本文關(guān)鍵詞：煤炭地下氣化廢水中苯酚的微生物降解研究　出處：《河南理工大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 煤炭地下氣化 地下水污染 苯酚 細(xì)菌 降解

【摘要】：煤炭地下氣化直接在地下將煤炭轉(zhuǎn)化成氣體燃料或原料,被譽(yù)為“綠色”開采技術(shù),但氣化過程中產(chǎn)生的有機(jī)污染物可能對地下水造成污染,影響其推廣應(yīng)用。有機(jī)污染物中尤以酚類化合物含量最高,主要為苯酚、甲酚、二甲酚等。酚類化合物為原生質(zhì)毒物,對所有生物均會產(chǎn)生一定毒害作用,我國把苯酚列為中國環(huán)境優(yōu)先控制污染物,因此對酚類化合物的脫除應(yīng)是煤炭地下氣化地下水有機(jī)污染物去除的重點。本文以活性污泥為菌源,以苯酚為唯一碳源篩選得到兩株苯酚優(yōu)勢降解菌:JC和WX,經(jīng)生理生化試驗和16SrDNA鑒定,JC為綠膿假單胞菌,WX為無色桿菌�？疾彀l(fā)現(xiàn)JC與WX菌最適生長環(huán)境為:溫度30~40℃,pH 7,搖床轉(zhuǎn)速120r/min,接種量7~16%。在苯酚濃度800mg/L,搖床轉(zhuǎn)速120r/min,溫度40℃的環(huán)境中,40~48h內(nèi)細(xì)菌生長繁殖最快,細(xì)菌活性也最高。分別利用管式爐及自行搭建的地下氣化模擬系統(tǒng),以褐煤為原料進(jìn)行水蒸氣氣化模擬試驗,收集煤氣洗滌水。用JC及WX菌對管式爐煤氣洗滌水原水TOC濃度為570mg/L及稀釋后TOC分別為375.07mg/L、211.94mg/L的廢水進(jìn)行降解處理,均具有良好降解效果,TOC為211.94mg/L的廢水經(jīng)60h降解處理后,TOC含量分別下降到64.427mg/L、62.944mg/L,TOC降解率分別為69.6%、70.3%。對煤炭地下氣化模擬廢水,將TOC從1089.19 mg/L稀釋到175.07 mg/L,經(jīng)JC及WX菌處理96h,廢水中TOC分別降低到42.01 mg/L、47.44 mg/L;使用美國安捷倫氣相色譜與質(zhì)譜聯(lián)用儀(HP 7890/5975)測定廢水中有機(jī)污染物組分及含量,經(jīng)細(xì)菌降解后廢水有機(jī)污染物中含量最高的苯酚、鄰甲苯酚、間甲苯酚含量為0,其他酚類化合物含量明顯降低,降解后廢水中出現(xiàn)烷烴類、烯烴類等小分子鏈烴化合物,可能細(xì)菌對苯酚等有機(jī)物的降解是逐步進(jìn)行的,先將大分子物質(zhì)分解為小分子,再進(jìn)行小分子有機(jī)物的降解。
[Abstract]:Underground gasification of coal directly converts coal into gas fuel or raw material, which is called "green" mining technology, but organic pollutants produced in gasification process may cause pollution to groundwater. The organic pollutants are mainly phenol, cresol, dimethylphenol and so on. Phenolic compounds are protoplast poisons, which have a certain toxic effect on all organisms. In China, phenol is listed as a priority pollutant for environmental control in China, so the removal of phenolic compounds should be the focus of removal of organic pollutants from underground coal gasification groundwater. In this paper, activated sludge is used as the source of bacteria. Using phenol as the sole carbon source, two strains of phenol-degrading bacteria were isolated and identified as Pseudomonas aeruginosa by physiological and biochemical tests and 16s rDNA. The optimum growth environment of JC and WX was as follows: temperature 30 ~ 40 鈩，

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