本文選題：銀屑病 + 白細(xì)胞介素-8�。� 參考：《四川大學(xué)》2007年碩士論文

【摘要】： 背景：銀屑病(psoriasis，PS)是一種自身免疫系統(tǒng)慢性調(diào)節(jié)障礙導(dǎo)致的炎性皮膚過(guò)度增生性疾病，在人群中的發(fā)病率約0．1-3％。我國(guó)1984年進(jìn)行的全國(guó)銀屑病流行病學(xué)調(diào)查認(rèn)為總的患病率為0．123％，據(jù)此估計(jì)目前我國(guó)銀屑病患者超過(guò)300萬(wàn)。嚴(yán)重的銀屑病不僅使患者生活質(zhì)量下降，而且醫(yī)藥費(fèi)用高昂，美國(guó)每年在該病醫(yī)療花費(fèi)高達(dá)16億美元，盡管如此，目前依然沒(méi)有有效的治療方法。引起銀屑病的病因與發(fā)病機(jī)制目前尚不清楚。近年來(lái)的流行病學(xué)研究高度提示人們，遺傳因素在其病因?qū)W中具有重要地位。 目的：基于我國(guó)銀屑病的遺傳研究現(xiàn)狀和人類基因組數(shù)據(jù)庫(kù)中IL-8和IL-8R相關(guān)資料，通過(guò)比較健康群體與銀屑病群體在IL-8(-251)、IL-8R(Ser276Thr)、IL-8R(Met31Arg)、IL-8R(Arg335Cys)四個(gè)基因座上的基因型和等位基因頻率，研究其與銀屑病的關(guān)系，發(fā)現(xiàn)銀屑病致病基因或者遺傳易感基因，為銀屑病的基因篩查、診斷、治療等提供理論依據(jù)和技術(shù)支持。 方法：(1)根據(jù)基因組數(shù)據(jù)庫(kù)資料設(shè)計(jì)并合成特異性引物，確定酶切基因座。(2)隨機(jī)選取中國(guó)漢族PS患者105例以及體檢健康者105例作對(duì)照，，抽取外周靜脈血，提取DNA。(3)對(duì)所提取的基因組DNA進(jìn)行聚合酶鏈反應(yīng)-限制性片段長(zhǎng)度多態(tài)性(PCR-RFLP)的方法檢測(cè)。(4)基因型和等位基因頻率采用基因直接計(jì)數(shù)法計(jì)算，各組間基因型及等位基因頻率比較采用SPSS13．0軟件的卡方檢驗(yàn)(χ~)進(jìn)行分析。 結(jié)果：IL-8R(Met31Arg)的GT基因型在PS組出現(xiàn)的例數(shù)高于對(duì)照組，經(jīng)校正性χ~2檢驗(yàn)P=0．003，小于0．05，說(shuō)明組間構(gòu)成有明顯差異；G等位基因在PS組出現(xiàn)的頻率高于對(duì)照組，P=0．032，有統(tǒng)計(jì)學(xué)意義。IL-8R(Arg335Cys)的TT基因型頻率高于對(duì)照組，P=0．003，小于0．05，統(tǒng)計(jì)學(xué)有顯著性意義。IL-8(-251)、IL-8R(Ser276Thr)的基因型和等位基因頻率比較無(wú)統(tǒng)計(jì)學(xué)意義。 結(jié)論：(1)IL-8R(Met31Arg)基因座可能與銀屑病易感性正相關(guān)，會(huì)加大銀屑病的遺傳易感性，可作為銀屑病易感性的一個(gè)判斷指標(biāo)。 (2)IL-8R(Arg335Cys)基因座TT基因型可能調(diào)控IL-8R的表達(dá)和功能，從而銀屑病疾病的發(fā)生和病程相關(guān)聯(lián)。 (3)在所研究人群中未發(fā)現(xiàn)IL-8(-251)、IL-8R(Ser276Thr)基因座基因型和等位基因與銀屑病遺傳易感性相關(guān)。
[Abstract]:Background: psoriasis (psoriasis) is an inflammatory skin hyperproliferative disease caused by chronic regulatory disorders of the autoimmune system. The incidence of psoriasis in the population is about 0.1-3. The prevalence rate of psoriasis was estimated to be more than 3 million in China, according to the national epidemiological survey of psoriasis in 1984. Severe psoriasis not only reduces patients' quality of life, but also costs a lot of medicine. The United States spends as much as $1.6 billion a year on the disease, but there are still no effective treatments. The etiology and pathogenesis of psoriasis are unclear. Epidemiological studies in recent years highly suggest that genetic factors play an important role in its etiology. Objective: to compare the genotypes and alleles frequencies of IL-8 and IL-8R on IL-8 (-251) IL-8R (Ser276Thr) / IL-8R (Met31Arg) IL-8R (Arg335Cys) loci between healthy population and psoriatic population based on the status quo of psoriasis genetics in China and the relevant data of IL-8 and IL-8R in human genome database. By studying the relationship between psoriasis and psoriasis, we found that psoriasis pathogenicity genes or genetic susceptibility genes provide theoretical basis and technical support for gene screening, diagnosis and treatment of psoriasis. Methods: (1) specific primers were designed and synthesized according to genomic database data. (2) 105 Chinese Han patients with PS and 105 healthy controls were randomly selected to extract peripheral venous blood. (3) Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the extracted genomic DNA. (4) genotypes and alleles were calculated by direct gene counting. Genotypes and allelic frequencies were analyzed by Chi-square test (蠂 ~) using SPSS 13.0 software. Results the number of GT genotypes in PS group was higher than that in the control group. The corrected 蠂 ~ 2 test showed that there were significant differences in the composition of GT genotype between PS group and control group (P < 0.05, P < 0.003). The frequency of G allele in PS group was higher than that in control group (P < 0.032), and the frequency of TT genotype of IL-8R (Arg335Cys) was higher than that of control group (P < 0.05). There was significant difference between the genotype and allele frequency of IL-8 (-251) IL-8R (Ser276Thr). There was no statistical significance. Conclusion: (1) IL-8R (Met31Arg) locus may be positively correlated with the susceptibility to psoriasis and may increase the genetic susceptibility of psoriasis. (2) the TT genotype of IL-8R (Arg335Cys) may regulate the expression and function of IL-8R. Therefore, the occurrence of psoriasis was associated with the course of psoriasis. (3) there was no correlation between the genotype and allele of IL-8 (-251) IL-8R (Ser276Thr) locus and the genetic susceptibility of psoriasis.
【學(xué)位授予單位】：四川大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類號(hào)】：D919

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 劉梅;白細(xì)胞介素-8基因多態(tài)性與遲發(fā)性阿爾茨海默病的相關(guān)性研究[D];安徽醫(yī)科大學(xué);2012年

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