本文選題：皮膚創(chuàng)傷 + 免疫組化　； 參考：《吉林大學(xué)》2006年碩士論文

【摘要】：目的:探討IL-1,IL-8及VEGF在小鼠皮膚切創(chuàng)中的表達(dá)及其與損傷時(shí)間的關(guān)系。方法:用免疫組化方法檢測(cè)不同損傷時(shí)間(生前傷5min～9d;死后傷5min~30min)小鼠皮膚切創(chuàng)組織中IL-1,IL-8及VEGF的表達(dá)變化。結(jié)果:①IL-1, IL-8, VEGF除了在上皮細(xì)胞的表達(dá)外,還大量出現(xiàn)在肉芽組織的巨噬細(xì)胞和成纖維細(xì)胞內(nèi),而死后損傷組相對(duì)于正常對(duì)照組上述因子無(wú)異常表達(dá)。②皮膚切創(chuàng)后,在早期可誘導(dǎo)IL-1表達(dá),5h達(dá)第一次高峰,1d下降。③傷后3h的損傷皮膚組織中可見(jiàn)部分的多核粒細(xì)胞表達(dá)IL-8,傷后12h～1d ,大部分浸潤(rùn)的多核粒細(xì)胞及部分單核細(xì)胞為IL-8陽(yáng)性。隨傷后時(shí)間延長(zhǎng), IL-8陽(yáng)性細(xì)胞以單核及成纖維細(xì)胞為主。④VEGF在表皮及皮脂腺呈持續(xù)性陽(yáng)性表達(dá),持續(xù)至傷后12h和3d。結(jié)論:IL-1, IL-8, VEGF可作為推斷皮膚損傷時(shí)間的法醫(yī)病理學(xué)標(biāo)志。
[Abstract]:Aim: to investigate the expression of IL-1 IL-8 and VEGF in mouse skin injury and the relationship between IL-8 and injury time. Methods: the expression of IL-1IL-8 and VEGF were detected by immunohistochemical method in the skin of mice with different injury time (5 min or 9 d before death and 30 min after death). Results in addition to the expression of IL-1, IL-8 and VEGF in epithelial cells, they also appeared in macrophages and fibroblasts of granulation tissue. However, there was no abnormal expression of 2. 2 in postmortem injury group compared with normal control group. At the early stage, the expression of IL-1 could be induced to reach the first peak at 1 day. 3. The expression of IL-8 in the injured skin tissue was decreased 3 h after injury. The expression of IL-8 was found in some of the multinucleated granulocytes, 12h~1d, most of the infiltrated multinucleated granulocytes and some monocytes were IL-8 positive. The expression of IL-8 in epidermis and sebaceous gland continued to be positive in the epidermis and sebaceous gland after injury for 12 hours and 3 days after injury, and the positive cells were mainly monocytes and fibroblasts. Conclusion: 1-IL-1, IL-8 and VEGF can be used as forensic pathological markers for estimating the time of skin injury.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：D919

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本文編號(hào)：1909748