本文關(guān)鍵詞：具有美白和抑菌雙重功效的竹葉黃酮納米粒子　出處：《浙江大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 竹葉黃酮 納米粒子 美白 抑菌 化妝品功能因子

【摘要】：本文以竹葉黃酮(BLF)為載藥、芫根多糖(TP)為包材、ε-聚賴氨酸(ε-PL)為陽(yáng)離子橋,采用納米膠囊技術(shù)研制出竹葉黃酮納米粒子(BLF/TP/ε-PL),并圍繞美白功效和抑菌性能進(jìn)行評(píng)價(jià),以期得到天然、高效、安全的新型化妝品功能因子,并為竹葉黃酮和芫根多糖等天然產(chǎn)物在功能性化妝品中的高效應(yīng)用提供理論基礎(chǔ)與實(shí)踐指導(dǎo)。主要研究?jī)?nèi)容和結(jié)論如下:(1)以TP和ε-PL為材料,采用離子交聯(lián)法制備TP/ε-PL空載納米粒子。以粒徑和PDI分散指數(shù)為標(biāo)準(zhǔn),經(jīng)控制變量法對(duì)工藝條件進(jìn)行初步優(yōu)化。結(jié)果表明,當(dāng)TP的濃度為2～4 mg/mL、ε-PL的濃度為1～2 mg/mL、TP/ε-PL的質(zhì)量比為2:1時(shí),得到的TP/ε-PL納米粒子平均粒徑為292.5±2.17nm,PDI分散指數(shù)為0.169±0.02,呈類球形或球形,形態(tài)規(guī)則,均勻分散,粒徑分布集中,大小均一,可以作為負(fù)載竹葉黃酮的良好納米載體。(2)以BLF為包埋對(duì)象,制備出負(fù)載BLF的竹葉黃酮納米粒子。以粒徑分布和對(duì)BLF的包埋率為指標(biāo),經(jīng)單因素試驗(yàn)對(duì)制備工藝參數(shù)進(jìn)行優(yōu)化。結(jié)果表明:當(dāng)壁材配比(TP:ε-PL)為5:1,壁芯材比例為15:1,壁材濃度為3mg/mL,恒流泵流速為200 mL/h時(shí),制備得到的竹葉黃酮納米粒子大小均勻規(guī)則,平均粒徑在500～550nm之間,PDI分散指數(shù)為0.224±0.01,包埋率為45.27%。經(jīng)工藝條件重復(fù)性考察得到RSD2.0%,表明竹葉黃酮納米粒子的制備工藝可行性高,重復(fù)性好,操作簡(jiǎn)單。(3)以小鼠B-16黑素瘤細(xì)胞為體外評(píng)價(jià)模型,用業(yè)界公認(rèn)的美白因子熊果苷為陽(yáng)性對(duì)照,研究竹葉黃酮納米粒子對(duì)B16細(xì)胞體外培養(yǎng)過程中相關(guān)指標(biāo)的影響,從而對(duì)其美白功效進(jìn)行評(píng)價(jià)。結(jié)果表明:竹葉黃酮納米粒子對(duì)B16細(xì)胞內(nèi)酪氨酸酶活性、黑色素合成和細(xì)胞增殖均表現(xiàn)出濃度依賴的抑制作用,當(dāng)樣品的濃度為100μg/mL時(shí),抑制率分別達(dá)到69.68%、62.35%和59.14%,各項(xiàng)指標(biāo)均顯著優(yōu)于熊果苷(p0.05)。同時(shí),竹葉黃酮納米粒子對(duì)B16細(xì)胞增殖抑制的IC50值大于對(duì)其黑素生成抑制的IC50值,表明其實(shí)際應(yīng)用的安全性較高。(4)以此竹葉黃酮納米粒子(BLF/TP/ε-PL)為研究對(duì)象,以革蘭氏陰性菌大腸桿菌(Escherichia coli)和革蘭氏陽(yáng)性菌枯草芽孢桿菌(Bacillus subtilis)為供試菌種,采用牛津杯法,對(duì)其抑菌性能進(jìn)行分析測(cè)試,并與ε-PL的抑菌效果相比較。結(jié)果表明:竹葉黃酮納米粒子對(duì)大腸桿菌和枯草芽孢桿菌均有較強(qiáng)的抑制能力,最小抑菌濃度低于0.625mg/mL,且抑制效果呈現(xiàn)濃度依賴性;與同濃度的ε-PL相比,竹葉黃酮納米粒子的抑菌效果略為遜色。綜上所述,BLF/TP/ε-PL結(jié)構(gòu)的竹葉黃酮納米粒子成分天然,制備簡(jiǎn)單,性質(zhì)穩(wěn)定均一,同時(shí)具有較強(qiáng)的美白、抑菌功效及較高的使用安全性,具有作為可視同天然的化妝品功能因子(Cosmeceuticals)開發(fā)的潛力。
[Abstract]:In this paper, bamboo leaf flavonoids (BLF) as drug carrier, yuenkanin polysaccharide (TP) for package material, poly-L-lysine (-PL) as cation bridge, developed using nanocapsule technology of bamboo leaf flavonoids nanoparticles (BLF/TP/ e -PL), and evaluate the whitening effect around and antibacterial performance, in order to get natural, efficient and Safe Cosmetics functional factors, and to provide theoretical basis and practical guidance for the efficient application of bamboo leaf flavonoids and coriander root polysaccharide and other natural products in functional cosmetics. The main research contents and conclusions are as follows: (1) to TP and E -PL as material, preparing TP/ epsilon -PL nanoparticles with no-load ionic crosslinking method. The particle size and PDI dispersion index as the standard, the control variable method is used to optimize the process conditions. The preliminary results show that when the concentration of TP is 2 ~ 4 mg/mL, the concentration of epsilon -PL was 1 ~ 2 mg/mL, the quality of TP/ e -PL is 2:1, TP/ e -PL nanoparticles to the average particle The diameter is 292.5 + 2.17nm, PDI dispersion index was 0.169 + 0.02, spherical or spherical, regular shape, uniform dispersion, particle size distribution, uniform size, can be used as a good carrier load. Nano bamboo leaf flavonoids (2) with BLF as the embedded object, prepared nanoparticles. BLF flavonoids the particle size distribution and BLF encapsulation rate as the index, the single factor test of prepration process parameters were optimized. The results show that when the mass ratio of wall material (TP: e -PL) for 5:1, the core wall ratio of 15:1, concentration of wall material was 3mg/mL, the constant flow pump flow rate is 200 mL/h, system the prepared bamboo flavonoids nanoparticles with uniform size, average particle size between 500 ~ 550nm and PDI dispersion index is 0.224 + 0.01, the embedding rate is 45.27%. the process conditions of repetitive inspection by RSD2.0%, showed that bamboo leaf flavonoids nanoparticle preparation process feasibility, good repeatability, simple operation. (3) to small Mouse B-16 melanoma cells in vitro with a recognized evaluation model, whitening factor, arbutin as the positive control of bamboo leaf flavone related indicators in the process of nanoparticles on B16 cells cultured in vitro, and to evaluate its whitening effect. The results showed that the flavonoids nanoparticles in B16 cells on tyrosinase activity, melanin synthesis and cell proliferation showed a concentration dependent inhibitory effect, when the concentration of the sample is 100 g/mL, the inhibition rate reached 69.68%, 62.35% and 59.14%, the indicators were significantly better than those of Xiong Guogan (P0.05). At the same time, inhibition of flavonoids nanoparticles on the proliferation of B16 cells with IC50 values greater than the melanogenesis inhibiting IC50 value. The practical application of the high security. (4) the bamboo leaf flavonoids (BLF/TP/ e -PL) nanoparticles as the research object, the gram negative Escherichia coli (Escherichia and coli) The gram positive bacterium (Bacillus subtilis) were tested by Oxford cup method, and analyzed to test their antibacterial properties and antibacterial effect and E -PL is compared. The results showed that the inhibition ability of flavonoids nanoparticles strong against Escherichia coli and Bacillus subtilis had minimal inhibitory concentration lower than 0.625mg/mL, and the inhibitory effect in a concentration dependent manner; compared with the same concentration of -PL epsilon, inhibitory effect of Flavonoids from bamboo leaves were slightly inferior. In conclusion, the natural flavonoids composition of BLF/TP/ nanoparticles epsilon -PL structure, simple preparation, stable and uniform, with strong whitening, use safety and high antibacterial efficacy, as is visible with the natural cosmetics function factor (Cosmeceuticals) development potential.

