發(fā)布時間：2018-09-09 13:10

【摘要】：紫蘇是我國傳統(tǒng)的中藥材和油料作物,紫蘇籽含油量高,皮中含有原花青素,對紫蘇籽進行綜合利用市場前景廣闊。本文以紫蘇籽為原料,采用超聲波輔助水酶法提取紫蘇籽油,優(yōu)化工藝,并測定紫蘇籽油的性質(zhì);同時研究了紫蘇籽皮原花青素的提取及純化工藝,并對其抗氧化活性進行了測定。主要研究結(jié)果如下:(1)在單因素試驗基礎(chǔ)上,通過響應(yīng)面法得到超聲波輔助水酶法提取紫蘇籽油的最優(yōu)條件:復合酶配比(纖維素酶用量5.5%、中性蛋白酶用量4.5%、果膠酶用量7.5%),液料比6:1(m L/g),超聲時間23min,酶解溫度50℃,酶解時間2.5h,出油率為30.45%。(2)對水酶法、熱榨法、冷榨法及有機溶劑浸提法所提取的紫蘇籽油的主要理化性質(zhì)、脂肪酸組成、生物活性成分含量以及抗氧化能力進行了測定與比較,結(jié)果表明:水酶法油的過氧化值最低,碘值最高;水酶法油的棕櫚酸含量低,油酸含量顯著高于熱榨油與有機溶劑浸提油,α-亞麻酸與不飽和脂肪酸含量最高;水酶法油的VE含量與有機溶劑浸提油相差不大,多酚、總黃酮及甾醇含量最高;通過DPPH法、ABTS法和FRAP法得出水酶法油的抗氧化能力最強。綜合來看,水酶法油的品質(zhì)較好。(3)通過單因素和響應(yīng)面法試驗,得到超聲波輔助法提取紫蘇籽皮原花青素的最佳條件為:液料比15:1(m L/g),乙醇體積分數(shù)62%,超聲時間30min,超聲溫度53℃,提取量為2.170mg/g。(4)通過靜態(tài)吸附與解吸附試驗,選擇XDA-8樹脂來純化紫蘇籽皮原花青素,經(jīng)動態(tài)吸附與解吸附試驗得出純化的最優(yōu)條件為:上樣濃度4mg/mL,上樣流速4BV/h,乙醇體積分數(shù)70%,洗脫流速2BV/h,洗脫劑的用量為13BV。純化前的紫蘇籽皮原花青素含量為5.25%,純化后為12.10%,提高了130.48%。對純化前后的紫蘇籽皮原花青素、VC以及BHT四種樣品的抗氧化能力進行測定與比較,發(fā)現(xiàn)純化后的紫蘇籽皮原花青素的抗氧化能力最強。
[Abstract]:Perilla is a traditional Chinese medicinal material and oil crop in China. Perilla seed has a high oil content and contains procyanidins in the skin, so the comprehensive utilization of perilla seed has a broad market prospect. In this paper, perilla seed oil was extracted from perilla seed by ultrasonic assisted water enzymatic method, and the properties of perilla seed oil were determined, and the extraction and purification of proanthocyanidins from perilla seed skin were studied. The antioxidant activity was determined. The main results are as follows: (1) on the basis of single-factor test, The optimum conditions for extracting perilla seed oil by ultrasonic assisted water enzymatic extraction were obtained by response surface method: ratio of complex enzymes (5. 5% of cellulase, 4.5% of neutral protease, 7.5% of Pectinase), liquid to material ratio of 6:1 (mL / g), ultrasonic time of 23 min, enzymatic hydrolysis temperature of 50 鈩，

本文編號：2232475