【摘要】：目的：應(yīng)用腦震蕩(cerebral concussion，CC)大鼠模型，觀察不同損傷程度腦震蕩后不同時間大鼠認(rèn)知障礙變化及酪氨酸羥化酶變化規(guī)律，探討腦震蕩鼠認(rèn)知、記憶障礙的特點(diǎn)及與酪氨酸羥化酶變化的關(guān)系，，以了解腦震蕩鼠認(rèn)知記憶行為障礙的神經(jīng)生物學(xué)基礎(chǔ)，為腦震蕩后綜合癥的臨床診斷、治療提供理論依據(jù)。 方法：應(yīng)用自制金屬單擺式閉合性腦損傷機(jī)械打擊裝置復(fù)制單純性腦震蕩(pure cerebral concussion，PCC)和復(fù)雜性腦震蕩(complicated cerebral concussion，CCC)大鼠模型；均隨機(jī)分為傷后1、2、4、8、16、24天組(n=10)和一個正常對照組(n=10)。應(yīng)用Morris水迷宮(Morris Water Maze，MWM)分別對傷后8天組(1～7天)、16天組(8～14天)、24天組(15～21天)PCC和CCC大鼠的學(xué)習(xí)記憶能力進(jìn)行檢測；應(yīng)用免疫印記法(western blot，WB)于傷后1、2、4、8、16、24天檢測大鼠傷后基底節(jié)、中隔區(qū)的酪氨酸羥化酶(tyrosine hydroxylase，TH)變化規(guī)律。 結(jié)果：在MWM中，PCC后大鼠在傷后3天找到平臺的時間顯著延長(P＜0.05)，但PCC后8～14d、15～21d差異無顯著性。CCC大鼠在傷后2周找到平臺的時間顯著延長，傷后6天及傷后第10～11天差異具有顯著性(P＜0.05)，15～21d無顯著性差異；PCC后不同時間點(diǎn)基底節(jié)TH在傷后表達(dá)總體呈上升趨勢，傷后第一天開始上升，2天組、4天組稍下降但仍高于對照組，8天組、16天組和24天組持續(xù)高表達(dá)，與對照組比較差異均具有顯著性(p＜0.05)。中隔區(qū)TH在傷后表達(dá)呈上升趨勢，與對照組比較差異均具有顯著性(p
[Abstract]:Objective: to observe the changes of cognitive impairment and tyrosine hydroxylase in rats with concussion (cerebral concussion,CC) at different time after concussion, and to explore the cognition of concussion rats. In order to understand the neurobiological basis of cognitive memory behavior disorder in concussion mice and to provide theoretical basis for clinical diagnosis and treatment of post-concussion syndrome, the characteristics of memory impairment and its relationship with the changes of tyrosine hydroxylase were studied in order to understand the neurobiological basis of cognitive memory behavior disorder in concussion mice. Methods: the rat models of simple concussion (pure cerebral concussion,PCC) and complex concussion (complicated cerebral concussion,CCC) were established by using self-made metal pendulum closed brain injury mechanical strike device. They were randomly divided into 1, 2, 4, 8, 16, 24-day group (n 鈮

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