本文選題：綠豆蛋白 + 酶解��； 參考：《湖北工業(yè)大學(xué)》2011年碩士論文

【摘要】：本文以綠豆粉絲廢水為原料,采用酶解技術(shù)制備具有降血壓功能的ACE抑制肽,利用膜技術(shù)對(duì)ACE抑制肽進(jìn)行初步分離純化;利用大孔吸附樹(shù)脂對(duì)超濾透過(guò)液進(jìn)行靜態(tài)吸附和解吸,動(dòng)態(tài)吸附和解吸,進(jìn)一步純化ACE抑制肽。 1、選取中性蛋白酶、堿性蛋白酶、胃蛋白酶、木瓜蛋白酶對(duì)綠豆蛋白進(jìn)行酶解實(shí)驗(yàn),以水解度和ACE抑制率為指標(biāo),結(jié)果表明堿性蛋白酶水解度和ACE抑制率均高于其它蛋白酶。經(jīng)微波處理后的綠豆蛋白的水解度較經(jīng)熱處理后的綠豆蛋白的水解度高,實(shí)驗(yàn)確定微波預(yù)處理的合適功率為500W,時(shí)間為30s。 通過(guò)單因素實(shí)驗(yàn)和響應(yīng)曲面法確定堿性蛋白酶最佳水解條件為:底物濃度2%,pH7.5,水解時(shí)間120min,加酶量3%,水解溫度55℃;堿性蛋白酶最佳水解條件下,ACE抑制率為84.83%,分子量分布比較寬,其水解體系中還存在著較多較大分子量的多肽。 2、以膜通量和ACE抑制率為考察指標(biāo),比較了四種不同截留分子量的超濾膜對(duì)膜通量和ACE抑制率的影響,結(jié)果表明截留分子量3000的超濾膜膜通量相對(duì)較大,透過(guò)液的ACE抑制率較高達(dá)到87.32%。 通過(guò)單因素實(shí)驗(yàn)和正交實(shí)驗(yàn)確定超濾膜的最佳工藝參數(shù):料液溫度35℃,超濾時(shí)間10min,超濾壓力0.08MPa,pH8,在此條件下,平均膜通量達(dá)到32.71%。利用高效液相色譜分析超濾透過(guò)液分子量分布,發(fā)現(xiàn)分子量大部分在700以下,達(dá)到了初步分離純化的目的。 3、以樹(shù)脂對(duì)多肽的吸附性能和吸附率為指標(biāo)篩選最優(yōu)樹(shù)脂,實(shí)驗(yàn)表明DA201-C樹(shù)脂的吸附性能較好,對(duì)綠豆多肽的吸附率達(dá)到70.45%。采用DA201-C大孔吸附樹(shù)脂能有效脫去超濾透過(guò)液中的鹽分,使用濃度為30%,50%,70%,90%的乙醇對(duì)超濾透過(guò)液進(jìn)行洗脫,實(shí)驗(yàn)結(jié)果表明經(jīng)濃度為70%乙醇洗脫的組分ACE抑制活性最高,ACE抑制率達(dá)到91.23%,相比超濾透過(guò)液提高了5.78%。對(duì)ACE抑制肽達(dá)到較好的純化目的,說(shuō)明利用大孔吸附樹(shù)脂處理超濾透過(guò)液對(duì)降血壓肽段起到了一定的富集純化作用。
[Abstract]:ACE inhibitory peptides with hypotensive function were prepared by enzymatic hydrolysis from mung bean vermicelli wastewater. ACE inhibitory peptides were preliminarily separated and purified by membrane technology, and the macroporous adsorption resin was used for static adsorption and desorption of ultrafiltration permeates. After dynamic adsorption and desorption, ACE inhibitory peptide was further purified. 1. Neutral protease, alkaline protease, pepsin and papain were selected for enzymatic hydrolysis of mung bean protein. The degree of hydrolysis and the inhibition rate of ACE were used as indexes. The results showed that the degree of hydrolysis of alkaline protease and the inhibition rate of ACE were higher than those of other proteases. The hydrolysis degree of mung bean protein after microwave treatment was higher than that of heat treated mung bean protein. The optimum power of microwave pretreatment was 500W and the time was 30s. The optimum hydrolysis conditions of alkaline protease were determined by single factor experiment and response surface method. The optimum hydrolysis conditions were as follows: substrate concentration 2: pH 7.5, hydrolysis time 120 min, enzyme addition 3 min, hydrolysis temperature 55 鈩，

本文編號(hào)：2053084