發(fā)布時(shí)間：2019-01-14 09:54

【摘要】：為了深入探究DON(溶解性有機(jī)氮)在湖泊水生生態(tài)系統(tǒng)中的重要營(yíng)養(yǎng)作用和生態(tài)效應(yīng),尋找科學(xué)的DON生物有效性評(píng)價(jià)方法,選擇洱海不同湖區(qū)表層沉積物樣品,通過室內(nèi)接種細(xì)菌和銅綠微囊藻進(jìn)行對(duì)比培養(yǎng),運(yùn)用三維熒光-平行醫(yī)療法(3D EEM-PARAFAC)研究菌藻對(duì)比培養(yǎng)條件下湖泊沉積物DON生物有效性及各DON熒光組分生物有效性差異.結(jié)果表明:(1)藻類培養(yǎng)條件下洱海表層沉積物DON生物有效性(8.49%~42.5%)略低于細(xì)菌培養(yǎng)條件下的生物有效性(10.5%~45.3%).(2)藻培養(yǎng)條件下,DON生物利用率與藻細(xì)胞密度增長(zhǎng)量呈顯著正相關(guān),即藻細(xì)胞密度的增長(zhǎng)量可作為反映沉積物DON生物有效性的指標(biāo).(3)菌類培養(yǎng)過程中DON光譜學(xué)特征參數(shù)[如SUVA_(254)(254 nm處紫外吸收光譜系數(shù))、S_R (275~295 nm處吸收光譜系數(shù)與350~400 nm處吸收光譜系數(shù)比值)等]與DON含量相關(guān)性總體較細(xì)菌培養(yǎng)好;培養(yǎng)液中SUVA_(254)有不同程度下降,S_R 指數(shù)均有上升,大分子DON和高芳香性DON可被微生物降解.相比于陸源熒光組分,內(nèi)源熒光組分的生物有效性較差.(4)相比而言,藻類培養(yǎng)方法能更直觀地反映沉積物DON對(duì)藻類生長(zhǎng)的影響,可在一定程度上作為衡量湖泊沉積物DON生物有效性的指標(biāo),即藻類培養(yǎng)方法更適合評(píng)價(jià)洱海沉積物DON生物有效性.
[Abstract]:In order to explore the important nutrition and ecological effects of DON (dissolved organic nitrogen) in lake aquatic ecosystem, a scientific method for evaluating the bioavailability of DON was found, and samples of surface sediments from different lakes of Erhai Lake were selected. By inoculating bacteria and microcystis aeruginosa in laboratory, the bioavailability of DON in lake sediments and the bioavailability of DON fluorescence components were studied by using three-dimensional fluorescence parallel medical method (3D EEM-PARAFAC) under the condition of contrast culture of microcystis aeruginosa and microcystis aeruginosa. The results showed that: (1) the bioavailability of DON in the surface sediments of Erhai Lake (8.4942.5%) was slightly lower than that under bacteria culture (10.55.3%). (_ 2). The bioavailability of DON was positively correlated with the increase of algal cell density. That is to say, the increase of cell density of algae can be used as an index to reflect the bioavailability of DON in sediment. (3) the characteristic parameters of DON spectroscopy in the culture of bacteria [e.g. SUVA_ (254) (UV absorption coefficient at 254 nm), The ratio of absorption spectrum coefficient at 275 ~ 295 nm to absorption coefficient at 350 ~ 400 nm, etc., etc.) the correlation with DON content was generally better than that in bacterial culture. SUVA_ (254) in culture medium decreased to some extent, and SR index increased. Macromolecule DON and high aromatic DON could be degraded by microorganism. The bioavailability of endogenous fluorescent components is lower than that of terrestrial fluorescence components. (4) compared with terrestrial fluorescence components, algae culture method can more directly reflect the effect of sediment DON on algae growth. To some extent, the algae culture method is more suitable for evaluating the bioavailability of DON in lake sediments, that is to say, algae culture method is more suitable for evaluating the bioavailability of DON in lake sediments.
【作者單位】： 北京化工大學(xué);中國(guó)環(huán)境科學(xué)研究院環(huán)境基準(zhǔn)與風(fēng)險(xiǎn)評(píng)估國(guó)家重點(diǎn)實(shí)驗(yàn)室;中國(guó)環(huán)境科學(xué)研究院國(guó)家環(huán)境保護(hù)湖泊污染控制重點(diǎn)實(shí)驗(yàn)室湖泊生態(tài)環(huán)境創(chuàng)新基地;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(U1202235,41503113) 環(huán)境基準(zhǔn)與風(fēng)險(xiǎn)評(píng)估國(guó)家重點(diǎn)實(shí)驗(yàn)室自由探索項(xiàng)目(2014-GOT-042-N-06)
【分類號(hào)】：X524

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