發(fā)布時(shí)間：2018-10-09 07:38

【摘要】：多巴胺(DA)、抗壞血酸(AA)、尿酸(UA)是人體新陳代謝中三種重要的生物小分子。人體中這些物質(zhì)含量的高低直接影響人的身體健康。如,缺乏多巴胺可導(dǎo)致精神分裂、帕金森病等,缺乏抗壞血酸可導(dǎo)致壞血病,尿酸代謝紊亂則是引起痛風(fēng)的原因。能夠?qū)θN物質(zhì)進(jìn)行精確檢測(cè),對(duì)這些疾病的診斷和預(yù)防具有重要意義。但三者氧化峰電位卻十分接近,使用普通的電極會(huì)互相干擾,因此各種修飾電極應(yīng)運(yùn)而生。本文制備了三種新型修飾電極,利用循環(huán)伏安法(CV)和微分脈沖伏安法(DPV)對(duì)DA、AA和UA在三種修飾電極上的電化學(xué)響應(yīng)進(jìn)行分析:(1)通過非共價(jià)修飾的方法將聚合離子液體修飾于多壁碳納米管上,不僅可以使易發(fā)生纏繞的多壁碳納米管在水溶液中分散,還可以保留碳納米管的優(yōu)良特性,聚合離子液體的引入也增強(qiáng)了電子轉(zhuǎn)移速率�；诖藢⒕酆想x子液體(聚(1-乙烯基-3-乙基咪唑溴代鹽))和多壁碳納米管結(jié)合,利用該復(fù)合物制備出聚合離子液體-多壁碳納米管修飾電極(Poly(ViEt Im~+Br~-)/MWCNTs/GCE),并采用紅外光譜(FT-IR)和透射電鏡(TEM)等手段對(duì)Poly(ViEt Im~+Br~-)/MWCNTs復(fù)合物進(jìn)行了表征。Poly(Vi Et Im~+Br~-)與MWCNTs實(shí)現(xiàn)了復(fù)合,而且多壁碳納米管在形成復(fù)合物后未發(fā)生纏繞,可穩(wěn)定分散于水溶液中。此外,碳納米管的催化作用與復(fù)合物的協(xié)同作用使得Poly(ViEtIm~+Br~-)/MWCNTs/GCE與MWCNTs/GCE相比,對(duì)DA、AA和UA具有更好的電化學(xué)響應(yīng)信號(hào)。在pH=7.4的PBS中,DA,AA和UA的檢測(cè)范圍分別為2-180μM,40-5000μM和4-50μM。同時(shí)可實(shí)現(xiàn)維生素C注射劑和鹽酸多巴胺注射劑中AA和DA的檢測(cè)。(2)介孔硅(MCF)具有大比表面積,孔徑大小可調(diào)等介孔材料特性,此外,其互通的三維孔道結(jié)構(gòu)還可增強(qiáng)物質(zhì)在MCF孔道中的傳輸和擴(kuò)散�；谠搩�(yōu)勢(shì),本文以介孔硅(MCF)為修飾材料,與石墨等材料混合制備出介孔硅-碳糊電極(MCF/CPE)。實(shí)驗(yàn)結(jié)果顯示,MCF/CPE在p H=6的PBS中,可以在AA、DA和UA共存條件下選擇性測(cè)定DA和UA。在三者同時(shí)存在下,DA和UA的檢測(cè)范圍分別為2-220μM和1-120μM。(3)以離子液體修飾介孔硅(MCF-IL)為電極修飾材料,在MCF優(yōu)良特性的基礎(chǔ)上引入離子液體,制備出碳糊電極(MCF-IL/CPE)。相比MCF/CPE對(duì)AA、UA具有更好的響應(yīng)。MCF-IL(17%)/CPE在p H=6的PBS中,可以在三者共存條件下選擇性測(cè)定DA和UA。DA、UA的檢測(cè)范圍為5-160μM和1-160μM。MCF-IL(25%)/CPE在pH=6的PBS中,實(shí)現(xiàn)了AA、DA、UA的同時(shí)測(cè)定。AA的檢測(cè)范圍為50-3000μM,DA的檢測(cè)范圍為2-180μM,UA的檢測(cè)范圍為1-150μM,并應(yīng)用于樣品中AA和DA的檢測(cè)。
[Abstract]:Dopamine (DA), ascorbic acid (AA), uric acid (UA) is one of the three important biomolecules in human metabolism. The content of these substances in the human body directly affects the health of the human body. For example, lack of dopamine can lead to schizophrenia, Parkinson's disease, lack of ascorbic acid can lead to scurvy, uric acid metabolism disorder is the cause of gout. It is important to diagnose and prevent these diseases by accurate detection of three kinds of substances. However, the oxidation peak potentials of the three kinds of electrodes are very close, and the common electrodes interfere with each other, so all kinds of modified electrodes emerge as the times require. In this paper, three novel modified electrodes were prepared. The electrochemical responses of DA,AA and UA on three modified electrodes were analyzed by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). (1) Polymeric ionic liquids were modified onto multi-walled carbon nanotubes by non-covalent modification. It can not only disperse the multi-walled carbon nanotubes in aqueous solution, but also retain the excellent properties of carbon nanotubes. The introduction of polymeric ionic liquids also increases the electron transfer rate. Based on this, polymeric ionic liquids (poly (1-vinyl-3-ethylimidazolium bromide) and multi-walled carbon nanotubes were combined, The polymeric ionic liquid-multi-wall carbon nanotube modified electrode (Poly (ViEt Im~ Br~-) / MWCNTs / GCE) was prepared. The composite of Poly (ViEt Im~ Br~-) / MWCNTs was characterized by IR (FT-IR) and transmission electron microscopy (TEM). Moreover, the multiwalled carbon nanotubes were not wound after the formation of the complex, and could be dispersed stably in aqueous solution. In addition, Poly (ViEtIm~ Br~-) / MWCNTs / GCE has better electrochemical response to DA,AA and UA than MWCNTs/GCE. In the PBS of pH=7.4, the detection ranges of DAA AA and UA were 2-180 渭 M 40-5000 渭 M and 4-50 渭 M, respectively. At the same time, the detection of AA and DA in vitamin C injection and dopamine hydrochloride injection can be realized. (2) Mesoporous silicon (MCF) has large specific surface area, adjustable pore size and other mesoporous materials. The interworking 3D pore structure can also enhance the transport and diffusion of matter in MCF channels. Based on this advantage, mesoporous silicon-carbon paste electrode (MCF/CPE) was prepared by mixing mesoporous silicon (MCF) with graphite and other materials. The experimental results show that MCFR / CPE can selectively determine DA and UA. under the condition of coexistence of AA,DA and UA in the PBS of pHf6. The detection ranges of DA and UA were 2-220 渭 M and 1-120 渭 M respectively. (3) carbon paste electrode (MCF-IL/CPE) was prepared by using ionic liquid modified mesoporous silicon (MCF-IL) as electrode modification material and introducing ionic liquid on the basis of the excellent properties of MCF. MCF-IL (17%) / CPE can selectively determine the detection range of DA and UA.DA,UA between 5-160 渭 M and 1-160 渭 M.MCF-IL (25%) / CPE in pH=6 PBS. The detection range of AA,DA,UA for simultaneous determination of .AA is 50-3000 渭 m ~ (-1) DA. The detection range is 2-180 渭 M ~ (-1) 渭 m ~ (-1), and it is applied to the detection of AA and DA in the sample.
【學(xué)位授予單位】：沈陽(yáng)師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：O657.1

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