【摘要】：目的探討大鼠死后不同時(shí)間腎、脾3-磷酸甘油醛脫氫酶(GAPDH)mRNA的表達(dá)對死亡時(shí)間(PMI)推斷的應(yīng)用價(jià)值。方法28只大鼠斷頸處死后置于20℃溫控系統(tǒng)內(nèi),分別于死后0,8,16,24,32,40,48h共7個(gè)時(shí)相點(diǎn)(n=4)解剖,利用熒光RT-PCR技術(shù)檢測大鼠腎、脾GAPDHmRNA相對表達(dá)量的變化。結(jié)果在大鼠死后40h腎、32h脾內(nèi)可檢測出GAPDHmRNA,且其表達(dá)產(chǎn)物均呈規(guī)律性下降趨勢(熒光強(qiáng)度逐漸變暗并最終消失)。結(jié)論檢測大鼠死后不同時(shí)間腎、脾GAPDHmRNA的表達(dá)對PMI推斷具有一定的應(yīng)用價(jià)值。
[Abstract]:Objective to investigate the value of the expression of 3-phosphoglyceraldehyde dehydrogenase (GAPDH) mRNA) in the kidney and spleen of rats at different time after death in predicting the death time (PMI). Methods 28 rats were killed by cervical amputation and placed in the temperature control system at 20 鈩，

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