本文關鍵詞：關于CCDC-55調(diào)節(jié)擬南芥鈣營養(yǎng)的生理和分子機制的初步探究　出處：《內(nèi)蒙古大學》2017年碩士論文　論文類型：學位論文

  更多相關文章： CCDC-55 Ca~(2+) 表型分析 ICP 亞細胞定位

【摘要】：在植物中,Ca2+作為第二信使,不僅參與對生物脅迫和非生物脅迫的應答,還參與細胞分裂、生長、轉(zhuǎn)錄調(diào)節(jié)等眾多進程;此外作為營養(yǎng)物質(zhì)Ca是細胞壁的組成成分。CCDC-55是包含螺旋卷曲結(jié)構(gòu)域的蛋白55的簡稱,此蛋白功能未知。對擬南芥CCDC-55(AT2G27280)基因缺失突變體進行Ca2+濃度梯度的表型分析,發(fā)現(xiàn)突變體具有低鈣敏感表型。鈣離子是二價陽離子,ccdc-55對其他植物必需的二價陽離子如鎂、鐵、鋅、錳這四種礦質(zhì)元素的如何響應不得而知,于是對其進行了 Mg2+、Fe2+、Zn2+、Mn2+濃度梯度的表型分析,發(fā)現(xiàn)突變體在低鐵、高鋅的培養(yǎng)基上與野生型擬南芥相比具有明顯差異,但對突變體進行元素含量的分析結(jié)果表明,在植物體內(nèi),突變體鈣、鐵、鋅含量與野生型植物并沒有顯著性差異,也就是說明該基因不參與這三種元素的儲存。亞細胞定位結(jié)果顯示CCDC-55定位于細胞核中,啟動子活性分析結(jié)果表明CCDC-55在植物幼嫩的組織中表達。這說明CCDC-55有可能與細胞中的其他蛋白質(zhì)相互作用調(diào)控植物的某些生物學過程,而在這些過程中Ca2+、Fe2+、Zn2+不可或缺。
[Abstract]:In plants, Ca 2 2, as the second messenger, not only participates in the response to biological stress and abiotic stress, but also participates in many processes, such as cell division, growth, transcription regulation and so on. In addition, as a nutrient, Ca is a component of cell wall. CCDC-55 is the abbreviation of protein 55 containing helical crimp domain. The function of this protein is unknown. The Ca2 concentration gradient phenotypic analysis of Arabidopsis thaliana CCDC-55 AT2G27280) gene deletion mutant was carried out. It was found that the mutant has a low calcium sensitive phenotype. It is not known how calcium ions are the bivalent cations necessary for other plants such as magnesium, iron, zinc and manganese. The phenotypic analysis of the concentration gradient of Mg2 Fe _ 2 and Zn _ 2O _ 2 mn _ 2 showed that the mutant had significant difference from that of wild type Arabidopsis thaliana on low iron and high zinc medium. However, the results of element analysis showed that there was no significant difference between the contents of Ca, Fe and Zn in plants and wild type plants. This means that the gene does not participate in the storage of these three elements. Subcellular localization results show that CCDC-55 is located in the nucleus. The results of promoter activity analysis showed that CCDC-55 was expressed in young tissues of plants, which suggested that CCDC-55 might interact with other proteins in cells to regulate some biological processes in plants. In these processes, Ca2 Fe _ 2 O _ 2 Zn _ 2 is indispensable.
【學位授予單位】：內(nèi)蒙古大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：Q945;Q943.2

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1 陳穎慧;關于CCDC-55調(diào)節(jié)擬南芥鈣營養(yǎng)的生理和分子機制的初步探究[D];內(nèi)蒙古大學;2017年

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