【摘要】：交替沉積自組裝法由于具有簡(jiǎn)單快捷、膜結(jié)構(gòu)可控,不受基底形狀限制等諸多優(yōu)點(diǎn)近年來在藥物緩釋涂層領(lǐng)域受到了廣泛的關(guān)注�；诮惶娉练e自組裝法人們已經(jīng)可以將各種不同種類的藥物分別載入藥物緩釋涂層中,但是到目前為止還缺少一種同時(shí)加載不同類型的藥物到一個(gè)多層膜中的有效方法�；谶@個(gè)問題,本文將載藥聚乳酸納米微粒引入到交替沉積膜中,發(fā)展一種制備藥物控釋涂層的新方法。該方法具有可以將各種不同類型的藥物引入到多層膜中,實(shí)現(xiàn)單一藥物控釋涂層對(duì)多種不同類型藥物的負(fù)載等多方面的優(yōu)點(diǎn),將為基于交替沉積技術(shù)的載藥多層膜的制備提供一個(gè)新的途徑。 本文首先利用自乳化溶劑擴(kuò)散方法制備了包覆有模型藥物芘的聚乳酸納米微粒,并運(yùn)用掃描電子顯微鏡、透射電子顯微鏡、動(dòng)態(tài)光散射等對(duì)微球的表面形貌、直徑大小、表面電位等進(jìn)行了表征,然后利用聚乳酸納米微粒和聚陽離子電解質(zhì)聚乙酰亞胺的交替沉積制備得到了載藥多層膜。用紫外可見光譜對(duì)膜的組裝過程進(jìn)行了跟蹤,用傅里葉變換紅外光譜分析膜的組成,用掃描電子顯微鏡對(duì)膜的形貌進(jìn)行了觀察,對(duì)加載到多層薄膜中的芘的含量也用熒光光譜進(jìn)行了測(cè)定。最后進(jìn)一步研究了交替沉積薄膜中芘的體外釋放情況。實(shí)驗(yàn)結(jié)果表明,該交替沉積膜具有持久地釋放芘的預(yù)期能力。由于聚乳酸納米微粒具有良好的生物相容性和生物可降解性,因此該膜在生物材料、組織工程以及基因工程等領(lǐng)域都具有廣泛的應(yīng)用前景。
[Abstract]:The alternating deposition self-assembly method has attracted wide attention in the field of drug sustained-release coatings in recent years because of its advantages such as simple and fast, controllable membrane structure and not limited by substrate shape. Based on the alternative deposition self-assembly method, different kinds of drugs can be loaded into the drug sustained-release coating, but so far there is still a lack of an effective method to load different kinds of drugs into a multilayer film at the same time. Based on this problem, a new method of preparing drug controlled release coatings was developed by introducing drug loaded polylactic acid nanoparticles into alternate deposition films. The method has the advantages of introducing various kinds of drugs into the multilayer film and realizing the loading of a single drug controlled release coating on a variety of different types of drugs. It will provide a new way for the preparation of multilayer film based on alternating deposition technology. In this paper, polylactic acid nanoparticles coated with model drug pyrene were prepared by self-emulsifying solvent diffusion method, and the surface morphology and diameter of the microspheres were investigated by scanning electron microscope, transmission electron microscope and dynamic light scattering. The surface potential was characterized and then the multilayer films were prepared by alternating deposition of polylactic acid nanoparticles and polycationic electrolyte polyimide. The composition of the film was analyzed by Fourier transform infrared spectroscopy (FTIR) and the morphology of the film was observed by scanning electron microscope (SEM). The content of pyrene in multilayer films was also determined by fluorescence spectra. Finally, the in vitro release of pyrene from alternating deposition films was studied. The experimental results show that the film has the expected ability to release pyrene permanently. Due to its good biocompatibility and biodegradability, the membrane has wide application prospects in biomaterials, tissue engineering and genetic engineering.
【學(xué)位授予單位】：東北大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：TB383.2

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