本文選題：超臨界二氧化碳 + 超臨界抗溶劑法��； 參考：《上海交通大學(xué)》2010年碩士論文

【摘要】： 玉米蛋白作為一種天然的生物大分子,具有優(yōu)秀的生物相容性和生物可降解的特性,而且食物蛋白通常被認(rèn)為是安全的營養(yǎng)物,其本身也有營養(yǎng)價值。玉米蛋白已廣泛用于緩釋控釋藥物的載體材料,其中玉米蛋白微球較為常見。其作為載體材料包埋的藥物均具有良好的緩釋性能、且表現(xiàn)出抗一定的抗胃蛋白酶分解的特性,是一種不錯的腸溶性包衣材料。 近年來,超臨界流體抗溶劑技術(shù)已成功應(yīng)用于制備微納米顆粒,因具備溶劑殘留低、產(chǎn)品粒徑小、粒度分布可控、反應(yīng)條件溫和、工藝簡單等優(yōu)勢,該技術(shù)在藥物載體領(lǐng)域中的應(yīng)用受到廣泛的關(guān)注。 本文主要采用超臨界流體提高溶液分散法(SEDS)進(jìn)行制備玉米蛋白基藥物載體,并以葉黃素為模型藥物,制備葉黃素-玉米蛋白納米膠囊。主要研究結(jié)果如下: 玉米蛋白空白微球的制備和表征。采用SEDS法從玉米蛋白的丙酮-二甲亞砜混合溶液中制得了玉米蛋白納米顆粒,考察了溫度、壓力、溶液濃度、二氧化碳流速和溶液流速對玉米蛋白空白微球形貌和粒徑的影響。用掃描電鏡、納米粒度分析、熱重分析、差熱分析等手段對結(jié)果進(jìn)行表征。實驗結(jié)果表明,SEDS法可用于制備球形度較好,粒徑分布較窄的玉米蛋白微球,所得玉米蛋白微球平均粒徑在160-400nm之間。溫度、壓力、溶液濃度都會影響微粒的粒徑,選取不同的工藝組合可制備不同粒徑及分布要求的微粒。熱重分析和差熱分析的結(jié)果顯示制得的玉米蛋白空白微球較原料穩(wěn)定性有所提高。 葉黃素-玉米蛋白納米膠囊的制備和表征。在玉米蛋白空白微球制備的基礎(chǔ)上,選用葉黃素作為模型藥物,采用SEDS法制備葉黃素-玉米蛋白納米膠囊�？疾炝藴囟取毫�、溶液濃度、芯材比和溶液流速對葉黃素納米膠囊形貌、粒徑、載藥量和包埋率的影響。用掃描電鏡、納米粒度分析、紫外-可見光譜分析、差熱分析、X射線衍射分析等手段對結(jié)果進(jìn)行表征。實驗結(jié)果表明,SEDS法可制備出較高載藥量和包埋率的葉黃素-玉米蛋白納米膠囊,溫度、壓力、芯材比、溶液流速都會影響納米膠囊的粒徑、包埋率和載藥量。在溫度為45℃、壓力為10MPa、玉米蛋白溶液濃度為1.5%、芯材比為1:18、溶液流速為1mL/min的最佳條件下制備出的葉黃素納米膠囊載藥量有5.87%,包埋率為60.38%,此時納米膠囊的平均粒徑為205nm。差熱分析和X射線衍射分析的結(jié)果表明,納米膠囊中葉黃素以晶體的形式存在,且葉黃素晶體是被包埋在玉米蛋白中形成葉黃素-玉米蛋白納米膠囊。 最后對全文作了總結(jié),對文章的創(chuàng)新點和不足做了概括并提出后續(xù)發(fā)展的建議。
[Abstract]:As a kind of natural biological macromolecule, corn protein has excellent biocompatibility and biodegradable properties, and food protein is generally considered as a safe nutrient, and it also has nutritional value. Corn protein has been widely used as a carrier material for sustained release and controlled release drugs, among which corn protein microspheres are more common. The drugs which are encapsulated as carrier materials have good slow-release properties and exhibit anti-pepsin decomposition characteristics. They are a good enteric-soluble coating material. In recent years, supercritical fluid antisolvent technology has been successfully applied to the preparation of microparticles, due to the advantages of low solvent residue, small product size, controllable particle size distribution, mild reaction conditions and simple process. The application of this technology in the field of drug carrier has received extensive attention. In this paper, zeaxanthin based drug carrier was prepared by supercritical fluid enhanced solution dispersion method (SEDSs), and lutein was used as model drug to prepare xanthophyll-corn protein nanocapsule. The main findings are as follows: Preparation and characterization of Corn protein Blank Microspheres. Corn protein nanoparticles were prepared by SEDS from a mixture of acetone and dimethyl sulfoxide of corn protein. The effects of temperature, pressure, concentration of solution, flow rate of carbon dioxide and flow rate of solution on the morphology and particle size of corn protein blank microspheres were investigated. The results were characterized by SEM, nano-particle size analysis, thermogravimetric analysis and differential thermal analysis. The results showed that the method could be used to prepare corn protein microspheres with good spherical size and narrow particle size distribution, and the average diameter of the obtained corn protein microspheres was between 160-400nm. The particle size can be affected by temperature, pressure and solution concentration. The results of thermogravimetric analysis and differential thermal analysis showed that the stability of corn protein blank microspheres was higher than that of raw material. Preparation and characterization of lutein-corn protein nanocapsules. Based on the preparation of corn protein blank microspheres, lutein was selected as model drug and xanthophyll-corn protein nanocapsules were prepared by SEDS method. The effects of temperature, pressure, concentration of solution, ratio of core to material and flow rate of solution on morphology, particle size, drug loading and entrapment rate of lutein nanocapsule were investigated. The results were characterized by scanning electron microscopy (SEM), nano-particle size analysis, UV-Vis spectrum analysis, differential thermal analysis (DTA) and X-ray diffraction (DTA). The results showed that high loading and entrapment rate of xanthophyll-corn protein nanocapsule could be obtained by seds method. Temperature, pressure, ratio of core to material, flow rate of solution could all affect the particle size, entrapment rate and drug loading rate of nanocapsule. When the temperature was 45 鈩，

本文編號：1895999