【摘要】：目的：觀察三重腦震蕩鼠軸索、髓鞘變化規(guī)律及淀粉樣前體蛋白(APP)、軸突生長(zhǎng)抑制因子(Nogo)表達(dá)變化規(guī)律，為運(yùn)動(dòng)相關(guān)性腦震蕩(SRCC)的防治提供一定的理論依據(jù)。方法：用金屬單擺打擊裝置復(fù)制三重腦震蕩大鼠模型，隨機(jī)分為傷后1、2、4、8、16、24天組共六個(gè)損傷組(每組n=8)，同時(shí)設(shè)正常對(duì)照組(n=8)。采用Bielschowsky銀染顯示軸索及Weil氏染色顯示髓鞘，用抗-APP和抗-Nogo多克隆抗體行免疫組化(S-P)法染色。光鏡下對(duì)腦干軸索APP及Nogo陽(yáng)性反應(yīng)的強(qiáng)弱進(jìn)行定性分析；用圖像分析系統(tǒng)分別對(duì)銀染腦干軸索直徑(um)、髓鞘變性程度及海馬、頂葉皮質(zhì)、腦干APP及Nogo陽(yáng)性反應(yīng)進(jìn)行定量分析。統(tǒng)計(jì)學(xué)處理用單因素方差分析、q檢驗(yàn)進(jìn)行組間多個(gè)樣本比較及兩兩比較。結(jié)果：1．三重腦震蕩損傷后各組均不同程度出現(xiàn)軸索改變，包括軸索腫脹、變形、排列紊亂、斷裂甚至軸索收縮球等。對(duì)銀染軸索直徑平均值(urn)進(jìn)行定量分析，，結(jié)果為傷后各組軸索直徑均大于對(duì)照組，以2、4天組最明顯(P＜0.05)。2．髓鞘染色顯示傷后各組均不同程度出現(xiàn)髓鞘腫脹、淡染、變性。對(duì)其著色程度進(jìn)行灰度值定量分析，傷后除24天組外，其余各組與對(duì)照組相比均有顯著性差異(P＜0.05)，以2、4天組最顯著。3．APP在神經(jīng)元及軸索中均見(jiàn)有陽(yáng)性表達(dá)，損傷后先增強(qiáng)，之后降低，再上調(diào)至接近正常。其中在腦干軸索APP表現(xiàn)為1天組增強(qiáng)，隨后下降，至4天組達(dá)低峰，之后逐漸上升恢復(fù)正常；在腦干網(wǎng)狀結(jié)構(gòu)神經(jīng)元的1、4、16天組與對(duì)照組相比有顯著性差異(P＜0.05)；在海馬CA3區(qū)神經(jīng)元的1、16天組與對(duì)照組相比有顯著性差異(P＜0.05)；在頂葉皮質(zhì)神經(jīng)元的1、2天組與對(duì)照組相比有顯著性差異(P＜0.05)。4．Nogo在神經(jīng)元及軸索纖維中均有陽(yáng)性表達(dá)，傷后表現(xiàn)為先增強(qiáng)然后逐漸恢復(fù)，不同部位時(shí)間變化規(guī)律不同。其中在腦干軸索以4天組最強(qiáng)；在腦干網(wǎng)狀結(jié)構(gòu)神經(jīng)元的1、2、4、8天組與對(duì)照組相比有顯著性差異(P＜0.05)；在海馬CA3區(qū)神經(jīng)元的2、4天組與對(duì)照組相比有顯著性差異(P＜0.05)；在大腦頂葉皮質(zhì)神經(jīng)元的4天組與對(duì)照組相比有顯著性差異(P＜0.05)。結(jié)論：1．三重腦震蕩
[Abstract]:Aim: to observe the changes of axons, myelin sheath and (Nogo) expression of amyloid precursor protein (APP), axon growth inhibitor in rats with triple concussion, so as to provide a theoretical basis for the prevention and treatment of exercise-related concussion (SRCC). Methods: the rat model of tricuspid concussion was made by using a metal pendulum striking device. The rats were randomly divided into six injury groups (n = 8) and normal control group (n = 8) on day 1, 2, 4, 8, 16, 24 days after injury. The rats were divided into two groups: the control group (n = 8) and the control group (n = 8). Bielschowsky silver staining and Weil's staining were used to show myelin sheath, and anti-APP and anti-Nogo polyclonal antibodies were used for immunohistochemical staining. The positive reaction of APP and Nogo in brainstem axons was qualitatively analyzed under light microscope. The degree of myelin degeneration and the positive reaction of APP and Nogo in hippocampus, parietal cortex, brainstem and brain stem of silver stained brainstem axons (um),) were quantitatively analyzed by image analysis system. One-way ANOVA and Q-test were used to compare multiple samples and pairwise. Results: 1. Axonal changes, including axonal swelling, deformation, disorder of arrangement, breakage and even axonal contractile ball, were found in all groups after triple concussion injury. The average diameter of silver stained axons (urn) was quantitatively analyzed. The results showed that the axonal diameter of each group was larger than that of the control group after injury, and the most obvious was at the 2nd and 4th day after injury (P < 0.05). 