【摘要】：目的對6個短串聯(lián)重復(fù)序列(Short Tandem Repeats,STR)基因座在成都漢族群體中的遺傳多態(tài)性及其種屬特異性進(jìn)行研究,探討其在法醫(yī)學(xué)中的應(yīng)用價值,并為法醫(yī)DNA分型提供新的候選STR遺傳標(biāo)記。 方法 用Chelex法從110名成都漢族無血緣關(guān)系個體的新鮮血以及14種常見動物組織中提取DNA。從GDB數(shù)據(jù)庫中查出6個四核苷酸重復(fù)序列D4S2366、D4S2367、D6S474、D6S1281、D2S1396和D20S601,按數(shù)據(jù)庫提供的序列合成引物,經(jīng)PCR擴(kuò)增、非變性聚丙烯酰胺凝膠電泳和硝酸銀染色技術(shù)檢測,對110名成都漢族無血緣關(guān)系個體的6個STR基因座進(jìn)行DNA分型,應(yīng)用POWERSTATS軟件對分型結(jié)果進(jìn)行統(tǒng)計分析,獲得各基因座基因頻率及法醫(yī)遺傳多態(tài)性數(shù)據(jù)。應(yīng)用所合成的引物對14種常見動物DNA進(jìn)行PCR擴(kuò)增和電泳檢測,對6個STR基因座的種屬特異性進(jìn)行研究。 結(jié)果 D4S2366、D4S2367、D6S474、D6S1281、D2S1396和D20S601基因座在成都漢族群體中等位基因個數(shù)分別為7、7、6、7、5和7;觀察雜合度分別為0.802、0.708、0.770、0.627、0.542和
[Abstract]:Objective to study the genetic polymorphism and species specificity of six short tandem repeat (Short Tandem Repeats,STR) loci in Chengdu Han population, and to explore their application value in forensic medicine. And to provide a new candidate STR genetic marker for forensic DNA typing. Methods Chelex method was used to extract DNA. from fresh blood and 14 common animal tissues from 110 unrelated individuals of Chengdu Han nationality. Six tetranucleotide repeats D4S2366, D4S2367, D6S474, D6S1281, D2S1396 and D20S601 were identified from GDB database. Primers were synthesized according to the sequences provided by the database, amplified by PCR, detected by non-denatured polyacrylamide gel electrophoresis and silver nitrate staining. Six STR loci of 110 unrelated individuals in Chengdu Han nationality were genotyped by DNA. The results were statistically analyzed by POWERSTATS software, and the genetic polymorphism data of each locus and forensic genetic polymorphism were obtained. The PCR amplification and electrophoretic detection of DNA of 14 common animals were carried out by using the synthesized primers, and the species-specificity of 6 STR loci were studied. Results the number of alleles in D4S2366-D4S2367-D6S474FN D6S1281D2S1396 and D20S601 locus in Chengdu Han population were 70.7080.7270.542 and 0.8020.7080.7080.7270.542, respectively.
【學(xué)位授予單位】：四川大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：D919

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