發(fā)布時間：2018-07-07 12:12

  本文選題：法醫(yī)學(xué) + 魚類　； 參考：《四川大學(xué)》2006年博士論文

【摘要】：目的 法醫(yī)魚類學(xué)是一門正在興起的邊緣科學(xué)，它是在魚類學(xué)、分子生物學(xué)、分析化學(xué)、生物信息學(xué)等基礎(chǔ)上，特別是汲取了法醫(yī)DNA分析的經(jīng)驗發(fā)展起來的，旨在對各類案件涉及到的魚類樣本進行鑒定，為案件的偵查與審判提供科學(xué)證據(jù)。法醫(yī)魚類學(xué)在魚類資源科學(xué)管理和保護方面有重要作用。本課題旨在研究用于法醫(yī)魚類學(xué)種屬鑒別的新技術(shù)。 方法 選擇鯉形目的5種珍稀魚類作為研究對象，，它們是胭脂魚，重口裂腹魚，巖原鯉，華鯪，四川白甲。另外20種魚作為研究的對照樣本。全部樣本采自長江、嘉陵江、雅江以及重慶和成都水產(chǎn)品市場。根據(jù)GenBank中鯉魚的DNA序列資料，利用引物設(shè)計軟件設(shè)計鯉形目控制合成5.8SrRNA和28SrRNA基因間的轉(zhuǎn)錄間隔子2(Internal Transcribed Spacer 2，ITS2)DNA區(qū)域的通用引物，預(yù)計擴增片段包含完整的ITS2及部分編碼5.8SrRNA和28SrRNA基因的序列。用通用引物對樣本進行PCR擴增，PCR產(chǎn)物用聚丙烯酰胺凝膠電泳分離，回收。對每種要鑒別魚類的回收產(chǎn)物進行DNA雙向測序。根據(jù)測序結(jié)果，用系統(tǒng)進化軟件(Mega3.1)進行鯉形目魚類親緣關(guān)系分析。根據(jù)鯉形目5種魚類ITS2序列的不同，設(shè)計分別針對每種魚的種屬特異
[Abstract]:Objective Forensic fish science is an emerging frontier science. It has been developed on the basis of fish science, molecular biology, analytical chemistry, bioinformatics and so on, especially drawing on the experience of forensic DNA analysis. The purpose of this paper is to identify the fish samples involved in various cases and to provide scientific evidence for the investigation and trial of the cases. Forensic fish science plays an important role in the scientific management and protection of fish resources. The purpose of this paper is to study a new technique for the identification of species in forensic fish. Methods five rare fishes of Cyprinidae were selected as research objects. They were cochineal fish, carmine fish, Carpio carpio, mud carp and Sichuan white nail. Another 20 species of fish were used as control samples in the study. All samples were collected from Yangtze River, Jialing River, Yajiang River and aquatic products markets in Chongqing and Chengdu. Based on the DNA sequence of Cyprinus Carpio in GenBank, a general primer was designed to control the synthesis of 5.8s rRNA and 28s rRNA intergenic intertranscriptional spacer 2 (IS2) DNA region by using primer design software. The amplified fragments are expected to contain complete ITS2 and partial sequences encoding 5.8s rRNA and 28s rRNA genes. The PCR products were separated and recovered by polyacrylamide gel electrophoresis. DNA bidirectional sequencing was performed for each recovery product to be identified from fish. Based on the sequencing results, phylogenetic analysis of Cyprinidae was carried out by means of phylogenetic software (Mega 3.1). According to the different ITS2 sequences of 5 species of Cyprinidae, the specific species of each fish were designed.
【學(xué)位授予單位】：四川大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2006
【分類號】：D919.4

【參考文獻】

相關(guān)期刊論文 前10條

1 俞純方,劉大翠,劉中全,謝浩若,王蒙;溈科四種魚LDH同工酶譜的研究[J];四川職業(yè)技術(shù)學(xué)院學(xué)報;2003年01期

2 李思發(fā),朱澤聞,鄒曙明,趙金良,蔡完其;魴屬團頭魴、三角魴及廣東魴種間遺傳關(guān)系及種內(nèi)遺傳差異[J];動物學(xué)報;2002年03期

3 趙凱;青海湖裸鯉與鯉、鯽、草魚的隨機擴增多態(tài)DNA分析[J];淡水漁業(yè);2001年05期

4 戴鳳田,蘇錦祥;溈科八種魚類同工酶和骨骼特征分析及系統(tǒng)演化的探討(鲇形目:溈科)[J];動物分類學(xué)報;1998年04期

5 陳自明,陳毅峰;用RAPD技術(shù)對特化等級裂腹魚類親緣關(guān)系的探討[J];動物學(xué)研究;2000年04期

6 李志忠,吳婷婷,楊弘;奧利亞羅非魚和尼羅羅非魚的遺傳標(biāo)記[J];甘肅農(nóng)業(yè)大學(xué)學(xué)報;2000年01期

7 宋林生,相建海,周嶺華,張首臨,劉瑞玉;六種海產(chǎn)蝦類基因組DNA多態(tài)性的RAPD標(biāo)記研究[J];海洋與湖沼;1999年01期

8 汪亞平,胡煒,吳剛,孫永華,陳尚萍,張甫英,朱作言,馮建新,張西瑞;轉(zhuǎn)“全魚”生長激素基因鯉魚及其F_1遺傳分析[J];科學(xué)通報;2001年03期

9 邱高峰,常林瑞;我國近海中國對蝦種群遺傳差異的RAPD分析[J];上海水產(chǎn)大學(xué)學(xué)報;2001年01期

10 楊子恒;分子進化樹的統(tǒng)計推斷[J];遺傳;1995年S1期

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