本文選題：嗜神經(jīng)病毒　切入點：情緒和記憶　出處：《中國科學(xué)院大學(xué)(中國科學(xué)院武漢物理與數(shù)學(xué)研究所)》2016年博士論文　論文類型：學(xué)位論文

【摘要】：繪制大腦神經(jīng)網(wǎng)絡(luò)的連接圖譜有助于我們更好地解析大腦的功能,發(fā)展用于繪制大腦神經(jīng)網(wǎng)絡(luò)結(jié)構(gòu)的技術(shù),已成為當(dāng)前神經(jīng)科學(xué)研究的迫切需求。傳統(tǒng)的神經(jīng)環(huán)路研究的方法主要包括高爾基染色以及蛋白質(zhì)或染料標(biāo)記的方法。基于病毒發(fā)展而來的神經(jīng)環(huán)路示蹤技術(shù),是目前繪制大腦連接圖譜的常用手段之一,而且也是近年來神經(jīng)科學(xué)研究中發(fā)展十分迅速的領(lǐng)域。目前常用的用于標(biāo)記神經(jīng)環(huán)路的嗜神經(jīng)病毒主要有偽狂犬病毒(pseudorabies virus,PRV)、重組狂犬病毒(rabiesvirus,RV)、水皰性口炎病毒(vesicularstomatitisvirus,VSV)和單純皰疹病毒(herpes simplex virus,HSV),這些嗜神經(jīng)病毒可以用于解析大腦中樞特定核團或者外周器官的單級或多級的輸入輸出網(wǎng)絡(luò)。另外一些常用的解析神經(jīng)環(huán)路的病毒包括腺相關(guān)病毒(adeno-associatedvirus,AAV)等病毒,可以用于神經(jīng)環(huán)路的操控以及神經(jīng)元投射等研究。利用上述這些病毒可以解析目前神經(jīng)科學(xué)研究中的部分問題,本研究利用上述病毒標(biāo)記了海馬和杏仁核的環(huán)路以及神經(jīng)元的精細形態(tài)的描繪。主要內(nèi)容是:1、協(xié)助實驗室建立并完善了用于解析神經(jīng)環(huán)路的多種病毒系統(tǒng)。2、合作研究完成習(xí)得性絕望和習(xí)得性充滿希望兩種情感對小鼠空間記憶的不同的影響的神經(jīng)環(huán)路機制。通過對比不同情緒狀態(tài)下,小鼠空間學(xué)習(xí)記憶能力的變化,從而鑒定出基底外側(cè)杏仁核靠后部(BLP)和腹側(cè)海馬CA1的單突觸連接調(diào)控了情緒對空間學(xué)習(xí)記憶能力的影響,該部分工作主要與華中科技大學(xué)同濟醫(yī)學(xué)院王建枝教授課題組合作完成。3、合作研究完成了稀疏標(biāo)記小鼠大腦神經(jīng)元并追蹤重構(gòu)單個神經(jīng)元的方法,用AAV病毒稀疏標(biāo)記小鼠大腦多個腦區(qū)的神經(jīng)元的形態(tài)并使用fMOST(華中科技大學(xué)光電國家實驗室技術(shù))對外側(cè)前額葉進行精細結(jié)構(gòu)成像,獲得了小鼠外側(cè)前額葉的稀疏標(biāo)記的神經(jīng)元的全腦投射數(shù)據(jù)。該部分工作主要是和華中科技大學(xué)光電國家實驗室張玉慧教授課題組和曾紹群教授課題組合作完成。4、合作研究了小鼠海馬區(qū)苔蘚細胞(Mossy cell)的精細形態(tài),主要方法是利用上述的可以用于稀疏標(biāo)記的AAV病毒注射海馬DG區(qū),該病毒可以選擇性地稀疏標(biāo)記海馬DG區(qū)的苔蘚細胞,通過該方法可以獲得苔蘚細胞的精細形態(tài)結(jié)構(gòu),該部分工作主要是和華中科技大學(xué)光電國家實驗室張玉慧教授課題組和曾紹群教授課題組合作完成。
[Abstract]:Mapping the connections of neural networks in the brain will help us better analyze the functions of the brain and develop techniques for mapping the network structure of the neural network of the brain. It has become an urgent need to study neuroscience. The traditional methods of neural loop research mainly include Golgi staining and protein or dye labeling. Neural loop tracing technology based on virus development. Is one of the most common methods of mapping brain connections. It is also a rapidly developing field of neuroscience research in recent years. Currently, the commonly used neuroviruses used to label neural loops are pseudorabies virus (PRV), Recombinant rabies virus (RV), vesicularis stomatitis virus (VSVV) and single rabies virus (RV). Herpes simplex virus.These neuroviruses can be used to parse single-stage or multi-stage input-output networks in specific nuclei or peripheral organs of the brain. Other commonly used viruses that parse nerve loops include viruses such as adeno-associated virus, adeno-associated virus, or adeno-associated virus. These viruses can be used to study neural loop manipulation and neuronal projection. These viruses can be used to analyze some of the problems in current neuroscience research. This study used the virus to label the loop of hippocampus and amygdala and the description of fine morphology of neurons. The main content of this study is: 1, to assist the laboratory to establish and improve a variety of virus systems used to analyze the neural loop. To study the neural loop mechanism of the different effects of the two emotions on the spatial memory of mice, namely, learning despair and learning hopefulness. The changes of spatial learning and memory ability in mice showed that the effects of emotion on spatial learning and memory were regulated by the single synaptic connections between the basolateral amygdaloid nucleus and the ventral hippocampal CA1. This part mainly works with Wang Jianchi Group of Tongji Medical College of Huazhong University of Science and Technology to study the method of sparse labeling mouse brain neurons and tracking the reconstruction of single neurons. The morphology of neurons in multiple brain regions of mice was labeled sparsely with AAV virus and fine structure imaging of the lateral prefrontal lobe was performed using fMOST (Optoelectronic National Laboratory of Huazhong University of Science and Technology). The global brain projection data of sparsely labeled neurons in the lateral prefrontal lobe of mice were obtained. This part of the work is mainly done in collaboration with Professor Zhang Yuhui, National Laboratory of Optoelectronics, Huazhong University of Science and Technology, and Professor Zeng Shaoqun. 4. The fine morphology of mossy cells in the hippocampus of mice was studied. The main method is to inject the DG region of hippocampus with the above-mentioned AAV virus which can be used for sparse labeling. The virus can selectively and sparsely label the mossy cells in the DG region of the hippocampus. By this method, the fine morphology of the mossy cells can be obtained. The main work of this part is to cooperate with Professor Zhang Yuhui and Professor Zeng Shaoqun of National Laboratory of Optoelectronics of Huazhong University of Science and Technology (Huazhong University of Science and Technology).
【學(xué)位授予單位】：中國科學(xué)院大學(xué)(中國科學(xué)院武漢物理與數(shù)學(xué)研究所)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2016
【分類號】：R338

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