本文選題：大豆異黃酮　切入點(diǎn)：純化　出處：《食品工業(yè)科技》2016年01期

【摘要】：用有機(jī)溶劑萃取和樹脂吸附純化醇酶法制大豆油廢液中的異黃酮,用高效液相色譜檢測純化的異黃酮的純度。以人乳腺癌細(xì)胞系MCF-7為模型,檢測純化的大豆異黃酮對MCF-7增殖的影響。結(jié)果表明,萃取法粗提的最佳萃取溶劑為乙酸乙酯與正丁醇(1∶1)的混合液,異黃酮提取率為70.47%,純度為26.45%;樹脂吸附純化的最佳條件為:樹脂型號(hào),LSA-8;上樣濃度,0.8 mg/m L;上樣流速,0.5 BV/h;洗脫液,75%的乙醇;洗脫流速,6 BV/h;洗脫體積,1.5倍上樣體積,異黃酮提取率為83.71%,純度為72.09%。抗腫瘤實(shí)驗(yàn)表明,大豆異黃酮濃度達(dá)到100 mg/L時(shí),能顯著(p0.01)抑制MCF-7細(xì)胞的增殖,其作用有一定的劑量依賴性。
[Abstract]:Isoflavones from soybean oil waste liquor were purified by solvent extraction and resin adsorption. The purity of isoflavones was determined by HPLC.The effects of purified soybean isoflavones on the proliferation of human breast cancer cell line MCF-7 were studied.The results showed that the optimum extraction solvent was the mixture of ethyl acetate and n-butanol 1: 1).寮傞粍閰彁鍙栫巼涓，

本文編號(hào)：1703955