發(fā)布時間：2019-02-13 01:06

【摘要】： 近年來,受世界毒潮泛濫的影響,我國禁毒斗爭形式依然十分嚴重。在海洛因類毒品沒有得到有效遏制的情況下,苯丙胺類毒品已經(jīng)取代海洛因類毒品成為我國的主要毒品。因此,迫切需要建立一種準確、快速檢測生物樣品中苯丙胺類和海洛因類毒品的方法。本文分別利用微波萃取法和液液萃取法對血液和尿液中的苯丙胺類和海洛因類毒品進行了研究。 建立了人體血液中甲基苯丙胺(MA),3,4-亞甲二氧基苯丙胺(MDA),3,4-亞甲二氧基甲基苯丙胺(MDMA)的微波萃取-氣相色譜(GC)和氣相色譜/質(zhì)譜(GC/MS)測定方法。分別考察了萃取溶劑種類、用量、pH以及萃取溫度、時間等因素對萃取率的影響。實驗結(jié)果表明,血液中MA,MDA,MDMA的最佳提取條件為:調(diào)節(jié)血樣pH為13,以乙酸乙酯為萃取溶劑,于30℃下微波提取10 min。該方法未對藥物進行衍生化,是一種快速,準確,靈敏度高的測定血液樣品中MA,MDA,MDMA的方法。 建立了人體尿液中MA,MDA,MDMA的微波萃取-氣相色譜(GC)和氣相色譜/質(zhì)譜(GC/MS)測定方法。分別考察了萃取溶劑種類、用量、pH以及萃取溫度、時間等因素對萃取率的影響。實驗結(jié)果表明,尿液中MA,MDA,MDMA的最佳提取條件為:調(diào)節(jié)尿樣pH為12,以環(huán)己烷為萃取溶劑,于40℃下微波提取10 min。該方法未對藥物進行衍生化,是一種快速,準確,靈敏度高的測定尿液樣品中MA,MDA,MDMA的方法。 建立了人體血液中的可待因、嗎啡、3-單乙酰嗎啡(O3)的測定方法,分別考察了不同萃取溶劑、萃取時間、溶液的pH和用量對萃取結(jié)果的影響,以及不同衍生化試劑和用量對檢出限的影響。該方法操作簡單,大大提高了樣品前處理的效率。
[Abstract]:In recent years, influenced by the flood of drugs in the world, the fight against drugs in our country is still very serious. Without the effective control of heroin drugs, amphetamines have replaced heroin drugs as the main drugs in China. Therefore, there is an urgent need to establish an accurate and rapid method for the detection of amphetamines and heroin in biological samples. The amphetamines and heroin drugs in blood and urine were studied by microwave extraction and liquid extraction respectively. A method for the determination of methamphetamine (MA), _ 3N) _ 4-methylenedioxyamphetamine (MDA), _ (3) -4-methylenedioxymethamphetamine (MDMA) in human blood by microwave extraction-gas chromatography (GC) and gas chromatography / mass spectrometry (GC/MS) was developed. The effects of solvent type, amount, pH, extraction temperature and time on the extraction rate were investigated. The results showed that the optimal extraction conditions of MA,MDA,MDMA in blood were as follows: adjusting the pH of blood sample to 13, using ethyl acetate as extraction solvent and microwave extracting 10 min. at 30 鈩，

本文編號：2421001