本文選題：法醫(yī)病理學(xué) + 損傷時間推斷　； 參考：《南華大學(xué)》2006年碩士論文

【摘要】：第一部分 大潮氣量通氣建立兔的量佳VILI實驗動物模型 目的 應(yīng)用大潮氣量機械通氣制作兔的最佳VILI實驗動物模型。 方法 根據(jù)黃金分割法原理，實驗組分三組分別采用三種不同潮氣量68mL／kg、60mL／kg和45mL／kg各持續(xù)通氣1分鐘造成兔的急性肺損傷，取0h，，12h，24h，48h四個時間點進行觀察，統(tǒng)計存活數(shù)觀察兔存活情況，計算各時間點肺濕／干重比反映肺水腫嚴重程度及變化，傳統(tǒng)病理切片觀察各時間點肺組織形態(tài)學(xué)改變。 結(jié)果 1、實驗組A(V_T68mL／kg)、實驗組B(V_T60mL／kg)和實驗組C(V_T45mL／kg)兔48h存活率分別為58.33％(7／12)、91.67％(11／12)和100％(12／12)。2、與正常對照組(4.40±0.02)相比，實驗組A(V_T68mL／kg)、實驗組B(V_T60mL／kg)和實驗組C(V_T45mL／kg)的肺濕／干重比在0小時無明顯變化，比值分別為4.46±0.08、4.48±0.08和4.43±0.12：12小時明顯增高，比值分別為5.27±0.18、4.84±0.12和4.64±0.06：在24小時時達峰值，比值分別為5.50±0.18、5.29±0.12和4.88±0.07；48小時后降低，比值分別為5.00±0.31、4.774±0.21和4.46±0.10。3、組織病理學(xué)切片顯示機械通氣后不同時間肺組織均有不同程度形態(tài)學(xué)改變，實驗組A(V_T68mL／kg)肺組織形態(tài)學(xué)改變非常明顯；實驗組B(V_T60mL／kg)肺組織形態(tài)學(xué)改變明顯；實驗組C(V_T45mL／kg)肺組織形態(tài)學(xué)改變不明顯。 結(jié)論 大潮氣量通氣成功建立兔的最佳VILI實驗動物模型，建立的指標為V_T60mL／kg、RR50次／分、FiO_221％。
[Abstract]:Part one: establishment of VILI Experimental Model of Rabbit by Tidal Vapor ventilation Objective to establish the best animal model of VILI by mechanical ventilation. Methods according to the principle of golden division, the experimental group was divided into three groups, three different tidal volume of 68 mL / kg 60 mL / kg and 45mL/kg continuously ventilated for 1 minute each to cause acute lung injury in rabbits. The rabbits were observed at four time points (0 h, 12 h, 24 h and 48 h), and the survival rate was observed. Lung wet / dry weight ratio at different time points was calculated to reflect the severity and changes of pulmonary edema. Results 1. The 48h survival rates of the experimental group (AVT 68 mL / kg, the experimental group BVT 60 mL / kg) and the experimental group Cviv T 45 mL / kg) were 58.337 / 121.67 / 1112) and 100 / 12 / 12 / 12, respectively, compared with 4.40 鹵0.02 in the normal control group. There was no significant change in the lung wet / dry weight ratios in the experimental group AVT 68mL / kg, the experimental group BVVT 60mL / kg and the experimental group CVT 45mLkg-1 / kg. The ratios were 4.46 鹵0.08 鹵4.48 鹵0.08 and 4.43 鹵0.12: 12, respectively. The ratios were 5.27 鹵0.184.84 鹵0.12 and 4.64 鹵0.06, respectively, and reached the peak value at 24 hours. The ratios were 5.50 鹵0.185.29 鹵0.12 and 4.88 鹵0.0748 hours, respectively. The ratios were 5.00 鹵0.31 鹵4.774 鹵0.21 and 4.46 鹵0.10.3 respectively. Histopathological sections showed that there were different degrees of morphologic changes in lung tissue at different time after mechanical ventilation. The lung histomorphologic changes of the experimental group were not obvious. Conclusion the best animal model of VILI in rabbits was successfully established by spring tide ventilation, and the established index was: v _ T _ (60 mL / kg) RR 50 times / min Fio _ 221 路min ~ (-1) 路min ~ (-1) 路min ~ (-1) 路min ~ (-1) 路min ~ (-1).
【學(xué)位授予單位】：南華大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：D919.1

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