當(dāng)今社會(huì),工業(yè)發(fā)展和人類活動(dòng)導(dǎo)致大量的土地受重金屬污染。農(nóng)業(yè)區(qū)過(guò)多的重金屬,例如,鉛(Pb)、鉻(Cr)、鋅(Zn)、鎘(Cd)、銅(Cu)和鎳(Ni)等嚴(yán)重威脅食品安全和公共健康。為了阻止人類和動(dòng)物遭受毒害,消除或者修復(fù)土壤重金屬污染是迫切需要的。鎘是一種有毒且非必須的重金屬,很容易被植物吸收并積累在農(nóng)產(chǎn)品中。目前,有很多防止、消除和修復(fù)環(huán)境中污染物負(fù)面效應(yīng)的方法和過(guò)程,但是對(duì)于此目的的應(yīng)用很難實(shí)施或者根本無(wú)法實(shí)施。因此,所有的研究應(yīng)考慮利用生物因素來(lái)處理和修復(fù)環(huán)境。植物修復(fù)被定義為利用植物消除環(huán)境中的污染物或者減少它們的危害。這個(gè)過(guò)程在不對(duì)環(huán)境造成干擾的同時(shí)還有一定的經(jīng)濟(jì)效益。高粱(Sorghum bicolor L.)是一種惠民的有多重用途的農(nóng)作物,在農(nóng)業(yè)生態(tài)系統(tǒng)中可以作為糧食、飼料、纖維和燃料。甜高粱包含了高粱的不同的栽培品種的有點(diǎn),且莖中儲(chǔ)存大量的蔗糖以及有很大的生物量,因此可以作為生物燃料,而糖含量確定了甜高粱作為生物燃料的潛力。甜高粱多汁的莖通常含有大約16-18%的可發(fā)酵糖和大量的無(wú)結(jié)構(gòu)性糖類。為了初步評(píng)估甜高粱植物修復(fù)的潛力,以前的研究已對(duì)重金屬(Cd,Pb,Zn,C...

【文章頁(yè)數(shù)】：149 頁(yè)

【學(xué)位級(jí)別】：博士

【文章目錄】：
摘要
ABSTRACT
CHAPTER I LITERATURE REVIEW
    1.1 Heavy metals in soils and plants
        1.1.1 Contaminated heavy metal soil
        1.1.2 Cadmium in the soil-plant relationship
        1.1.3 Mechanism of uptake heavy metal into plant
    1.2 Phytoremediation
        1.2.1 Detoxification mechanisms in plants
        1.2.2 Using plant for phytoremediation
    1.3 Introduction about sorghum
        1.3.1 Sweet sorghum and application value
        1.3.2 Respones of sweet sorghum to heavy metal
    1.4 Principles of transcriptome analysis and gene expression quantification
        1.4.1 m RNA seq- transcriptome
        1.4.2 Identification of gene expression of plant responses to adverseenvironmental condition
CHAPTER II PHOTOSYNTHESIS AND GROWTH RESPONSE OF SWEET SORGHUM PLANTSTO DIFFERENT LEVELS OF HEAVY METAL CADMIUM
    2.1 Introduction
    2.2 Material and methods
        2.2.1 Plant materials and growth conditions
        2.2.2 Growth characteristic analysis
        2.2.3 Photosynthesis analysis
        2.2.4 Data analysis
    2.3 Results
        2.3.1 Growth characteristic
        2.3.2 Effect of Cd2+ on plant photosynthesis parameters
    2.4 Discussion
    2.5 Conclusions
CHAPTER III CADMIUM ACCUMULATION IN PLANT AND EFFECT ON NUTRIENTELEMENTS
    3.1 Introduction
    3.2 Material and methods
        3.2.1 Plant materials and growth conditions
        3.2.2 Sampling methods
        3.2.3 Measurement of metal concentration
        3.2.4 Data analysis
    3.3 Result
        3.3.1 Cd concentration in organs of sweet sorghum at the seedling stage .. 50
        3.3.2 Cd concentration in organs of sweet sorghum at the hard dough stage
        3.3.3 Effects of cadmium on uptake and translocation of nutrient elements
    3.4 Discussion
        3.4.1 Distribution of Cd in sweet sorghum
        3.4.2 Effect of Cd on nutrient elements transport in plant
    3.5 Conclusions
CHAPTER IV CADMIUM INDUCED MODIFICATION OF SUGAR ACCUMULATION ANDTRANSLOCATION ALONG SWEET SORGHUM STEM
    4.1 Introduction
    4.2 Material and methods
        4.2.1 Plant materials and growth conditions
        4.2.2 Measure sugar content
        4.2.3 Data analysis
    4.3 Results
        4.3.1 Cd effect on total soluble solid in stem of sweet sorghum
        4.3.2 The relationship between Cd concentration and and Brix
        4.3.3 Cd effect on Soluble Sugar in stem of sweet sorghum
    4.4 Discussion
        4.4.1 Cd effect on Brix
        4.4.2 Cd effect on soluble sugar
    4.5 Conclusions
CHAPTER V TRANSCRIPTOME ANALYSIS OF SWEET SORGHUM IN RESPONSE TOCADMIUM STRESSES
    5.1 Introduction
    5.2 Material and methods
        5.2.1 Plant Growth Conditions
        5.2.2 Harvesting plant material
        5.2.3 Sequencing Protocol
    5.3 Results
        5.3.1 Isolation of Total RNA results
        5.3.2 Illumina Sequencing and the Assembly of Sequence Reads
        5.3.3 Differential Expression Analysis
        5.3.4 Gene Ontology (GO) Functional Enrichment Analysis for the DEGs
        5.3.5 KEGG pathway enrichment analysis of differential gene
        5.3.6 Functional analyses of differentially expressed genes
    5.4 Conclusions
CONCLUSSION AND OUTLOOK
REFERENCES
Acknowledgements
RESEARCH ARTICLES PUBLISHED



本文編號(hào)：3812438