發(fā)布時(shí)間：2018-06-08 22:50

  本文選題：遺傳多態(tài)性 + 短串聯(lián)重復(fù)(STR)�。� 參考：《山西醫(yī)科大學(xué)》2007年碩士論文

【摘要】： 目的:本文對(duì)山西漢族群體Y-STR DYS714基因座的等位基因頻率分布進(jìn)行調(diào)查,并對(duì)該基因座的法醫(yī)學(xué)應(yīng)用價(jià)值進(jìn)行探討。 方法:在GDB數(shù)據(jù)庫(kù)中查找該基因座;在重復(fù)序列兩端設(shè)計(jì)引物;用PCR方法擴(kuò)增:電泳分型;對(duì)其所有等位基因測(cè)序:構(gòu)建等位基因分型標(biāo)準(zhǔn)物;按照ISFG原則命名等位基因。 結(jié)果:本文研究顯示:DYS714基因座核心序列為ttttc,被調(diào)查的山西地區(qū)漢族男性個(gè)體核心序列的重復(fù)次數(shù)為10～14,共5個(gè)等位基因:10、11、12、13、14。基因頻率分別為0.15、0.1583、0.2167、0.3333、0.1416,基因多樣性為0.7808,個(gè)人識(shí)別能力(DP)和非父排除率(PE)均為0.7808。20例女性樣本DNA無擴(kuò)增產(chǎn)物。同一尸體血液,器官組織檢測(cè)結(jié)果分型一致。15例兩代家系觀察未見突變。 結(jié)論:新篩選到的DYS714具有較高的遺傳多態(tài)性,在法醫(yī)學(xué)及人類學(xué)方面具有應(yīng)用價(jià)值。
[Abstract]:Objective: to investigate the allele frequency distribution of Y-STR DYS714 locus in Shanxi Han population, and to explore the value of forensic application of this locus. Primers were designed at both ends of the repeat sequence, amplified by PCR: electrophoretic typing; all alleles were sequenced; alleles were constructed; Results: according to ISFG principle, the core sequence of WDYS714 gene was ttttc. the number of repeats of core sequence of male Han nationality in Shanxi area was 101.The total number of 5 alleles was 10: 111112C1313140.Results: the alleles were named as alleles according to ISFG principle. The results showed that the core sequence of WDYS714 gene was ttttc. The gene frequencies were 0.1583 (0.2167), 0.3333 (0.1416), and (0.7808), the individual recognition ability (DPDP) and the non-paternal exclusion rate (PEE) were 0.7808.20 female samples, respectively. The results of blood, organ and tissue examination of the same cadaver were consistent. 15 cases of two generations were observed without mutation. Conclusion: the newly selected DYS714 has higher genetic polymorphism and has application value in forensic medicine and anthropology.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類號(hào)】：D919

【參考文獻(xiàn)】

相關(guān)期刊論文 前5條

1 鄭秀芬,王小婭;Y-染色體及其在法醫(yī)鑒定中的作用[J];中國(guó)法醫(yī)學(xué)雜志;1998年03期

2 趙瑞君，趙仰星，李晉華，張偉華，，梁景青，唐澤忠，尹鐳，李國(guó)錦，殷國(guó)榮;TKM──血液DNA快速提取法[J];山西醫(yī)學(xué)院學(xué)報(bào);1996年01期

3 鄭秀芬,程俊峰,葉健,朱少建;4個(gè)Y-STR基因座的多態(tài)性及其法醫(yī)學(xué)應(yīng)用的研究[J];遺傳學(xué)報(bào);2002年02期

4 黃艷梅;祁英杰;徐基清;朱運(yùn)良;伍新堯;;2個(gè)新Y-STR基因座的序列分析和在廣東漢族群體中的多態(tài)性研究[J];遺傳;2006年11期

5 侯一平,吳謹(jǐn),李英碧,張霽,張思仲,褚嘉佑;Y染色體特異短串聯(lián)重復(fù)序列初步研究[J];中華醫(yī)學(xué)遺傳學(xué)雜志;1999年02期

本文編號(hào)：1997532