人指紋的STR分型檢測及法醫(yī)學(xué)應(yīng)用研究
發(fā)布時間:2018-02-25 18:07
本文關(guān)鍵詞: 汗?jié)撝讣y 血潛指紋 低拷貝模板(LCN) 指紋顯現(xiàn) 磁珠法 硅珠法 microcon-100 聚合酶鏈反應(yīng) 出處:《昆明醫(yī)學(xué)院》2006年碩士論文 論文類型:學(xué)位論文
【摘要】:目的 建立滲透性與非滲透性載體上顯現(xiàn)與未顯現(xiàn)的汗?jié)撝讣y、血潛指紋的DNA檢驗方法,最大限度地發(fā)揮指紋特別是殘缺不全以及模糊不清指紋的證據(jù)作用,為偵查破案及訴訟提供科學(xué)依據(jù)。方法采用Chelex-100法與Microcon-100純化濃縮相結(jié)合提取法、磁珠法和Invisorb spin forensic試劑盒提取純化人指紋中DNA,低拷貝模板(LCN)STR復(fù)合擴(kuò)增、熒光電泳檢測。結(jié)果首次建立了用Invisorb spin forensic試劑盒及QIAGENE系統(tǒng)對汗?jié)撝讣yDNA的提取純化方法。玻片、紙張、膠帶紙上的汗?jié)撝讣y均可得到完整的power plex16基因座的STR分型。對各種載體上經(jīng)過常用的指紋顯現(xiàn)法顯現(xiàn)的汗?jié)撝讣y進(jìn)行了系統(tǒng)研究。結(jié)果表明用銅粉、鋁粉、熒光粉、黑磁粉、“502”膠、茚三酮、磺酸雙三嗪熒光顯色液顯現(xiàn)的玻片和紙張上汗?jié)撝讣y可成功進(jìn)行STR分型,而硝酸銀顯現(xiàn)的指紋不能成功進(jìn)行STR基因分型,另外四甲基聯(lián)苯胺、茚三酮、鋁粉和銅粉顯現(xiàn)后1小時、2周及1月提取的血潛指紋DNA,STR基因分型結(jié)果無明顯影響,,而氨基黑10B對DNA分型有一定影響。結(jié)論 建立了汗?jié)撝讣y、血潛指紋顯現(xiàn)前后的微量DNA檢驗方法,并成功應(yīng)用于實際案例。課題研究所創(chuàng)建的方法簡單新穎、結(jié)果靈敏、穩(wěn)定、可重復(fù)性好。
[Abstract]:Objective to establish a DNA method for detecting perspiration latent fingerprints and blood latent fingerprints on permeable and non-permeable carriers, so as to give full play to the evidence function of fingerprints, especially incomplete fingerprints and fuzzy fingerprints. Methods Chelex-100 method combined with Microcon-100 purification and concentration extraction method, magnetic bead method and Invisorb spin forensic kit were used to extract and purify DNA from human fingerprint. Results the method of extracting and purifying sweat latent fingerprint DNA by Invisorb spin forensic kit and QIAGENE system was established for the first time. The sweat latent fingerprint on the tape paper can obtain the complete STR typing of the power plex16 locus. The sweat latent fingerprint displayed by the common fingerprint display method on various carriers was studied systematically. The results showed that copper powder, aluminum powder and phosphor were used. Black magnetic powder, "502" gel, indene trione, ditriazine sulfonic acid fluorescent chromogenic liquid glass slide and paper sweat latent fingerprint can be successfully classified by STR, but silver nitrate fingerprint can not successfully carry out STR genotyping, and tetramethyl benzidine, indene trione, The results of DNA STR genotyping from blood latent fingerprints extracted from aluminum powder and copper powder at 1 hour and 2 weeks after appearance and on January had no significant effect, but amino-black 10B had some influence on DNA typing. Conclusion the sweating latent fingerprint was established. The trace DNA method before and after the development of blood latent fingerprints was successfully applied to practical cases. The method established in this paper is simple and novel, the results are sensitive, stable and reproducible.
【學(xué)位授予單位】:昆明醫(yī)學(xué)院
【學(xué)位級別】:碩士
【學(xué)位授予年份】:2006
【分類號】:D919
【引證文獻(xiàn)】
相關(guān)碩士學(xué)位論文 前1條
1 俞麗娟;激光顯微捕獲切割技術(shù)在脫落細(xì)胞類檢材中的方法學(xué)建立及其法醫(yī)學(xué)應(yīng)用研究[D];山西醫(yī)科大學(xué);2012年
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