本文選題：乳酸菌 + 復(fù)合凝聚��； 參考：《湖北工業(yè)大學(xué)》2017年碩士論文

【摘要】：乳酸菌產(chǎn)品的常溫儲(chǔ)藏是食品工業(yè)亟待解決的熱點(diǎn)問(wèn)題。乳酸菌常溫儲(chǔ)藏降低了產(chǎn)品運(yùn)輸和儲(chǔ)藏成本,且配送、分銷、消費(fèi)者食用等環(huán)節(jié)更方便易行,因而更符合實(shí)際需求。微膠囊技術(shù)可提高乳酸菌對(duì)溫度、濕度、氧氣等環(huán)境因素的脅迫抗性,一直是食品科學(xué)的研究熱點(diǎn)。復(fù)合凝聚法和反溶劑法是近年發(fā)展起來(lái)的新型微囊化技術(shù),本論文擬研究這兩種技術(shù)對(duì)干態(tài)乳酸菌常溫定濕儲(chǔ)藏的適用性,考察制備因素和儲(chǔ)藏因素對(duì)乳酸菌儲(chǔ)藏效果的影響規(guī)律。本研究采用WPI/GA復(fù)合凝聚法包埋乳酸菌,研究了WPI/GA復(fù)合凝聚法包埋對(duì)乳酸菌的常溫定濕儲(chǔ)藏保護(hù)效果;通過(guò)GE/GA復(fù)合凝聚提高復(fù)合凝聚pH,研究了GE/GA復(fù)合凝聚法包埋對(duì)乳酸菌的常溫定濕儲(chǔ)藏保護(hù)效果;反溶劑法構(gòu)建了殼核結(jié)構(gòu)的油/Zein微米顆粒,并研究了其對(duì)乳酸菌的保護(hù)效果。主要結(jié)論如下:(1)優(yōu)化了WPI/GA復(fù)合凝聚工藝,探究了WPI/GA復(fù)合凝聚法包埋對(duì)乳酸菌噴霧干燥后存活率的影響,及不同溫度、濕度條件下乳酸菌的儲(chǔ)藏穩(wěn)定性影響。WPI/GA最佳復(fù)合凝聚條件為pH=4.5、WPI/GA配比為4、總濃度為3%(w/w)。與WPI組相比,WPI/GA組對(duì)乳酸菌噴霧干燥和常溫定濕儲(chǔ)藏中的保護(hù)效果并不顯著,WPI/GA/蔗糖組顯著提高了菌體在噴霧干燥過(guò)程中的存活率,從56.3±8.0%提高至78.7±5.7%,但沒(méi)有明顯改善菌體儲(chǔ)藏穩(wěn)定性。這可能是由于WPI/GA體系復(fù)合凝聚pH較低,在噴霧干燥過(guò)程中加劇了對(duì)菌體的酸傷害和熱傷害,蔗糖在噴霧干燥過(guò)程中發(fā)揮了一定的乳酸菌保護(hù)效果,但未能在常溫定濕儲(chǔ)藏中有效阻止菌體存活惡化。(2)優(yōu)化了GE/GA復(fù)合凝聚工藝,探究了GE/GA復(fù)合凝聚法包埋對(duì)乳酸菌噴霧干燥后存活率的影響,及不同溫度、濕度條件下乳酸菌的儲(chǔ)藏穩(wěn)定性影響。GE/GA最佳復(fù)合凝聚條件為pH=5.0或5.5、GE/GA配比為4、總濃度為2%(w/w)。相比WPI/GA體系,GE/GA復(fù)凝聚明顯提高了復(fù)合凝聚pH。與GA_(5.5)組相比,GE/GA_(5.5)組對(duì)乳酸菌噴霧干燥和常溫定濕儲(chǔ)藏中具有明顯保護(hù)效果;與GE5.5組相比,GE/GA_(5.5)組對(duì)乳酸菌噴霧干燥和常溫定濕儲(chǔ)藏中的保護(hù)效果并不顯著;相比GE/GA_(5.0)組,復(fù)合凝聚pH的提高可以改善GE/GA_(5.5)組菌體儲(chǔ)藏穩(wěn)定性。GE/GA/S5.5組顯著提高了菌體在噴霧干燥過(guò)程中的存活率,從102.6±5.9%提高至131.3±8.0%,且明顯改善菌體儲(chǔ)藏穩(wěn)定性,微膠囊在30oC、11%RH下儲(chǔ)藏56 d后乳酸菌數(shù)量?jī)H下降1.63個(gè)對(duì)數(shù)。(3)優(yōu)化了Zein反溶劑法制備油/Zein微米顆粒的最佳工藝條件,以中鏈甘油三酯(MCT)和棕櫚油(PO)為代表性油脂,研究了油/Zein微米顆粒對(duì)乳酸菌的包埋效果,及微膠囊的結(jié)構(gòu)和理化性質(zhì)。基于Zein反溶劑法,以GA為O/W乳化劑和Zein微米顆粒水相分散劑,制備了具有良好穩(wěn)定性及明顯殼核結(jié)構(gòu)的MCT/Zein微米顆粒。通過(guò)反溶劑法制備的LAB/MCT/Zein微米顆粒和LAB/PO/Zein微米顆粒形態(tài)良好、分散性好,對(duì)乳酸菌實(shí)現(xiàn)了包埋,但由于乙醇的存在造成乳酸菌的存活較低。
[Abstract]:The storage of lactic acid bacteria products at room temperature is a hot issue to be solved in food industry. The storage of lactic acid bacteria at room temperature reduces the cost of product transportation and storage, and distribution, distribution, consumer consumption and other links are more convenient and easy to carry out, thus more in line with the actual needs. Microencapsulation can improve the stress resistance of lactic acid bacteria to environmental factors such as temperature, humidity and oxygen. Compound coagulation and antisolvent are new microencapsulation techniques developed in recent years. In this paper, the applicability of these two techniques to dry lactic acid bacteria storage at room temperature and humidity was studied. The effects of preparation factors and storage factors on the storage effect of lactic acid bacteria were investigated. In this study, lactic acid bacteria were encapsulated by WPI/GA complex coagulation method, and the protective effect of WPI/GA composite coagulation method on lactic acid bacteria stored at room temperature and humidity was studied. The protective effect of GE/GA composite coagulation method on the storage of lactic acid bacteria at room temperature and humidity was studied by raising the pH of complex coagulation by GE/GA complex coagulation, and the oil / Zein micron particles with shell core structure were constructed by anti-solvent method, and the protective effect of GE/GA on lactic acid bacteria was studied. The main conclusions are as follows: (1) the process of WPI/GA compound coagulation was optimized, and the effect of WPI/GA composite coagulation method on