發(fā)布時(shí)間：2018-04-28 09:59

  本文選題：頸動(dòng)脈竇壓力感受器 + 死亡模型��； 參考：《重慶醫(yī)科大學(xué)》2005年博士論文

【摘要】：目的:臨床及法醫(yī)學(xué)檢案中時(shí)常遇到頸部受外界壓力刺激后引起死亡的報(bào)導(dǎo)。這類(lèi)案件有典型的臨床經(jīng)過(guò),而無(wú)形態(tài)學(xué)證據(jù),其死亡機(jī)制不清。本實(shí)驗(yàn)借助機(jī)械性外力打擊頸動(dòng)脈竇壓力感受器建立動(dòng)物死亡的模型,通過(guò)生理學(xué)、形態(tài)學(xué)觀察、血清生物化學(xué)對(duì) ANP、NO、NOS 檢測(cè)及反轉(zhuǎn)錄聚合酶鏈反應(yīng)(Reverse Transcription Polymerase Chain Reaction, RT-PCR)檢測(cè) ANP mRNA NOS mRNA 基因表達(dá)等方法手段,研究其死亡的機(jī)制,尋找診斷死亡的客觀指標(biāo)與標(biāo)準(zhǔn)。 材料、方法和結(jié)果: 1、生理學(xué)研究:實(shí)驗(yàn)借助機(jī)械性暴力打擊兔頸動(dòng)脈竇壓力感受器,分為 3 組,共 36 只,實(shí)驗(yàn)組(n=20)、正常對(duì)照組(n1=8)及損傷對(duì)照組(n2=8),觀察其主要生命體征的變化。25%(5/20)動(dòng)物心率下降,且伴隨血壓下降而死亡;75%(15/20)動(dòng)物心率下降(10 次/分),但血壓輕度升高或維持在正常水平。 2、形態(tài)學(xué)研究:實(shí)驗(yàn)組死亡動(dòng)物經(jīng)過(guò)檢驗(yàn)未發(fā)現(xiàn)器質(zhì)性病變,其死亡均由頸動(dòng)脈竇壓力感受器被打擊后所致。形態(tài)學(xué)觀察發(fā)現(xiàn)頸動(dòng)脈竇外膜損傷表現(xiàn)為灶性片狀出血、神經(jīng)纖維斷裂、神經(jīng)纖維軸漿溶解;心肌表現(xiàn)為急性缺血性損傷改變,右心房 ANP 顆粒明顯減少,甚至消失;無(wú)明顯肺水腫表現(xiàn)。而存活組亦有部分頸動(dòng)脈外膜灶性出血、輕度急性心肌缺血性損傷改變(但程度比死亡組輕),右心房 ANP 顆粒尚存較多。
[Abstract]:Objective: to report death caused by external pressure on neck in clinical and forensic cases. This type of case has a typical clinical history, without morphological evidence, its death mechanism is unclear. In this experiment, the animal death model was established by mechanical external force hitting carotid sinus baroreceptor, and the animal death model was observed by physiological and morphological observation. Serum biochemistry and reverse Transcription Polymerase Chain Reaction, RT-PCR were used to detect the expression of ANP mRNA NOS mRNA gene and to study the mechanism of ANP mRNA NOS mRNA death and to find out the objective indexes and criteria for the diagnosis of death. Materials, methods and results: 1. Physiological study: the experiment used mechanical violence to attack carotid sinus baroreceptor in rabbits, which was divided into 3 groups: experimental group (n = 36), normal control group (n = 8) and injury control group (n = 8). The changes of main vital signs were observed. The heart rate decreased 10 times per minute, but the blood pressure was slightly elevated or maintained at normal level. 2. Morphologic study: the dead animals in the experimental group had not found any organic lesions, but the death was caused by the blow of carotid sinus baroreceptor. Morphological observation showed that the injury of carotid sinus adventitia was characterized by focal lamellar hemorrhage, nerve fiber rupture, axonal dissolution of nerve fiber, acute ischemic injury in myocardium, and decrease or even disappearance of ANP granules in right atrium. No obvious pulmonary edema was found. In the survival group, there were some focal carotid hemorrhage and mild acute myocardial ischemic injury (but the degree was lighter than that in the death group, and the ANP granules of right atrium were still more than those in the dead group).
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2005
【分類(lèi)號(hào)】：R653;D919

【相似文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 李劍波;外力打擊兔頸動(dòng)脈竇壓力感受器引起死亡的動(dòng)物模型的建立及其死亡機(jī)制的研究[D];重慶醫(yī)科大學(xué);2005年

相關(guān)碩士學(xué)位論文 前1條

1 劉洋;重力改變對(duì)心血管系統(tǒng)的影響及其防護(hù)措施的仿真研究[D];第四軍醫(yī)大學(xué);2009年

，

本文編號(hào)：1814791