本文選題：早期心肌缺血 + 法醫(yī)病理學(xué)診斷��； 參考：《昆明醫(yī)學(xué)院》2006年碩士論文

【摘要】：目的：探討鐵礬蘇木素伊紅染色(Heidenhain stain)對診斷人體急性心肌缺血早期的可能性，最終為法醫(yī)病理學(xué)死因診斷提供詳實的形態(tài)學(xué)依據(jù)，為法醫(yī)病理學(xué)司法鑒定提供更為適用的檢測方法和較為客觀的診斷指標(biāo)。方法：對1995年1月-2005年12月間經(jīng)昆明醫(yī)學(xué)院司法鑒定中心解剖確診為冠心病的48例人體心肌組織，應(yīng)用Heidenhain染色方法檢測急性心肌缺血光鏡下心肌形態(tài)學(xué)改變及其與心肌缺血時間的相關(guān)規(guī)律，并將之與比較成熟的診斷早期心肌缺血的蘇木素堿性復(fù)紅苦味酸染色法(HBFP)及常規(guī)蘇木素伊紅染色法(HE)進行比較。所有數(shù)據(jù)運用SPSS11.0統(tǒng)計分析軟件包做秩和檢驗。結(jié)果：1、HE染色法可以檢驗缺血7h之后的心肌改變；HBFP和Heidenhain染色法可以準(zhǔn)確地檢驗缺血2h后的心肌改變，且心肌缺血的范圍隨著缺血時間的延長而逐漸增寬；Heidenhain染色缺血區(qū)域與HBFP染色缺血區(qū)域、HE染色嗜酸性增強、波狀纖維、收縮帶的出現(xiàn)區(qū)域分布相一致。2、HBFP染色和HE染色在心肌缺血2-7h對診斷心肌早期缺血的差異有統(tǒng)計學(xué)意義，HBFP染色優(yōu)于HE染色；Heidenhain染色和HE染色在心肌缺血2-7h對診斷心肌早期缺血的差異有統(tǒng)計學(xué)意義，，Heidenhain染色優(yōu)于HE染色；Heidenhain染色和HBFP染色在5-6h對診斷心肌早期缺血的差異有統(tǒng)計學(xué)意義，Heidenhain染色優(yōu)于HBFP染色。結(jié)論：Heidenhain染色方法可顯示早期缺血心肌纖維的相關(guān)改變，效果優(yōu)于HE染色方法和HBFP染色方法，加之該法特異性強，穩(wěn)定性好，價格低廉，操作簡便易行，因此推薦將其作為診斷早期心肌缺血的一個首選染色方法加以推廣應(yīng)用。
[Abstract]:Objective: to explore the possibility of early diagnosis of acute myocardial ischemia by using hematoxylin red staining in human body, and to provide a detailed morphological basis for the diagnosis of pathophysiological causes of death. To provide a more suitable method and objective diagnostic index for forensic pathological forensic identification. Methods: from January 1995 to December 2005, 48 cases of coronary heart disease (CHD) were dissected and confirmed by the Center of Forensic expertise of Kunming Medical College. The morphological changes of acute myocardial ischemia and its correlation with myocardial ischemia time were detected by Heidenhain staining. The results were compared with that of HBFPin basic complex picric acid staining and routine hematoxylin eosin staining in the diagnosis of early myocardial ischemia. All data were tested by SPSS 11.0 statistical analysis software package. Results the myocardial changes after 7 hours of ischemia could be examined by 1: 1HE staining. HBFP and Heidenhain staining could accurately detect the myocardial changes after 2 hours of ischemia, and the extent of myocardial ischemia gradually widened with the prolongation of ischemic time. Heidenhain stained ischemic region and HBFP stained ischemic region eosinophilic and wave-like fibers were enhanced by HE staining. The distribution of systolic zone was consistent with that of HE staining and HBFP staining was better than HE staining in the diagnosis of early myocardial ischemia in 2-7 h after myocardial ischemia. There was significant difference between Heidenhain staining and HE staining in the diagnosis of early myocardial ischemia at 2-7 h after myocardial ischemia. Heidenhain staining was better than HE staining and HBFP staining in diagnosing early myocardial ischemia in 5-6 h. Heidenhain staining was better than HBFP staining in the diagnosis of early myocardial ischemia. Conclusion the ratio Heidenhain staining method is superior to the HE staining method and HBFP staining method in showing the changes of early ischemic myocardial fibers. In addition, the method has the advantages of high specificity, good stability, low price and easy to operate. Therefore, it is recommended that it be used as a preferred staining method for the diagnosis of early myocardial ischemia.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：D919

【引證文獻】

相關(guān)碩士學(xué)位論文 前1條

1 王鳳娟;實驗性心肌損傷大鼠血清心肌肌鈣蛋白Ⅰ變化的比較研究[D];上海體育學(xué)院;2010年

本文編號：2016355