本文關(guān)鍵詞：脫落酸對大鼠學習記憶的影響及可能機理　出處：《合肥工業(yè)大學》2017年碩士論文　論文類型：學位論文

  更多相關(guān)文章： ABA 樹突棘 樹突棘密度 NDR1/2激酶

【摘要】：脫落酸(ABA)作為關(guān)鍵的植物激素,廣泛存在于植物體內(nèi),現(xiàn)研究發(fā)現(xiàn)ABA也存在于動物(包括人)組織中,且具有多種生物活性。ABA作為類胡蘿卜素的直接衍生物,和視黃酸(RA)具有相似的分子結(jié)構(gòu),研究表明RA可以明顯改善嚙齒動物的空間記憶能力,但ABA對空間學習與記憶的影響及其分子機制尚不明確。目的:探究ABA給藥對成年SD大鼠空間記憶的影響;探究ABA給藥對SD大鼠海馬樹突棘密度及形態(tài)的影響,并從NMDA受體、Arc蛋白以及NDR1/2激酶途徑來探究其可能的分子機制。方法:1.ABA給藥實驗:ABA給藥劑量分別為5 mg/kg/day、25 mg/kg/day、50 mg/kg/day,給藥方式為腹腔注射,給藥時間為SD大鼠出生第七天開始一直到第八周進行水迷宮實驗的前一天為止;2.Golgi-cox染色:觀察ABA給藥后海馬神經(jīng)細胞的樹突棘形態(tài),通過Western blot及熒光定量PCR檢測NMDA受體、Arc蛋白以及NDR1/2激酶的蛋白表達。結(jié)果:1.行為學測試發(fā)現(xiàn),中劑量ABA增加了大鼠在目標象限中花費的持續(xù)時間和在Morris水迷宮(MWM)的探測測試中進入的頻率,并減少了于目標象限的等待時間。2.ABA能引起樹突棘結(jié)構(gòu)的顯著變化,表現(xiàn)為增加樹突棘的形態(tài)復雜性、樹突棘密度和成熟蘑菇狀樹突棘數(shù)目的增加。3.此外,ABA對NMDA受體R1、R2及Arc蛋白的表達無顯著影響,進一步研究發(fā)現(xiàn),ABA能顯著提高NDR1/2蛋白及下游基因Rabin3的表達量;離體的原代海馬神經(jīng)元轉(zhuǎn)染NDR1/2 shRNA,研究發(fā)現(xiàn)NDR下游基因Rabin3表達的顯著降低,即進一步驗證了NDR1/2激酶途徑參與了ABA對大鼠海馬區(qū)神經(jīng)元成熟過程的調(diào)節(jié)。結(jié)論:ABA通過NDR1/2激酶途徑調(diào)節(jié)大鼠海馬區(qū)神經(jīng)元樹突棘成熟過程,從而增強SD大鼠的學習記憶能力。
[Abstract]:Abscisic acid abscisic acid (Aba), as a key plant hormone, widely exists in plants. Now it has been found that ABA also exists in animal (including human) tissues. ABA as a direct derivative of carotenoid and retinoic acid (RAA) has similar molecular structure. Studies show that RA can significantly improve the spatial memory ability of rodents. But the effect of ABA on spatial learning and memory and its molecular mechanism are not clear. Objective: to explore the effect of ABA administration on spatial memory in adult SD rats. To investigate the effect of ABA administration on the density and morphology of hippocampal dendritic spine in SD rats, and to investigate the effects of NMDA receptor. Arc protein and NDR1/2 kinase pathway were used to explore its possible molecular mechanism. Methods: 1. The dosages of Arc protein and NDR1/2 kinase were 5 mg/kg/day respectively. 25 mg / kg / kg / day 50 mg / kg / kg / day, administered by intraperitoneal injection. The time of administration was from 7th days after birth to the day before the water maze test at 8th weeks. 2. Golgi-cox staining: the morphology of dendritic spine of hippocampal neurons was observed after ABA administration, and NMDA receptor was detected by Western blot and fluorescence quantitative PCR. Arc protein and NDR1/2 kinase protein expression. Results: 1. Behavioral test found. Medium-dose ABA increased the duration spent in the target quadrant and the frequency of entry in the Morris water maze (MWM) detection test. And reduced the waiting time in the target quadrant. 2. ABA can cause significant changes in the structure of dendritic spine, as shown by increasing the morphological complexity of dendritic spine. The density of dendritic spine and the number of mature mushroom dendritic spine increased .3.In addition, there was no significant effect of NMDA on the expression of R1R ~ 2 and Arc protein. ABA could significantly increase the expression of NDR1/2 protein and downstream gene Rabin3. The primary hippocampal neurons were transfected with NDR1/2 shRNAs in vitro. It was found that the Rabin3 expression of NDR downstream gene was significantly decreased. It is further verified that the NDR1/2 kinase pathway is involved in the regulation of ABA on the maturation of hippocampal neurons in rats. Conclusion:. ABA regulates the maturation of dendritic spine in rat hippocampal neurons through NDR1/2 kinase pathway. So as to enhance the learning and memory ability of SD rats.
【學位授予單位】：合肥工業(yè)大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：Q42

【參考文獻】

相關(guān)期刊論文 前3條

1 柴繼俠;王元元;李徽徽;賀文欣;鄒維艷;周艷梅;胡小冬;柴強;;突觸后致密蛋白95(PSD95)和突觸小泡蛋白在神經(jīng)元成熟過程中的分布[J];細胞與分子免疫學雜志;2016年12期

2 劉鑫;黃興;徐智云;楊德琴;;視黃酸信號通路調(diào)控咽弓神經(jīng)嵴影響斑馬魚牙齒發(fā)育的研究[J];華西口腔醫(yī)學雜志;2016年02期

3 侯學瑛;;用氣相色譜測定植物激素脫落酸[J];分析儀器;1986年03期

，

本文編號：1386116