本文選題：低電壓 + 電流損傷��； 參考：《山西醫(yī)科大學(xué)》2007年碩士論文

【摘要】： 目的:(1)建立低電壓大鼠電擊傷模型,借以探討大鼠電擊死機(jī)理。(2)探討生前電擊與死后電擊的皮膚的特殊染色是否有區(qū)別,能否用于生前與死后的鑒定。(3)檢測(cè)生前電擊與死后電擊的C-JUN蛋白的表達(dá),探討能否用于生前電擊與死后電擊的鑒別。 方法:(1)Wistar大鼠60只,雌雄不限,200±10g,隨機(jī)分為3組:預(yù)實(shí)驗(yàn)組10只;實(shí)驗(yàn)組40只;對(duì)照組10只。摸索調(diào)整電擊時(shí)間以及間隔時(shí)間,停止電擊后即刻采用BL-420E+生物機(jī)能實(shí)驗(yàn)系統(tǒng)監(jiān)測(cè)大鼠心電圖變化。(2)Wistar大鼠18只,雌雄不限,200±10g,隨機(jī)分為3組:生前電擊死組6只;死后電擊組6只;非電擊對(duì)照組6只。利用(1)中設(shè)計(jì)的電擊模型進(jìn)行電擊,取電擊處及非電擊處皮膚行蘇木素-伊紅(HE)染色、Mowory(ABPAS)法、Alcian blue-核固紅法染色。(3)Wistar大鼠60只,雌雄不限,200±10g,隨機(jī)分為2組,實(shí)驗(yàn)組及對(duì)照組。其中實(shí)驗(yàn)組又分為電擊傷后1h,2h,4h,8h處死組,死后即刻電擊組,死后15min,30min,1h;對(duì)照組分為生前實(shí)驗(yàn)對(duì)照組,死后實(shí)驗(yàn)對(duì)照組。電擊傷組及死后組電擊0.35s,間隔5s。取全腦,石蠟切片,免疫組化AEC染色。 結(jié)果:(1)20例電擊傷組大鼠電擊前及10例正常對(duì)照組大鼠心電圖均正常,P波向上,QRS波群呈Rs型,T波向上。20例電擊傷組大鼠于電后即刻監(jiān)測(cè)心電圖,均可觀察到S-T段有不同程度的抬高,其中有19例出現(xiàn)明顯心動(dòng)過速。(2)皮膚組織HE染色:生前電擊組與對(duì)照組相比最明顯的改變是真皮層膠原纖維腫脹、均質(zhì)化,損傷中心基底層細(xì)胞核縱向伸長,染色較深;死后電擊組改變類似于生前電擊組。皮膚組織Mowory(ABPAS)法染色:死后電擊組與生前電擊組最大區(qū)別在于網(wǎng)織層膠原纖維及彈性纖維基本保持原有形態(tài)且藍(lán)染,核深染,均質(zhì)化不明顯。正常對(duì)照組皮膚均未見異常表現(xiàn)。皮膚組織Alcian blue-核固紅法染色:生前電擊組真皮乳頭層、網(wǎng)織層膠原纖維及彈性纖維均質(zhì)化且明顯較表皮層淡染,纖維組織的細(xì)胞核紅染散在于網(wǎng)織層內(nèi)。死后電擊組網(wǎng)織層膠原纖維及彈性纖維基本保持原有形態(tài),整個(gè)纖維均藍(lán)染,而纖維細(xì)胞核紅染。正常對(duì)照組皮膚均未見異常表現(xiàn)。(3)電擊傷1h、2h、4h、各組大腦皮層及海馬部位C-JUN蛋白呈強(qiáng)陽性表達(dá),其中以4h陽性信號(hào)最強(qiáng),電擊傷后8h上述器官僅有微弱表達(dá);死后電擊組除死后即刻電擊有微弱表達(dá)外,余均未檢測(cè)到陽性表達(dá);各實(shí)驗(yàn)對(duì)照組及死后電擊各組腦組織基本檢測(cè)不到C-JUN蛋白的表達(dá)。 結(jié)論:(1)該電擊傷模型動(dòng)物具有明顯電流損傷的特點(diǎn),可用于電流損傷的法醫(yī)學(xué)研究。(2)生前電擊與死后電擊的皮膚Alcian blue特殊染色有區(qū)別,可作為鑒別生前電擊與死后電擊的輔助指標(biāo)。(3)生前電擊、死后電擊大鼠腦組織中C-JUN蛋白表達(dá)有區(qū)別,且其表達(dá)與電擊傷后經(jīng)過時(shí)間有一定相關(guān)性,可以作為鑒別生前電擊與死后電擊的一個(gè)指標(biāo)。
[Abstract]:Objective: to establish a low-voltage rat model of electric shock injury in order to explore the mechanism of electrocution in rats. (2) to explore the difference between antemortem electric shock and postmortem shock on the skin.Whether it can be used in the identification of antemortem and postmortem shock, the expression of C-JUN protein in antemortem shock and postmortem shock was detected, and the identification of antemortem shock and postmortem shock was discussed.Methods 60 male and female Wistar rats were randomly divided into 3 groups: experimental group (n = 10), experimental group (n = 40) and control group (n = 10).The electrocardiogram (ECG) of 18 rats were monitored by BL-420E biological function test system immediately after the electric shock was stopped. The rats were randomly divided into 3 groups: antemortem electroshock group (n = 6), postmortem shock group (n = 6) and postmortem shock group (n = 6).There were 6 rats in the control group.The experimental group was divided into two groups: one hour, two hours and four hours and eight hours after the injury, and the control group was divided into two groups: the antemortem experimental group, the postmortem experimental control group, the postmortem experimental control group, the postmortem