survival rate of lactic acid bacteria after spray drying and different temperatures were investigated. The effect of storage stability of lactic acid bacteria on the storage stability of lactic acid bacteria. The optimum conditions of WPI / GA were as follows: pH = 4.5% WPI / GA = 4, and the total concentration of WPI / GA = 3 / W ~ (-1). Compared with WPI group, the protective effect of WPI / GA group on lactic acid bacteria spray drying and storage at room temperature and humidity was not significant. WPI / GA / sucrose group significantly increased the survival rate of the bacteria during spray drying from 56.3 鹵8.0% to 78.7 鹵5.7%, but did not significantly improve the storage stability. This may be due to the low pH value of compound condensation in WPI/GA system, which increases the acid and thermal injury to the bacteria during spray drying, and sucrose plays a protective role in the spray drying process by lactic acid bacteria, while in the process of spray drying, sucrose plays an important role in the protection of lactic acid bacteria. But it failed to effectively prevent the deterioration of cell survival in normal temperature and humidity storage.) the process of GE/GA compound coagulation was optimized, and the effect of GE/GA compound coagulation method on survival rate of lactic acid bacteria after spray drying and different temperatures were investigated. Under the condition of humidity, the effect of storage stability of lactic acid bacteria. GE / GA is as follows: pH=5.0 or 5.5% GE / GA ratio is 4, total concentration is 2 w/ w / w ~ (-1). Compared with WPI/GA system, GE / GA complex coacervation improved the complex coagulation pH. Compared with the group of Gal / Gah (5.5), the group of GE / GA _ 5) had obvious protective effects on spray drying of lactic acid bacteria and storage of humidity at room temperature; compared with the group of GE5.5, the protective effect of group GE / GA _ 5) on spray drying of lactic acid bacteria and storage of humidity at room temperature was not significant; compared with group GE / GA _ (5.0), it had no significant protective effect on lactic acid bacteria spray drying and humidity storage at room temperature. The stability of GE / GA / GA / S5.5 group was significantly improved by increasing the pH value of complex condensation from 102.6 鹵5.9% to 131.3 鹵8.0%, and the storage stability of the bacteria was improved obviously in the group of GE / GA / GA / S 5.5, which increased the survival rate of bacteria during spray drying from 102.6 鹵5.9% to 131.3 鹵8.0%. The microcapsule was stored under 30oC ~ (11) RH for 56 days, the number of lactic acid bacteria decreased only 1.63 log.K3) the optimum conditions for preparing oil / Zein micron particles by Zein antisolvent method were optimized. The typical oils were medium-chain triglyceride (Zein) and palm oil (PO3). The embedding effect of oil / Zein micron particles on lactic acid bacteria and the structure and physicochemical properties of microcapsules were studied. Based on Zein antisolvent method, MCT/Zein micron particles with good stability and obvious shell core structure were prepared by using GA as o / W emulsifier and Zein micron particle aqueous dispersion agent. The LAB/MCT/Zein micron and LAB/PO/Zein micron particles prepared by antisolvent method have good morphology and good dispersibility. They are encapsulated by lactic acid bacteria, but the survival of lactic acid bacteria is low due to the presence of ethanol.
【學(xué)位授予單位】：湖北工業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TS201.3

