本文關鍵詞：小型化流式細胞儀的研制　出處：《浙江大學》2017年碩士論文　論文類型：學位論文

  更多相關文章： 小型化 流式細胞儀 激光誘導熒光 3D流體力學聚焦

【摘要】：流式細胞儀是一種用于單個細胞多參數(shù)快速定量分析和分選的先進儀器,在基礎科學研究與臨床診斷中發(fā)揮著重要作用。然而,傳統(tǒng)流式細胞儀體積龐大,結(jié)構(gòu)精密,操作復雜,主要面向?qū)I(yè)的科研型分析需求,并不適合場所流動性強的應用型分析需求。因此,基于微流控技術的流式細胞儀的小型化、集成化、便攜化研究正逐漸成為該領域發(fā)展的一大主流方向。本論文主要包括以下三個章節(jié)的內(nèi)容：第一章為文獻綜述,對流式細胞儀的原理概念、歷史發(fā)展、結(jié)構(gòu)組成等方面進行了全面的概括。進而,針對流式細胞儀中流體操控的核心——流體聚焦技術進行了具體討論。最后,分類介紹了當前流式細胞儀在各領域中的廣泛應用。第二章介紹了一臺小型化單熒光流式細胞儀的研制工作。該儀器基于共焦型激光誘導熒光檢測結(jié)構(gòu),以450 nm激光二極管為光源,實現(xiàn)前向散射光,側(cè)向散射光以及綠色熒光的同步檢測。利用3D打印技術設計制作了儀器的支撐結(jié)構(gòu),并以模塊化的方式將各部分組合在一起,整體尺寸僅為26×6×12 cm3。儀器中使用了多個微型平移臺結(jié)構(gòu),使部分模塊可以實現(xiàn)小范圍的微調(diào),以獲得最佳檢測效果。液流部分采用十字套管結(jié)構(gòu),通過傳統(tǒng)的流體力學聚焦模式實現(xiàn)對樣品流的3D聚焦。在本章中,我們利用標準尺寸的綠色熒光微球?qū)x器檢測性能進行了表征,并將其用于抗癌藥物誘導的癌細胞凋亡檢測,獲得較理想的結(jié)果。第三章在第二章工作的基礎上,初步進行了可實現(xiàn)多熒光檢測的小型化流式細胞儀的研制工作。儀器采用共焦光路作為熒光檢測結(jié)構(gòu),目標實現(xiàn)最多4色熒光,6參數(shù)的同步檢測功能。目前,我們完成了液流部分結(jié)構(gòu)的優(yōu)化以及對側(cè)向散射光和熒光檢測光路中透鏡位置的協(xié)調(diào)和優(yōu)化。通過對套管結(jié)構(gòu)中各管路位置進行調(diào)整,獲得了更加穩(wěn)定的且位置固定的3D鞘流。
[Abstract]:Flow cytometry is a kind of advanced instrument for rapid quantitative analysis of multi parameter and single cell sorting, plays an important role in basic scientific research and clinical diagnosis. However, the traditional flow cytometry in large volume, precise structure, complex operation, mainly for professional research demand analysis, is not suitable for application places the analysis of liquidity demand. Therefore, miniaturization, flow cytometry microfluidic technology based on integrated research, portable and has gradually become a mainstream direction of the development of this field. This paper mainly includes the following three chapters: the first chapter is the literature review, the concept of flow cytometry principle. The history of the development, structure composition were summarized. Then, aiming at the core flow cytometry in the control of fluid focusing technique were discussed in detail. Finally, the classification is introduced when Before the application of flow cytometry in various fields. The second chapter introduces the development of a miniature single fluorescence flow cytometry. The instrument structure of confocal laser induced fluorescence detection based on 450 nm laser diode as the light source, the light scattering, scattering light green fluorescence and synchronous detection the supporting structure of the instrument. Making use of 3D printing technology design, and in a modular way put all the pieces together, the overall size is only a plurality of micro displacement platform structure using 26 x 6 x 12 cm3. instruments, the module can achieve a small range of fine-tuning, to get the best result. The liquid flow part of the cross structure of casing, sample flow 3D focus by hydrodynamic focusing the traditional model. In this chapter, we use the green fluorescent microspheres standard size on detection performance were investigated, and For the detection of cancer cell apoptosis induced by anticancer drugs, obtained the ideal result. The third chapter in the second chapter, the preliminary research work can realize the miniaturization of multi fluorescence detection by flow cytometry. The instrument adopts the confocal optical path as fluorescence detection structure, the realization of the goal of a maximum of 4 color fluorescence 6, synchronous detection function parameters. At present, we have completed the flow optimization part of structure and the lateral scattering light and fluorescence detection of the position of the lens in the path of coordination and optimization. By adjusting the position of each pipeline casing structure, the more stable and fixed position of 3D sheath flow.

【學位授予單位】：浙江大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：Q-337

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相關期刊論文 前1條

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本文編號：1386691