本文關(guān)鍵詞： 季銨鹽 鹵胺 納米金剛石 分散性 抗菌 再生性　出處：《南京理工大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：近年來,微生物威脅人類安全和健康成為社會(huì)關(guān)注的焦點(diǎn)�？咕牧献鳛橛行б种萍�(xì)菌感染的手段,其研發(fā)尤其是新型抗菌材料的研發(fā)不斷引起重視�？咕牧蠌V泛應(yīng)用于水凈化系統(tǒng)、醫(yī)療器械、食品包裝、食物保鮮、衛(wèi)生設(shè)施等領(lǐng)域內(nèi)。納米金剛石具有優(yōu)異的力學(xué)、光學(xué)、熱學(xué)性質(zhì),有廣闊的應(yīng)用前景,最近其在抗菌領(lǐng)域的研究得到關(guān)注。鹵胺化合物因具有良好的廣譜殺菌性、穩(wěn)定性和再生性,對人體和環(huán)境安全等優(yōu)點(diǎn)而受到關(guān)注,鹵胺通過釋放氧化態(tài)氯離子,破壞細(xì)胞基質(zhì),促使細(xì)菌死亡。季銨鹽類抗菌劑特別是高分子季銨鹽表面由于其帶有的豐富正電荷可以吸附到細(xì)菌表面并殺死細(xì)菌,研究表明季銨鹽基團(tuán)表面電荷可以改善納米粒子分散性能。本文以納米金剛石為載體通過兩種方法制備了兩種高分子季銨鹽型鹵胺改性的納米金剛石復(fù)合粒子,并對其化學(xué)組成、分散性能和抗菌能力等進(jìn)行了深入研究。具體研究內(nèi)容如下:1、聚陽離子型鹵胺改性的納米金剛石復(fù)合抗菌材料(PCN-NDs)。以納米金剛石為載體,用季銨化鹵胺高分子對其成功改性,制備了聚陽離子型鹵胺改性的納米金剛石顆粒。利用紅外光譜(FT-IR)、核磁共振氫譜(1HNMR)、透射電鏡(TEM)、熱重分析(TG)以及X-射線光電子能譜(XPS)等對納米顆粒的化學(xué)組成、分散性能等進(jìn)行表征分析。通過抗菌動(dòng)力學(xué)實(shí)驗(yàn)和最小抑菌濃度測試等手段來評(píng)價(jià)比較所制備抗菌材料的抗菌性能。實(shí)驗(yàn)結(jié)果表明:聚陽離子改性對納米粒子具有明顯的解團(tuán)聚效果,大幅提升了分散性能,可以將納米金剛石團(tuán)聚體分離成40-50nm。PCN-NDs抗菌納米顆粒在和細(xì)菌接觸15 min時(shí)間內(nèi)即可殺死100%的E.coli,5min內(nèi)殺死100%的S.aureus。此外還發(fā)現(xiàn)PCN-NDs具備良好的再生性和儲(chǔ)存穩(wěn)定性。2、鹵胺改性殼聚糖/納米金剛石復(fù)合抗菌材料(Cl-DMH-CTS-NDs)。以納米金剛石為載體,在納米金剛石表面組裝鹵胺改性殼聚糖,成功制備鹵胺改性殼聚糖/納米金剛石顆粒。利用紅外光譜(FT-IR)、核磁共振氫譜(1HNMR)透射電鏡(TEM)、熱重分析(TG)以及Zeta電位等對納米復(fù)合粒子的化學(xué)組成、分散性能等進(jìn)行表征分析。通過抗菌動(dòng)力學(xué)測試、最小抑菌濃度測試等手段來評(píng)價(jià)比較制備抗菌材料的抗菌性能。實(shí)驗(yàn)結(jié)果表明,改性方案有效的改善了納米金剛石的分散穩(wěn)定性;Cl-DMH-CTS-NDs抗菌納米顆粒在和細(xì)菌接觸30 min的時(shí)間內(nèi),即可殺死100%的E.coli和的S.aaureus。Cl-DMH-CTS-NDs具備良好的再生性,釋放活性氯后能夠通過簡單的氯化再生。
[Abstract]:In recent years, microbial threats to human safety and health have become the focus of attention of the community. Antimicrobial materials as an effective means of inhibiting bacterial infection, Antibacterial materials are widely used in water purification systems, medical devices, food packaging, food preservation, sanitary facilities and other fields. Nanocrystalline diamond has excellent mechanics, optics, etc. Its thermal properties have broad application prospects, and its recent research in the field of antimicrobial has attracted attention. Halogenamine compounds have attracted much attention because of their advantages of good broad-spectrum bactericidal properties, stability, reproducibility, and safety to human body and environment. Halamine kills bacteria by releasing oxidized chlorine ions, destroying the cell matrix and causing bacteria to die. Quaternary ammonium salts, especially macromolecular quaternary ammonium salts, can be adsorbed on the surface of bacteria and killed because of their rich positive charges. The results show that the surface charge of quaternary ammonium salt group can improve the dispersion of nanoparticles. In this paper, two kinds of high molecular quaternary ammonium salt modified nanocrystalline diamond composite particles were prepared by two methods using nanocrystalline diamond as the carrier. The chemical composition, dispersion and antimicrobial ability of nanocrystalline diamond were studied in detail as follows: 1, polycationic halogenamine modified nano-diamond composite antibacterial material PCN-NDs1.The nanocrystalline diamond was used as the carrier. The quaternary ammonium halogen amine polymer was successfully modified. Nano-diamond particles modified by polycationic halogenamine were prepared. The chemical composition of nanocrystalline diamond particles was studied by FTIR, 1H NMR, TEM, TG) and X- ray photoelectron spectroscopy (XPS). The antibacterial properties of the prepared antimicrobial materials were evaluated and compared by means of antibacterial kinetic test and minimal inhibitory concentration test. The experimental results showed that polycationic modification had good properties on nanoparticles. It has obvious effect of unreuniting. Greatly improved dispersion performance, Nanocrystalline diamond aggregates can be separated into 40-50nm.PCN-NDs antibacterial nanoparticles, which can kill 100% of E. coli and 100% S.aureus within 5 minutes after contact with bacteria for 15 min. In addition, it is also found that PCN-NDs has good regeneration and storage stability of 0.2Halogen modified. Chitosan / nanocrystalline diamond composite antibacterial material Cl-DMH-CTS-NDs0.Using nanocrystalline diamond as carrier, Halogenamine modified chitosan was assembled on the surface of nanocrystalline diamond. Halogenamine modified chitosan / nanocrystalline diamond particles were successfully prepared. The chemical composition of nano-composite particles was studied by FTIR, 1H NMR, TEM, TG) and Zeta potential. The antibacterial properties of the prepared antimicrobial materials were evaluated and compared by means of antibacterial kinetic test, minimal inhibitory concentration test and so on. The experimental results showed that, The modified method can effectively improve the dispersion stability of nanocrystalline diamond. Within 30 min of contact with bacteria, Cl-DMH-CTS-NDs can kill 100% E. coli and S.aaureus.Cl-DMH-CTS-NDs. After releasing active chlorine, it can be regenerated by simple chlorination.
【學(xué)位授予單位】：南京理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TB34

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