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 馬鐵錚;趙宏亮;王靜;;復(fù)合凝聚法制備脂溶性食品配料微膠囊的壁材研究進(jìn)展[J];食品工業(yè)科技;2016年13期

2 陳婷;王玉華;蔡丹;鄭明珠;修琳;劉景圣;;益生菌微膠囊技術(shù)研究進(jìn)展[J];中國(guó)乳品工業(yè);2016年01期

3 肖軍霞;杜艷麗;李有棟;黃國(guó)清;;復(fù)凝聚反應(yīng)及其應(yīng)用研究進(jìn)展[J];食品安全質(zhì)量檢測(cè)學(xué)報(bào);2015年12期

4 許辰琪;袁芳;高彥祥;;玉米醇溶蛋白作為傳遞載體研究進(jìn)展[J];中國(guó)食品添加劑;2015年07期

5 顧豐穎;賀凡;弓威;寧吉英;高萍萍;王鋒;;生物活性成分載體——玉米醇溶蛋白的研究現(xiàn)狀[J];沈陽(yáng)農(nóng)業(yè)大學(xué)學(xué)報(bào);2014年06期

6 楊艷麗;何玉鳳;劉潔;王燕;王榮民;;基于玉米醇溶蛋白高分子材料的制備與應(yīng)用進(jìn)展[J];化學(xué)通報(bào);2014年08期

7 楊雷;仇丹;周逸奎;湯成科;;阿拉伯膠的結(jié)構(gòu)特征和乳化性能研究進(jìn)展[J];食品工業(yè)科技;2013年12期

8 雷欣宇;曾凡坤;康建平;黃靜;;乳酸菌的生理功能及其食用制劑制備技術(shù)進(jìn)展[J];食品與發(fā)酵科技;2012年02期

9 李芳;王全杰;侯立杰;;明膠微膠囊的應(yīng)用現(xiàn)狀與發(fā)展趨勢(shì)[J];中國(guó)皮革;2011年01期

10 Constantine Iosif Fotiadis;Christos Nikolaou Stoidis;Basileios Georgiou Spyropoulos;Eleftherios Dimitriou Zografos;;Role of probiotics,prebiotics and synbiotics in chemoprevention for colorectal cancer[J];World Journal of Gastroenterology;2008年42期

相關(guān)博士學(xué)位論文 前3條

1 劉歡;噴霧干燥法乳酸菌微膠囊的制備及其在仔豬體內(nèi)的應(yīng)用研究[D];江南大學(xué);2016年

2 王麗娟;玉米醇溶蛋白膠體顆粒的制備及應(yīng)用研究[D];華南理工大學(xué);2014年

3 呂怡;復(fù)合凝聚反應(yīng)制備茉莉香精微/納米膠囊及其機(jī)理研究[D];江南大學(xué);2012年

相關(guān)碩士學(xué)位論文 前4條

1 杜歌;谷胱甘肽的復(fù)合凝聚微膠囊化技術(shù)研究[D];江南大學(xué);2015年

2 王慶衛(wèi);內(nèi)源乳化法制備雙歧桿菌微膠囊的研究[D];江南大學(xué);2014年

3 李玉輝;甜菜果膠與明膠的復(fù)合凝聚及其在微膠囊中的應(yīng)用[D];湖北工業(yè)大學(xué);2013年

4 于樂(lè)軍;明膠/阿拉伯膠復(fù)凝聚微膠囊技術(shù)用于乳酸菌保護(hù)的研究[D];中國(guó)海洋大學(xué);2009年

，

本文編號(hào)：1793082