本文選題：π鍵二苯甲酮異構(gòu)體 + 環(huán)大葉藤黃醇和異大葉藤黃醇　； 參考：《天然產(chǎn)物研究與開發(fā)》2017年07期

【摘要】：本研究報(bào)道了一種制備人面果中π鍵二苯甲酮異構(gòu)體環(huán)大葉藤黃醇和異大葉藤黃醇的液相色譜研究方法。實(shí)驗(yàn)中通過色譜柱以及流動相考察,在分析色譜柱上實(shí)現(xiàn)了液相方法的建立、模擬及篩選試驗(yàn),并在半制備色譜柱上實(shí)現(xiàn)了放大試驗(yàn)和進(jìn)樣量考察。優(yōu)化后的色譜條件可實(shí)現(xiàn)異構(gòu)體達(dá)到2.5的分離度,并在濃度0.2μg/μL時(shí)的進(jìn)樣量達(dá)到500μL,單體化合物環(huán)大葉藤黃醇和異大葉藤黃醇可通過液相色譜、核磁共振DEPT譜及質(zhì)譜予以確認(rèn)。該研究表明,常規(guī)C_(18)色譜柱能夠?qū)Ζ墟I異構(gòu)體進(jìn)行拆分,將加快更多具有生物活性的二苯甲酮單體化合物的發(fā)現(xiàn)。
[Abstract]:In this paper, a liquid chromatographic method for preparation of 蟺 -bonded benzophenone isomers from human noodle fruit, cymbiferol and isobalpinol, is reported. In the experiment, the liquid method was established, simulated and screened on the analytical column by means of chromatographic column and mobile phase inspection, and the amplification test and sample quantity investigation were realized on the semi-prepared chromatographic column. The optimized chromatographic conditions can achieve the separation degree of 2.5 isomers, and the injection amount of the isomers reaches 500 渭 L at the concentration of 0.2 渭 g / 渭 L, and the monomers can be confirmed by liquid chromatography, nuclear magnetic resonance spectroscopy and mass spectrometry. The results show that the conventional C _ (18) column can separate 蟺 -bond isomers, which will accelerate the discovery of more bioactive benzophenone monomers.
【作者單位】： 中南民族大學(xué)天然產(chǎn)物化學(xué)研究室;中南民族大學(xué)藥學(xué)院;中南民族大學(xué)化學(xué)與材料科學(xué)學(xué)院;
【基金】：國家自然科學(xué)基金(31000157) 湖北省高校優(yōu)秀中青年科技創(chuàng)新團(tuán)隊(duì)計(jì)劃(T201220)
【分類號】：O657.72;R284.1
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本文編號：2059202