experimental control group and the postmortem experimental control group.The electric shock time was 0.35 s in the electrocution group and the postmortem group, the interval was 5 s.The whole brain was stained with paraffin section and immunohistochemical AEC staining.Results the electrocardiogram (ECG) of 20 rats in the control group and 10 normal rats in the control group were in the form of Rs / T wave. The electrocardiogram was monitored immediately after electric shock in the 20 cases of electric injury group (n = 20) and the normal control group (n = 10).It was observed that the S-T segment was elevated in varying degrees, including 19 cases with obvious tachycardia.) HE staining of skin tissue was observed. The most obvious changes in the antemortem shock group compared with the control group were the swelling and homogenization of collagen fibers in the dermis.The nuclei of the basal layer of the injured center were elongated longitudinally and stained deeply, and the changes of the postmortem shock group were similar to those of the antemortem shock group.No abnormal skin manifestations were found in the normal control group.After death, the reticulated collagen fibers and elastic fibers remained in their original form, the whole fibers were blue stained, and the nuclei of the fibers were red stained.In the normal control group, there was no abnormal appearance in the skin. No abnormal expression was found in the skin of the control group. The C-JUN protein was strongly expressed in the cortex and hippocampus of all groups at 1 h and 2 h and 4 h after the injury. The strongest positive signal was found in the cortex and hippocampus at 4 h, and the weak expression was observed in the above organs at 8 h after electrical injury.In the postmortem shock group, the expression of C-JUN protein was not detected except the weak expression in the immediate postmortem shock group, and the expression of C-JUN protein was not detected in the brain tissues of the experimental control group and the postmortem shock group.Conclusion (1) the model animal model of electric shock has obvious characteristics of electric current injury. It can be used in forensic research of electric current injury. (2) there is a difference between antemortem electric shock and postmortem electric shock in skin Alcian blue special staining.The expression of C-JUN protein in the brain tissue of postmortem shock rats was different, and the expression of C-JUN protein was correlated with the time elapsed after electric shock injury, which could be used as an auxiliary index for distinguishing antemortem electric shock from postmortem shock.It can be used as an indicator to distinguish antemortem shock from postmortem shock.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類號(hào)】：D919

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 盧德章;范宏剛;于世明;張欒松;馬昆;姜?jiǎng)?譚麗娟;王洪斌;;替來他明誘導(dǎo)大鼠中樞JUN核蛋白表達(dá)的研究[J];中國獸醫(yī)學(xué)報(bào);2012年11期

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本文編號(hào)：1736221