本文關(guān)鍵詞：費(fèi)約果香氣成分研究及生物活性初探　出處：《西南科技大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 費(fèi)約果 香氣 提取工藝 成分分析 抗氧化性 抑菌活性

【摘要】：為了對(duì)費(fèi)約果香氣成分有全面認(rèn)識(shí),文章采用頂空固相微萃取結(jié)合氣相色譜-質(zhì)譜(HS-SPME-GC-MS)對(duì)費(fèi)約果不同品種不同器官香氣成分進(jìn)行了分析,并對(duì)費(fèi)約果葉片精油生物活性進(jìn)行了初探。主要研究?jī)?nèi)容和結(jié)果如下:1、采用三種不同方法(索氏抽提法、超聲波輔助溶劑萃取法、頂空固相微萃取法)對(duì)費(fèi)約果葉片香氣進(jìn)行萃取,經(jīng)氣相色譜-質(zhì)譜(GC-MS)分析后發(fā)現(xiàn),三種方法分別能檢測(cè)到36、26和63種物質(zhì)。就種類而言,頂空固相微萃取法能得到最多的香氣成分。就性質(zhì)而言,頂空固相微萃取法直接頂空吸附進(jìn)樣,萃取的香氣組分最接近植物體散發(fā)的香氣。2、采用頂空固相微萃取法,以75μm CAR/PDMS萃取頭為吸附介質(zhì),設(shè)計(jì)L9(34)正交試驗(yàn)對(duì)萃取條件進(jìn)行優(yōu)化,以出峰個(gè)數(shù)和總峰面積為考察指標(biāo),進(jìn)行極差分析。得出最佳實(shí)驗(yàn)條件為:加樣量0.6 g,萃取溫度50℃,萃取時(shí)間50 min,解析時(shí)間6 min。在此條件下,可分離得到費(fèi)約果葉片中88種物質(zhì),鑒定出其中65種,占香氣總量的96.583%,優(yōu)于正交試驗(yàn)中任何一組,說(shuō)明選出的萃取條件切實(shí)可行,能最大程度反映香氣構(gòu)成。3、以“2”中優(yōu)化所得萃取條件,采用頂空固相微萃取法(HS-SPME)結(jié)合氣相色譜-質(zhì)譜(GC-MS),對(duì)費(fèi)約果不同品種不同器官香氣進(jìn)行萃取分析,共可在葉片中鑒定出114種香氣成分,全果中鑒定出87種香氣成分,花朵中鑒定出106種香氣成分,分析發(fā)現(xiàn),不同品種不同器官間香氣差異較大。4、采用溶劑萃取法提取費(fèi)約果葉片精油,分別對(duì)其進(jìn)行超氧陰離子自由基、羥基自由基、DPPH自由基清除實(shí)驗(yàn)和脂質(zhì)過(guò)氧化抑制實(shí)驗(yàn),以抗壞血酸為標(biāo)準(zhǔn)品,探究精油抗氧化活性;以食品殺菌中常用的目標(biāo)菌大腸桿菌和金黃色葡萄球菌為供試菌,探究精油抑菌活性。結(jié)果表明,費(fèi)約果葉片精油對(duì)超氧陰離子自由基、羥基自由基、DPPH自由基表現(xiàn)出了較強(qiáng)的清除能力,對(duì)脂質(zhì)過(guò)氧化也表現(xiàn)出了較強(qiáng)的抑制能力,且對(duì)DPPH自由基清除力和脂質(zhì)過(guò)氧化抑制力遠(yuǎn)遠(yuǎn)強(qiáng)于抗壞血酸標(biāo)準(zhǔn)品,對(duì)超氧陰離子自由基和羥基自由基的清除力略低于抗壞血酸標(biāo)準(zhǔn)品。同時(shí),費(fèi)約果葉片精油對(duì)大腸桿菌和金黃色葡萄球菌皆表現(xiàn)出了一定的抑制作用,且對(duì)大腸桿菌的抑制作用強(qiáng)于金黃色葡萄球菌。
[Abstract]:In order to have a comprehensive understanding of the aroma components of Feryogo. Headspace solid-phase microextraction (SPME) combined with gas chromatography-mass spectrometry (GC-MS) was used to analyze the aroma components of different varieties and organs of Feiyogu. The biological activity of essential oil from Feryogor leaves was studied. The main contents and results were as follows: 1. Three different methods (Soxhlet extraction and ultrasonic assisted solvent extraction) were used. Headspace solid-phase microextraction (SPME) was used to extract the aroma of Feyoguo leaves. After GC-MS analysis, it was found that 36% could be detected by the three methods respectively. 26 and 63 kinds of substances. Headspace solid phase microextraction method can obtain the most aroma components. In terms of properties, headspace solid phase microextraction method can directly adsorb samples in headspace. The aroma component of extraction is closest to that of plant. 2. Headspace solid phase microextraction (SPME) method with 75 渭 m CAR/PDMS head as adsorption medium was used. The extraction conditions were optimized by the orthogonal design of L9 ~ (34). The optimum experimental conditions were obtained as follows: the amount of sample was 0.6 g, and the total peak area and the number of peaks were taken as the index of investigation. The extraction temperature was 50 鈩，

本文編號(hào)：1439856