本文選題：遺傳多態(tài)性 + 短串聯(lián)重復(fù)(STR)　； 參考：《山西醫(yī)科大學(xué)》2007年碩士論文

【摘要】： 目的:本文對山西漢族群體Y-STR DYS714基因座的等位基因頻率分布進行調(diào)查,并對該基因座的法醫(yī)學(xué)應(yīng)用價值進行探討。 方法:在GDB數(shù)據(jù)庫中查找該基因座;在重復(fù)序列兩端設(shè)計引物;用PCR方法擴增:電泳分型;對其所有等位基因測序:構(gòu)建等位基因分型標準物;按照ISFG原則命名等位基因。 結(jié)果:本文研究顯示:DYS714基因座核心序列為ttttc,被調(diào)查的山西地區(qū)漢族男性個體核心序列的重復(fù)次數(shù)為10～14,共5個等位基因:10、11、12、13、14。基因頻率分別為0.15、0.1583、0.2167、0.3333、0.1416,基因多樣性為0.7808,個人識別能力(DP)和非父排除率(PE)均為0.7808。20例女性樣本DNA無擴增產(chǎn)物。同一尸體血液,器官組織檢測結(jié)果分型一致。15例兩代家系觀察未見突變。 結(jié)論:新篩選到的DYS714具有較高的遺傳多態(tài)性,在法醫(yī)學(xué)及人類學(xué)方面具有應(yīng)用價值。
[Abstract]:Objective: to investigate the allele frequency distribution of Y-STR DYS714 locus in Shanxi Han population, and to explore the value of forensic application of this locus. Primers were designed at both ends of the repeat sequence, amplified by PCR: electrophoretic typing; all alleles were sequenced; alleles were constructed; Results: according to ISFG principle, the core sequence of WDYS714 gene was ttttc. the number of repeats of core sequence of male Han nationality in Shanxi area was 101.The total number of 5 alleles was 10: 111112C1313140.Results: the alleles were named as alleles according to ISFG principle. The results showed that the core sequence of WDYS714 gene was ttttc. The gene frequencies were 0.1583 (0.2167), 0.3333 (0.1416), and (0.7808), the individual recognition ability (DPDP) and the non-paternal exclusion rate (PEE) were 0.7808.20 female samples, respectively. The results of blood, organ and tissue examination of the same cadaver were consistent. 15 cases of two generations were observed without mutation. Conclusion: the newly selected DYS714 has higher genetic polymorphism and has application value in forensic medicine and anthropology.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：D919

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