2. Myelin sheath staining showed that myelin sheath swelling appeared in different degrees in all groups after injury. Light dyeing, denaturation. The grayscale analysis showed that there was significant difference between the other groups except the 24-day group after injury (P < 0.05), the most significant expression of app was found in neurons and axons in the 2-and 4-day groups, and the positive expression of app was found in the neurons and axons in the 4-day group, except for the 24-day group (P < 0.05). The damage was enhanced after injury, then decreased, and then up-regulated to close to normal. The APP of brainstem axons was enhanced in 1 day group, then decreased, reached a low peak in 4 days group, then returned to normal gradually, and there was significant difference in 1,4,16 days group compared with the control group (P < 0.05). There was significant difference between the 16-day group and the control group (P < 0.05). The expression of 4.Nogo in neurons and axonal fibers of parietal cortex was significantly higher than that of control group (P < 0.05) at 1 day and 2 days (P < 0.05), and the expression of VEGF in neurons and axonal fibers was enhanced at first and then recovered gradually after injury. The time variation of different parts is different. The brain stem axons were the strongest in the 4-day group, and there was significant difference between the 1, 2, 4 and 8-day groups compared with the control group (P < 0.05). There was a significant difference between the 4-day group and the control group (P < 0.05), and there was a significant difference between the 4-day group and the control group in the 4-day group of hippocampal CA3 neurons (P < 0.05). Triple concussion
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：D919

【參考文獻(xiàn)】

相關(guān)期刊論文 前5條

1 于曉軍,肖飛,吳家櫦,吳梅筠;大鼠閉合性腦損傷酯化銀和白蛋白組織化學(xué)研究[J];法醫(yī)學(xué)雜志;2001年02期

2 朱金龍,朱少華,任亮,劉良,周亦武,陳虎,鄧偉年;大鼠彌散性軸索損傷后β-APP的表達(dá)[J];法醫(yī)學(xué)雜志;2005年03期

3 李宗平;顱腦損傷后β-APP及其產(chǎn)物在腦組織中的變化及臨床意義[J];國(guó)外醫(yī)學(xué)(神經(jīng)病學(xué)神經(jīng)外科學(xué)分冊(cè));2003年01期

4 于建云,李樹華,韓曉華,許冰瑩,林海英,趙旭東;腦震蕩鼠細(xì)胞組織病理學(xué)研究[J];昆明醫(yī)學(xué)院學(xué)報(bào);2003年02期

5 劉雁,石尚金,董為偉;腦梗死后自發(fā)性高血壓大鼠腦內(nèi)Nogo-A mRNA的表達(dá)[J];中國(guó)臨床康復(fù);2003年05期

本文編號(hào)：2450507