本文選題：金納米簇　切入點：有機無機復(fù)合納米材料　出處：《太原理工大學(xué)》2017年碩士論文

【摘要】：無機納米材料,尤其是金納米材料,由于具有獨特的表面等離子體效應(yīng)、光電性質(zhì)以及生物相容性等優(yōu)勢,在生物傳感器、靶向給藥、生物組織和細胞成像及動力學(xué)治療等領(lǐng)域有著極其廣闊的應(yīng)用前景。金納米材料通過表面工程化修飾可有效改善其發(fā)光性能,并賦予其表面拉曼增強成像以及載藥、治療等功能,實現(xiàn)生物納米探針診療一體化,從而引起了科研人員廣泛而深入的關(guān)注。在本論文中,我們利用不同氨基酸修飾金納米簇,再與其他有機功能分子通過化學(xué)鍵作用復(fù)合與組裝,實現(xiàn)了有機無機復(fù)合型多功能生物納米探針的制備,并成功應(yīng)用于生物成像中。在第二章中,我們利用谷胱甘肽(GSH)還原高氯金酸合成金納米簇,接著與β-環(huán)糊精(β-CD)通過酯化反應(yīng)得到化學(xué)鍵合的Au-GS@β-CD復(fù)合納米粒子。經(jīng)實驗測試表明,Au-GS@β-CD納米粒子(NPs)實現(xiàn)了紅光發(fā)射,且隨著濃度的增加,發(fā)射強度逐漸增強。細胞毒性實驗表明,Au-GS@β-CD NPs毒性低,生物相容性好。激光掃描共聚焦顯微鏡成像結(jié)果表明,Au-GS@β-CD NPs對胃癌細胞(MGC-803)具有選擇透過性,可進行特異性熒光標記。溫度響應(yīng)實驗結(jié)果表明,隨著溫度的升高,Au-GS和Au-GS@β-CD NPs熒光強度逐漸變?nèi)�。金屬離子檢測結(jié)果表明,Au-GS和Au-GS@β-CD NPs都對Pb2+、Al3+、Cu2+、Ag+和Fe3+作出了響應(yīng)。在第三章中,我們設(shè)計并合成了一系列磁性熒光納米粒子Fe3O4@Au,Fe3O4@Au@SiO2,Fe3O4@Au@β-CD;對其結(jié)構(gòu)和性能進行了詳細表征,這三種材料都實現(xiàn)了磁共振成像和熒光成像能力的結(jié)合,其中Fe3O4@Au@β-CD NPs呈現(xiàn)明顯的核殼結(jié)構(gòu),且水溶性要明顯優(yōu)于Fe3O4@Au和Fe3O4@Au@SiO2 NPs,同時最大熒光發(fā)射峰紅移,實現(xiàn)紅色熒光發(fā)射。隨著濃度的增加,三種磁性納米材料熒光發(fā)射強度均明顯增強。細胞毒性實驗表明,Fe3O4@Au,Fe3O4@Au@SiO2和Fe3O4@Au@β-CD NPs均具有較低的生物毒性,而Fe3O4@Au@β-CD NPs由于其高水溶性表現(xiàn)出良好的生物相容性。激光掃描共聚焦顯微鏡成像結(jié)果表明Fe3O4@Au@β-CD NPs對胃癌細胞(MGC-803)具有良好的選擇透過性,因此可以進行特異性標記。在第四章中,我們將鉑卟啉(Pt-TCPP)與金納米簇(Au-L-cys)通過酯化反應(yīng)進行化學(xué)鍵合生成Au-L-cys@Pt-TCPP有機無機復(fù)合納米粒子,該納米粒子兼具金屬卟啉和金納米簇的性能,其最大發(fā)射峰到達紅光區(qū)域。細胞毒性實驗表明,Au-L-cys@Pt-TCPP NPs具有較低的生物毒性和良好的生物相容性。此外,激光掃描共聚焦顯微鏡成像結(jié)果表明Au-L-cys@Pt-TCPP NPs對胃癌細胞(MGC-803)有較好的選擇透過性,可實現(xiàn)特異性紅光成像標記。同時對HeLa細胞實現(xiàn)了雙波段檢測功能:藍光波段(420-480 nm)和紅光波段(620-680 nm)。綜上所述,本論文基于對金納米簇和磁性熒光納米粒子的表面工程化修飾,實現(xiàn)了多功能高水溶性生物納米探針的合成,為癌細胞診療一體化試劑的研發(fā)提供了極具應(yīng)用前景的候選材料。
[Abstract]:Inorganic nanomaterials, especially gold nanomaterials, because of their unique surface plasma effect, optoelectronic properties and biocompatibility, are used in biosensor, targeted drug delivery, etc.Biological tissue and cell imaging and kinetic therapy have broad application prospects.Gold nanomaterials can effectively improve their luminescence performance by surface engineering modification, and endow them with the functions of surface Raman enhanced imaging, drug loading and therapy, so as to realize the integration of biological nano-probe diagnosis and treatment.Thus caused the scientific research personnel widespread and the thorough concern.In this thesis, we used different amino acids to modify gold nanoclusters, and then we combined and assembled with other organic functional molecules by chemical bond to achieve the preparation of organic-inorganic multifunctional biological nanoprobes.And successfully applied in biological imaging.In chapter 2, we use glutathione glutathione (GSH) to reduce gold nanoclusters with high chloro gold acid, and then we react with 尾 -cyclodextrin (尾 -CD) to obtain chemically bonded Au-GS@ 尾 -CD composite nanoparticles.The experimental results show that the red light emission is realized by Au-GS@ 尾 -CD nanoparticles (NPs), and the emission intensity increases with the