發(fā)布時(shí)間：2018-07-05 12:22

  本文選題：超臨界二氧化碳注入法 + α-生育酚��； 參考：《上海交通大學(xué)》2011年碩士論文

【摘要】：生物活性物質(zhì)常作為功能食品用來提高人體的某些生理機(jī)能,但是這些物質(zhì)通常難溶于水且不穩(wěn)定、易被氧化,生物利用度很低。將活性物與有特殊物理化學(xué)性物的載體制成復(fù)合物不僅可以提高活性物的穩(wěn)定性,還可以增大活性物的生物利用度。傳統(tǒng)的復(fù)合物制備方法會導(dǎo)致有機(jī)溶劑殘留并且有可能破壞活性物的生物活性,限制了產(chǎn)品的應(yīng)用。超臨界二氧化碳適中的臨界溫度和惰性氣體性質(zhì)可以避免熱敏性和易氧化物質(zhì)的破壞;其低粘度,低表面張力和高滲透性以及無需后續(xù)處理,大大節(jié)省了時(shí)間,提高了制備效率。本文選擇α-生育酚、葉黃素、亞麻酸乙酯等作為維生素類、類胡蘿卜素類、抗氧化劑類以及不飽和脂肪酸類的模型物,以β-環(huán)糊精和介孔二氧化硅為載體進(jìn)行復(fù)合物制備研究。本文研究內(nèi)容和結(jié)果如下: 1.以β-環(huán)糊精為載體,α-生育酚為模型物,采用超臨界二氧化碳注入法制備α-生育酚/β-環(huán)糊精固體復(fù)合物�？疾炝俗⑷雺毫�、注入溫度和注入時(shí)間等參數(shù)對α-生育酚負(fù)載量的影響以及復(fù)合物在水相中的分散性。用紫外分光光度法測定了復(fù)合物的負(fù)載量,FTIR、DSC和TGA對復(fù)合物進(jìn)行了表征。實(shí)驗(yàn)結(jié)果表明:隨著注入壓力和溫度的增加,α-生育酚的負(fù)載量先增大后減小,當(dāng)溫度為35℃,壓力為18MPa,時(shí)間為1h時(shí),負(fù)載量最高達(dá)38.7%;制備的復(fù)合物能在水相中均勻分散。 2.以介孔二氧化硅為載體,α-生育酚為模型物,采用超臨界二氧化碳注入法制備了α-生育酚/介孔二氧化硅固體復(fù)合物。研究過程中,成功制備了不同規(guī)格的介孔硅;利用紫外分光光度法分析了注入前后α-生育酚的結(jié)構(gòu);采用照相機(jī)和SEM分析考察了α-生育酚分布和分散情況;考察了過程參數(shù)注入壓力和溫度,不同的二氧化硅對α-生育酚負(fù)載量的影響。實(shí)驗(yàn)結(jié)果表明:隨著注入壓力和溫度的增加,α-生育酚的負(fù)載量先增大后減小,當(dāng)溫度為35℃,壓力為18MPa,介孔二氧化硅為SBA-15③時(shí),負(fù)載量最高達(dá)79.88%。 3.以介孔二氧化硅為載體,葉黃素及亞麻酸乙酯為模型物,制備了活性物復(fù)合物。并對葉黃素復(fù)合物的穩(wěn)定性進(jìn)行了研究,研究結(jié)果表明制備的復(fù)合物中的活性物質(zhì)穩(wěn)定性大大提高。 通過本文研究,初步掌握了超臨界CO2注入法制備活性物復(fù)合物的規(guī)律;制備復(fù)合物時(shí),過程參數(shù)、載體以及活性物對復(fù)合物中活性物負(fù)載量的影響;建立了制備活性物復(fù)合物新工藝。
[Abstract]:Bioactive substances are often used as functional foods to improve some physiological functions of human body, but these substances are usually insoluble in water, unstable, easy to be oxidized and low bioavailability. The stability of the active compound and the bioavailability of the active compound can be increased by making the complex with the carrier with special physical and chemical properties. Traditional complex preparation methods lead to residual organic solvents and may destroy the biological activity of the active compounds, which limits the application of the products. The moderate critical temperature of supercritical carbon dioxide and the properties of inert gases can avoid the destruction of thermosensitivity and oxidizing substances; their low viscosity, low surface tension and high permeability, as well as the need for follow-up treatment, greatly save time, The preparation efficiency is improved. In this paper, 偽 -tocopherol, lutein, ethyl linolenic acid were selected as model materials of vitamins, carotenoids, antioxidants and unsaturated fatty acids. The complexes were prepared with 尾 -cyclodextrin and mesoporous silica as carriers. The research contents and results are as follows: 1. Using 尾 -cyclodextrin as carrier and 偽 -tocopherol as model material, 偽 -tocopherol / 尾 -cyclodextrin solid complex was prepared by supercritical carbon dioxide injection. The effects of injection pressure, injection temperature and injection time on the loading of 偽 -tocopherol and the dispersion of 偽 -tocopherol in aqueous phase were investigated. The complex was characterized by FTIR DSC and TGA. The experimental results show that the loading amount of 偽 -tocopherol increases first and then decreases with the increase of injection pressure and temperature. When the temperature is 35 鈩，

本文編號：2100191