本文關(guān)鍵詞：功能化納米復(fù)合材料構(gòu)建的免疫傳感器用于腫瘤標(biāo)志物檢測(cè)　出處：《湖北民族學(xué)院》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 電化學(xué) 免疫傳感器 納米材料 腫瘤標(biāo)志物

【摘要】：金屬納米材料(金納米粒子、銀納米粒子、鉑納米粒子)和碳納米材料(氧化石墨烯,碳納米管)具有超強(qiáng)的導(dǎo)電性,良好的生物相容性以及巨大的比表面積等優(yōu)良的物理化學(xué)性能,被廣泛的應(yīng)用于免疫傳感器中。將這些納米材料經(jīng)功能化復(fù)合后形成的功能化納米復(fù)合材料,其各項(xiàng)性能都大大的增強(qiáng)。基于此本論文構(gòu)建了腫瘤標(biāo)志物檢測(cè)的新方法,其主要內(nèi)容包括以下五章:第一章:緒論。簡(jiǎn)要概述癌癥的發(fā)病機(jī)理及其檢測(cè)方法,免疫傳感器的分類及檢測(cè)原理,詳細(xì)介紹金屬納米材料和碳納米材料在免疫傳感器的應(yīng)用,最后介紹本論文的研究?jī)?nèi)容。第二章:實(shí)驗(yàn)基于納米鉑-單壁碳納米管-殼聚糖納米復(fù)合材料修飾玻碳電極,并通過納米鉑及富含羧基的單壁碳納米管將癌胚抗原抗體固定到電極表面制成了免疫傳感界面。選用癌胚抗原(CEA)作為分析模型,構(gòu)建一種簡(jiǎn)單靈敏無標(biāo)記的阻抗型免疫分析方法。在最優(yōu)化的條件下,CEA濃度在0.01~12 ng/m L范圍時(shí),阻抗值隨著CEA的濃度增加而增加并表現(xiàn)出良好的線性關(guān)系,檢測(cè)限為0.0042 ng/mL(S/N=3)。該免疫傳感器具有良好的穩(wěn)定性和選擇性,回收率在94.6%~108.6%。第三章:實(shí)驗(yàn)以性能優(yōu)良的十二面體金-銀核殼型納米晶體復(fù)合材料與前列腺特異性抗體結(jié)合制備免疫復(fù)合物為信號(hào)探針,金電極上電沉積一層銀納米花后與金納米/氧化石墨烯/殼聚糖混合碳晶膠納米材料復(fù)合制得的免疫傳感平臺(tái),采用夾心型免疫分析模式,建立了信號(hào)抑制型的前列腺特異性抗原免疫傳感器。其中銀納米花(AgNFs)作為氧化還原電子媒介體,并隨著PSA濃度的增加,免疫傳感器上形成的免疫復(fù)合物越多越多抑制銀的電化學(xué)響應(yīng),導(dǎo)致了DPV峰電流信號(hào)逐漸減小。在最優(yōu)化條件下,免疫傳感器對(duì)PSA檢測(cè)的范圍為1.0×10-5~20.0 ng/mL,檢測(cè)限可達(dá)到3.6×10-6 ng/mL(S/N=3)。第四章:實(shí)驗(yàn)采用功能化修飾的金納米棒捕獲甲胎蛋白抗體形成納米復(fù)合物,通過電沉積在金電極上的銀納米花將納米復(fù)合物修飾到電極表面,制得電化學(xué)免疫傳感器,構(gòu)建了一個(gè)簡(jiǎn)單靈敏無信號(hào)標(biāo)記的甲胎蛋白(AFP)檢測(cè)新方法。檢測(cè)范圍為0.001~20 ng/m L,檢測(cè)限為0.5 pg/m L(S/N=3),對(duì)臨床樣品的分析結(jié)果與商用電化學(xué)發(fā)光法一致。第五章:實(shí)驗(yàn)將制備的爆米花狀的金納米粒子作為分子標(biāo)記物同時(shí)捕獲甲胎蛋白抗體和辣根過氧化酶形成信標(biāo)探針。同時(shí)以氧化石墨烯-亞甲基藍(lán)-金納米粒子納米復(fù)合材料滴涂到玻碳電極表面上固定甲胎蛋白抗體構(gòu)建免疫傳感平臺(tái)。采用夾心型的免疫分析模式,建立了一種新型電化學(xué)免疫傳感分析方法用于檢測(cè)甲胎蛋白(AFP)。在優(yōu)化的實(shí)驗(yàn)條件下,該免疫傳感器對(duì)AFP檢測(cè)限為0.32 pg/m L(S/N=3),并能用于實(shí)際樣品的檢測(cè)中。
[Abstract]:Metal nanoparticles (gold nanoparticles, silver nanoparticles, platinum nanoparticles) and carbon nanomaterials (graphene oxide, carbon nanotubes) have super conductivity. Excellent physical and chemical properties such as good biocompatibility and large specific surface area are widely used in immunosensors. Based on this paper, a new method for detection of tumor markers has been constructed. The main contents include the following five chapters: chapter 1: introduction. A brief overview of the pathogenesis of cancer and its detection methods. The classification and detection principle of immunosensor, the application of metal nano-material and carbon nano-material in immunosensor are introduced in detail. Finally, the research content of this thesis is introduced. Chapter 2: the experiment is based on the modified glassy carbon electrode based on nano-platinum single-walled carbon nanotube-chitosan nanocomposites. The immunosensor interface was made by immobilization of carcinoembryonic antigen antibody to the electrode surface by platinum nanoparticles and carboxyl-rich single-walled carbon