發(fā)布時(shí)間：2018-04-29 16:56

  本文選題：枯草芽孢桿菌B67 + 木霉菌Tr8�。� 參考：《沈陽(yáng)農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：為明確枯草芽孢桿菌B 67對(duì)黃瓜幼苗生長(zhǎng)發(fā)育影響。我們采用水培法和Spot比色法,分別探究不同濃度枯草芽孢桿菌對(duì)黃瓜苗期地上部(平均直徑、莖長(zhǎng)、干重、鮮重)、地下部(根長(zhǎng)、根表面積、根體積、根平均直徑、須根數(shù)、根鮮重和干重)、根冠比的影響以及B 67菌液中生長(zhǎng)素濃度的含量。濃度為20%枯草芽孢菌液2處理次的黃瓜幼苗長(zhǎng)勢(shì)良好,處理后21天平均直徑4.88 mm,株高11.15 cm,根長(zhǎng)21.65 cm,根冠比0.94,與各處理結(jié)果均差異顯著,B67菌液中生長(zhǎng)素含量約為8.42ppm。不同濃度B67菌液對(duì)黃瓜苗期促生作用呈正態(tài)分布,濃度為20%枯草芽孢桿菌B 67菌液,施藥2次(間隔期7 d)促生效果最佳,為指導(dǎo)B 67菌液在肥料中添加及創(chuàng)制新型農(nóng)藥提供理論依據(jù)。健根寶粉劑是沈陽(yáng)農(nóng)業(yè)大學(xué)生物農(nóng)藥工程中心自主研發(fā),由綠色木霉菌Tr8和枯草芽孢桿菌B67復(fù)配而成新型生物制劑。為將其制成對(duì)環(huán)境友好的水分散粒劑,對(duì)不同的載體、分散劑、潤(rùn)濕劑、崩解劑、粘結(jié)劑進(jìn)行篩選根據(jù)B67和Tr8對(duì)不同載體、助劑生物相相容性以及其質(zhì)量參數(shù)的影響,篩選出最佳配方為潤(rùn)濕劑:皂角粉;分散劑:羧甲基纖維素鈉;崩解劑:羧甲基淀粉鈉;粘結(jié)劑:聚乙二醇4000,含量分別為4%、5%、3%、4%,載體為硅藻土補(bǔ)足100%。按照國(guó)家對(duì)農(nóng)藥劑型的檢測(cè)標(biāo)準(zhǔn),經(jīng)檢測(cè),健根寶水分散粒劑產(chǎn)品的含孢量約5.31×108/g,含菌量約1.60×1010cfu,潤(rùn)濕時(shí)間約為24s,懸浮率約為83.25%,崩解時(shí)間約為81.91s,含水量約為1.5%,pH約為6.50,各項(xiàng)指標(biāo)均符合國(guó)家標(biāo)準(zhǔn)。初步明確健根寶水分散粒劑的防治效果,按照藥土比1:50進(jìn)行室內(nèi)試驗(yàn),由試驗(yàn)結(jié)果可知,第一次施藥后14天的防治效果為75.02%,第二次施藥后14d的防治效果為65.95%,,健根寶水分散粒劑對(duì)黃瓜枯萎病防治效果較好且防效要顯著高于其試驗(yàn)組,可以用預(yù)防控制黃瓜枯萎病的發(fā)生。
[Abstract]:To determine the effect of Bacillus subtilis B 67 on the growth and development of cucumber seedlings. We studied the effects of Bacillus subtilis (Bacillus subtilis) at different concentrations on shoot (mean diameter, stem length, dry weight, fresh weight, underground part (root length, root surface area, root volume, root average diameter, fibrous root number) of cucumber seedlings by water culture and Spot colorimetry, respectively. The effects of fresh and dry weight of root, the ratio of root to shoot and the content of auxin in B67 liquid. The cucumber seedlings treated with 20% Bacillus subtilis solution for 2 times had a good growth. The average diameter, plant height, root length and shoot ratio were 4.88 mm, 11.15 cm, 21.65 cm and 0.94, respectively. The content of auxin in the solution was about 8.42 ppm. The effects of different concentrations of B67 bacteria on the growth of cucumber seedlings were normal distribution, the concentration of 20% Bacillus subtilis B67 was 20%, and the best effect was 2 times (interval 7 days). It provides theoretical basis for adding and creating new pesticide in fertilizer. Jiangenbao powder was developed by Shenyang Agricultural University Biopesticide Engineering Center. It was prepared from Trichoderma viride (Tr8) and Bacillus subtilis B67 (Bacillus subtilis). In order to prepare water dispersible particles that are friendly to the environment, different carriers, dispersants, wetting agents, disintegrants and binders were selected according to the effects of B67 and Tr8 on the biocompatibility and quality parameters of different carriers and auxiliaries. The best formula was selected as wetting agent: soap powder; dispersant: sodium carboxymethyl cellulose; disintegrating agent: sodium carboxymethyl starch; binder: polyethylene glycol 4000. In accordance with the national standards for the detection of pesticide formulations, The spore content, bacteria content, wetting time, suspension rate, disintegration time and pH value of Jiangenbao water dispersible granules were about 5.31 脳 108 / g, 1.60 脳 10 ~ (10) c / g, 24 s, 83.25 s, 81.91 s and 6.50, respectively. The control effect of Jiangenbao water dispersible granule was preliminarily determined, and the laboratory test was carried out according to the ratio of medicine to soil at 1:50. The control effect was 75.02 on the 14th day after the first application and 65.95on the 14th day after the second application. The effect of Jiangenbao water dispersible granule on the control of cucumber wilt was better and the control effect was significantly higher than that of the experimental group. It can be used to prevent and control cucumber wilt.
【學(xué)位授予單位】：沈陽(yáng)農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TQ450.6
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本文編號(hào)：1820786