發(fā)布時(shí)間：2018-01-06 06:27

  本文關(guān)鍵詞：小型化流式細(xì)胞儀的研制　出處：《浙江大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 小型化 流式細(xì)胞儀 激光誘導(dǎo)熒光 3D流體力學(xué)聚焦

【摘要】：流式細(xì)胞儀是一種用于單個(gè)細(xì)胞多參數(shù)快速定量分析和分選的先進(jìn)儀器,在基礎(chǔ)科學(xué)研究與臨床診斷中發(fā)揮著重要作用。然而,傳統(tǒng)流式細(xì)胞儀體積龐大,結(jié)構(gòu)精密,操作復(fù)雜,主要面向?qū)I(yè)的科研型分析需求,并不適合場(chǎng)所流動(dòng)性強(qiáng)的應(yīng)用型分析需求。因此,基于微流控技術(shù)的流式細(xì)胞儀的小型化、集成化、便攜化研究正逐漸成為該領(lǐng)域發(fā)展的一大主流方向。本論文主要包括以下三個(gè)章節(jié)的內(nèi)容：第一章為文獻(xiàn)綜述,對(duì)流式細(xì)胞儀的原理概念、歷史發(fā)展、結(jié)構(gòu)組成等方面進(jìn)行了全面的概括。進(jìn)而,針對(duì)流式細(xì)胞儀中流體操控的核心——流體聚焦技術(shù)進(jìn)行了具體討論。最后,分類介紹了當(dāng)前流式細(xì)胞儀在各領(lǐng)域中的廣泛應(yīng)用。第二章介紹了一臺(tái)小型化單熒光流式細(xì)胞儀的研制工作。該儀器基于共焦型激光誘導(dǎo)熒光檢測(cè)結(jié)構(gòu),以450 nm激光二極管為光源,實(shí)現(xiàn)前向散射光,側(cè)向散射光以及綠色熒光的同步檢測(cè)。利用3D打印技術(shù)設(shè)計(jì)制作了儀器的支撐結(jié)構(gòu),并以模塊化的方式將各部分組合在一起,整體尺寸僅為26×6×12 cm3。儀器中使用了多個(gè)微型平移臺(tái)結(jié)構(gòu),使部分模塊可以實(shí)現(xiàn)小范圍的微調(diào),以獲得最佳檢測(cè)效果。液流部分采用十字套管結(jié)構(gòu),通過(guò)傳統(tǒng)的流體力學(xué)聚焦模式實(shí)現(xiàn)對(duì)樣品流的3D聚焦。在本章中,我們利用標(biāo)準(zhǔn)尺寸的綠色熒光微球?qū)x器檢測(cè)性能進(jìn)行了表征,并將其用于抗癌藥物誘導(dǎo)的癌細(xì)胞凋亡檢測(cè),獲得較理想的結(jié)果。第三章在第二章工作的基礎(chǔ)上,初步進(jìn)行了可實(shí)現(xiàn)多熒光檢測(cè)的小型化流式細(xì)胞儀的研制工作。儀器采用共焦光路作為熒光檢測(cè)結(jié)構(gòu),目標(biāo)實(shí)現(xiàn)最多4色熒光,6參數(shù)的同步檢測(cè)功能。目前,我們完成了液流部分結(jié)構(gòu)的優(yōu)化以及對(duì)側(cè)向散射光和熒光檢測(cè)光路中透鏡位置的協(xié)調(diào)和優(yōu)化。通過(guò)對(duì)套管結(jié)構(gòu)中各管路位置進(jìn)行調(diào)整,獲得了更加穩(wěn)定的且位置固定的3D鞘流。
[Abstract]:Flow cytometry is a kind of advanced instrument for rapid quantitative analysis of multi parameter and single cell sorting, plays an important role in basic scientific research and clinical diagnosis. However, the traditional flow cytometry in large volume, precise structure, complex operation, mainly for professional research demand analysis, is not suitable for application places the analysis of liquidity demand. Therefore, miniaturization, flow cytometry microfluidic technology based on integrated research, portable and has gradually become a mainstream direction of the development of this field. This paper mainly includes the following three chapters: the first chapter is the literature review, the concept of flow cytometry principle. The history of the development, structure composition were summarized. Then, aiming at the core flow cytometry in the control of fluid focusing technique were discussed in detail. Finally, the classification is introduced when Before the application of flow cytometry in various fields. The second chapter introduces the development of a miniature single fluorescence flow cytometry. The instrument structure of confocal laser induced fluorescence detection based on 450 nm laser diode as the light source, the light scattering, scattering light green fluorescence and synchronous detection the supporting structure of the instrument. Making use of 3D printing technology design, and in a modular way put all the pieces together, the overall size is only a plurality of micro displacement platform structure using 26 x 6 x 12 cm3. instruments, the module can achieve a small range of fine-tuning, to get the best result. The liquid flow part of the cross structure of casing, sample flow 3D focus by hydrodynamic focusing the traditional model. In this chapter, we use the green fluorescent microspheres standard size on detection performance were investigated, and For the detection of cancer cell apoptosis induced by anticancer drugs, obtained the ideal result. The third chapter in the second chapter, the preliminary research work can realize the miniaturization of multi fluorescence detection by flow cytometry. The instrument adopts the confocal optical path as fluorescence detection structure, the realization of the goal of a maximum of 4 color fluorescence 6, synchronous detection function parameters. At present, we have completed the flow optimization part of structure and the lateral scattering light and fluorescence detection of the position of the lens in the path of coordination and optimization. By adjusting the position of each pipeline casing structure, the more stable and fixed position of 3D sheath flow.

【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：Q-337

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 王企鯤;孫仁;;管流中顆�！皯T性聚集”現(xiàn)象的研究進(jìn)展及其在微流動(dòng)中的應(yīng)用[J];力學(xué)進(jìn)展;2012年06期

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本文編號(hào)：1386691