(guó)農(nóng)業(yè)科學(xué)院》2016年博士后論文

【摘要】：本研究在前期PCR介導(dǎo)的信號(hào)標(biāo)簽隨機(jī)突變庫(kù)基礎(chǔ)上,以感染小鼠為模型,篩選鑒定流產(chǎn)布魯菌毒力相關(guān)基因。為進(jìn)一步研究布魯菌毒力相關(guān)基因的功能,構(gòu)建了一系列用于布魯菌克隆和表達(dá)外源基因的質(zhì)粒。之后,對(duì)布魯菌一個(gè)LysR家族調(diào)控子的功能進(jìn)行詳細(xì)闡明,為布魯菌致病機(jī)制研究奠定基礎(chǔ)。本文的主要結(jié)論是：1.共獲得56株流產(chǎn)布魯菌減毒突變株,鑒定53個(gè)毒力相關(guān)基因,其中,39個(gè)是本研究中新鑒定的基因。2.注釋53個(gè)毒力相關(guān)基因,功能分類探討相關(guān)基因可能與布魯菌毒力之間的關(guān)系。3.構(gòu)建一系列pTM質(zhì)粒用于在布魯菌中克隆外源基因,包括pTM1-Cm、pTM2-Amp和pTM3-Km。4.構(gòu)建pGH-6x His和pZT-6x His質(zhì)粒,為布魯菌表達(dá)和誘導(dǎo)性表達(dá)外源基因提供條件。5.鑒定一個(gè)與布魯菌毒力相關(guān)的LysR家族轉(zhuǎn)錄調(diào)控子LsrB蛋白,參與調(diào)控布魯菌胞內(nèi)存活和建立慢性感染。6.發(fā)現(xiàn)LsrB調(diào)控布魯菌抵抗氧化應(yīng)激、氮化應(yīng)激、自然血清以及調(diào)控布魯菌逃逸宿主免疫監(jiān)控。7.揭示LsrB在根瘤菌目細(xì)菌中具有保守的共同調(diào)控機(jī)制。
[Abstract]:On the basis of the early PCR mediated random mutation library, this study screened the Virulence Related Genes of Brucella abortus using the infected mice as the model. In order to further study the function of Brucella virulence related genes, a series of plasmid for Brucella cloning and expression of foreign genes was constructed. Then, a LysR family of Brucella was used. The function of the regulator was clarified in detail to lay the foundation for the study of the pathogenic mechanism of Brucella. The main conclusions of this paper were: 1. a total of 56 strains of Brucella abortus were obtained and 53 virulence related genes were identified. Among them, 39 were.2. annotated and 53 Virulence Related Genes of the newly identified gene in this study, and the functional classification was used to explore the possible genes. The relationship with Brucella virulence.3. constructs a series of pTM plasmids to clone foreign genes in Brucella, including pTM1-Cm, pTM2-Amp and pTM3-Km.4. to construct pGH-6x His and pZT-6x His plasmids, providing conditions for the expression and inducible expression of foreign genes for Brucella, and.5. to identify a LysR family transcriptional regulator associated with Brucella virulence SrB protein, participating in the regulation of Brucella cell memory and the establishment of chronic infection.6. found that LsrB regulated Brucella to resist oxidative stress, nitridation stress, natural serum and control of the immune monitoring of Brucella escaping host immune monitoring.7. revealed that LsrB has a conservative control mechanism in Rhizobium bacteria.
【學(xué)位授予單位】：中國(guó)農(nóng)業(yè)科學(xué)院
【學(xué)位級(jí)別】：博士后
【學(xué)位授予年份】：2016
【分類號(hào)】：Q93

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