【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TQ658

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 趙凡;馬駿;王濤;;竹葉黃酮的提取和利用前景研究[J];四川職業(yè)技術(shù)學(xué)院學(xué)報(bào);2006年01期

2 張凌云;;竹葉黃酮的研究與應(yīng)用進(jìn)展[J];現(xiàn)代食品科技;2006年02期

3 胡林福;;從竹葉中提取竹葉黃酮[J];技術(shù)與市場(chǎng);2007年11期

4 羅宇倩;郭輝;胡林福;施林巍;錢俊青;;竹葉黃酮的抗氧化活性研究[J];食品科技;2011年07期

5 孫長(zhǎng)花;張素華;張凌云;;竹葉黃酮的生理活性與應(yīng)用研究[J];四川食品與發(fā)酵;2006年01期

6 ;待開發(fā)的竹葉黃酮[J];技術(shù)與市場(chǎng);2006年10期

7 陳偉;陳惠;劉曉燕;徐宏楠;;竹葉黃酮的提取和測(cè)定研究進(jìn)展[J];中國(guó)食品添加劑;2007年04期

8 陶文亮;吳鈺娟;;竹葉黃酮提取分析方法的研究及其新進(jìn)展[J];貴州化工;2009年03期

9 游輝;孫愛東;唐玲;潘娜;陳健;;竹葉黃酮純化中吸附劑的優(yōu)選及解析特性研究[J];食品科學(xué);2010年08期

10 陳海光;于立梅;黃東雨;;竹葉黃酮的提取工藝及抑菌作用研究[J];仲愷農(nóng)業(yè)工程學(xué)院學(xué)報(bào);2010年03期

相關(guān)會(huì)議論文 前3條

1 孫國(guó)金;何潮洪;;超聲對(duì)竹葉黃酮浸取的影響[A];第三屆全國(guó)傳質(zhì)與分離工程學(xué)術(shù)會(huì)議論文集[C];2002年

2 呂錦芳;寧康健;薛艷梅;馬軍;朱前明;;竹葉黃酮對(duì)小鼠肝臟和肌肉中部分礦物質(zhì)元素含量的影響[A];全國(guó)動(dòng)物生理生化第十二次學(xué)術(shù)交流會(huì)論文摘要匯編[C];2012年

3 潘蘭英;鄭雅鶯;王向軍;錢永常;黃瑜秋;;竹葉黃酮對(duì)Caco-2細(xì)胞及其中P-糖蛋白的作用[A];2013全國(guó)中藥與天然藥物高峰論壇暨第十三屆全國(guó)中藥和天然藥物學(xué)術(shù)研討會(huì)論文集[C];2013年

相關(guān)重要報(bào)紙文章 前2條

1 本報(bào)記者　 黃平 通訊員　 葉福明 路平;研究所設(shè)在葡萄園里[N];經(jīng)濟(jì)日?qǐng)?bào);2006年

2 本報(bào)記者 薛志偉邋黃平;依靠科技創(chuàng)新發(fā)展竹產(chǎn)業(yè)[N];經(jīng)濟(jì)日?qǐng)?bào);2008年

相關(guān)博士學(xué)位論文 前3條

1 舒剛;竹葉黃酮對(duì)肉雞的生物效應(yīng)及作用機(jī)理研究[D];四川農(nóng)業(yè)大學(xué);2015年

2 焦晶晶;竹葉特征性黃酮類化合物研究[D];浙江大學(xué);2008年

3 呂兆林;竹葉黃酮和揮發(fā)油的制備及生物活性的研究[D];北京林業(yè)大學(xué);2009年

相關(guān)碩士學(xué)位論文 前10條

1 張盛;竹葉黃酮的抗炎作用及物質(zhì)基礎(chǔ)研究[D];湖北中醫(yī)藥大學(xué);2016年

2 王楠;具有美白和抑菌雙重功效的竹葉黃酮納米粒子[D];浙江大學(xué);2017年

3 李棟;竹葉黃酮的提取、純化、鑒定及其抗氧化和抑菌活性研究[D];華中農(nóng)業(yè)大學(xué);2008年

4 劉，

本文編號(hào)：1358054