nanotubes. Carcinoembryonic antigen (CEA) was used as the analytical model. A simple, sensitive and unlabeled impedance immunoassay was constructed. Under the optimal conditions, the concentration of CEA was in the range of 0.01 ~ 12 ng/m / L. The impedance value increases with the concentration of CEA and shows a good linear relationship. The detection limit is 0.0042 ng / mL / S / N ~ (3 +). The immunosensor has good stability and selectivity. The recovery rate is 94.6 and 108.6. Chapter III:. In the experiment, the immune complex was prepared by combining 12 hedron gold-silver core-shell nanocrystalline composite with prostate specific antibody as signal probe. The immunosensor platform was prepared by electrodepositing a layer of silver nanosplast and mixed with gold nanocrystalline / graphene oxide / chitosan on the gold electrode. The sandwich immunoassay model was used. A signal suppressive prostate specific antigen immunosensor was established in which AgNFs was used as redox electronic medium and the concentration of Agns increased with the increase of PSA concentration. The more immune complexes formed on the immunosensor, the more inhibition of the electrochemical response of silver, which led to the gradual decrease of DPV peak current signal. The detection range of PSA by immunosensor was 1.0 脳 10 ~ (-5) ng/mL. The detection limit can reach 3.6 脳 10 ~ (-6) ng 路m ~ (-1) mg / m ~ (-1) S / N ~ (3). Chapter 4th: functional modified gold nanorods were used to capture alpha-fetoprotein antibodies to form nanocomplexes. An electrochemical immunosensor was prepared by modifying the nano-composite onto the surface of the electrode by electrodeposition of silver nanoparticles on the gold electrode. A simple and sensitive AFP method for detection of alpha-fetoprotein was constructed with a detection range of 0.001 ~ 20 ng/m / L. The detection limit was 0.5 pg/m L ~ (-1) S / N ~ (3). The results of analysis of clinical samples are consistent with those of commercial electrochemiluminescence. Chapter 5th:. The prepared popcorn gold nanoparticles were used as molecular markers to capture alpha-fetoprotein antibody and horseradish peroxidase simultaneously to form a beacon probe. The material was coated on the surface of glassy carbon electrode to immobilize AFP antibody to construct the immunosensor platform. The sandwich immunoassay model was used. A novel electrochemical immunosensor method was developed for the detection of AFPN. Under the optimized experimental conditions, a novel electrochemical immunosensor method was developed for the detection of AFP. The detection limit of the immunosensor for AFP is 0.32 pg/m / L ~ (-1) S / N ~ (3 +), and it can be used in the detection of real samples.
【學(xué)位授予單位】：湖北民族學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R730.4;O657.1

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