increase of concentration.Cytotoxicity test showed that Au-GS @ 尾 -CD NPs had low toxicity and good biocompatibility.The results of laser scanning confocal microscopy showed that Au-GS @ 尾 -CD NPs had selective permeability to gastric cancer cell line MGC-803 and could be labeled with specific fluorescence.The results of temperature response experiments show that the fluorescence intensity of Au-GS and Au-GS@ 尾 -CD NPs weakens with the increase of temperature.The results of metal ion detection show that both Au-GS and Au-GS @ 尾 -CD NPs are responsive to Pb2 Al3 + Cu2 + Ag and Fe3.In Chapter 3, we have designed and synthesized a series of magnetic fluorescent nanoparticles Fe _ 3O _ 4O _ 4O _ (Fe3O _ 4) Au@ 尾 -CD.The structure and properties of these materials have been characterized in detail. The three materials have realized the combination of magnetic resonance imaging (MRI) and fluorescence imaging.Fe3O4 @ 尾 -CD NPs has obvious core-shell structure, and its water solubility is obviously superior to that of Fe3O4@Au and Fe3O4@Au@SiO2 NPs. Meanwhile, the maximum fluorescence emission peak shifts red to realize red fluorescence emission.With the increase of the concentration, the fluorescence emission intensity of the three kinds of magnetic nanomaterials increased obviously.The cytotoxicity tests showed that Fe3O4R, Fe3O4R, Fe3O4R, SiO2 and Fe3O4R @ 尾 -CD NPs had lower biotoxicity, while Fe3O4R Au@ 尾 -CD NPs showed good biocompatibility due to its high water solubility.The results of laser scanning confocal microscopy showed that Fe3O4R Au@ 尾 -CD NPs had a good selective permeability to gastric cancer cell line MGC-803, so it could be labeled specifically.In chapter 4, we chemically bond platinum porphyrin (Pt-TCPP) with gold nanoclusters Au-L-cyss to form Au-L-cys@Pt-TCPP organic-inorganic composite nanoparticles, which have the properties of both metalloporphyrin and gold nanoclusters.The maximum emission peak reaches the red region.Cytotoxicity test showed that Au-L-cysr Pt-TCPP NPs had low biotoxicity and good biocompatibility.In addition, the results of laser scanning confocal microscopy showed that Au-L-cys@Pt-TCPP NPs had a better selective transmittance to gastric cancer cell line MGC-803, and could be used as a specific red light imaging marker.At the same time, the HeLa cells were detected in two bands: blue band (420-480 nm) and red light band (620-680 nm).In conclusion, based on the surface engineering modification of gold nanoclusters and magnetic fluorescent nanoparticles, the synthesis of multifunctional and highly water-soluble biological nanoprobes has been achieved.It provides a promising candidate material for the research and development of cancer cell diagnosis and treatment integration reagent.
【學(xué)位授予單位】：太原理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：TB383.1;O657.3

【參考文獻】

相關(guān)期刊論文 前10條

1 林森;;大數(shù)據(jù)解讀癌癥[J];百科知識;2016年08期

2 姚翠萍;王萌萌;王晶;張鎮(zhèn)西;;基于納米金光學(xué)性質(zhì)的分子檢測與應(yīng)用[J];激光生物學(xué)報;2015年04期

3 李s，

本文編號：1713693