本文關(guān)鍵詞： p53 caspase-8 哮喘 藥物副作用　出處：《四川大學(xué)》2005年碩士論文　論文類型：學(xué)位論文

【摘要】：研究背景及目的: 支氣管哮喘病理生理學(xué)研究發(fā)現(xiàn)支氣管哮喘易于并發(fā)心肌損害。作者曾對(duì)12例支氣管哮喘猝死病例進(jìn)行病例形態(tài)學(xué)研究,發(fā)現(xiàn)支氣管哮喘除肺部病變外,還并發(fā)心肌細(xì)胞萎縮、壞死和瘢痕形成。支氣管哮喘患者心肌損害的機(jī)理尚不清楚。另外有學(xué)者報(bào)道支氣管哮喘常用治療藥腎上腺素可能促進(jìn)心肌細(xì)胞凋亡。本實(shí)驗(yàn)?zāi)康氖峭ㄟ^(guò)哮喘模型的建立以及應(yīng)用不同的藥物治療來(lái)分析心肌細(xì)胞損害的機(jī)理。 方法: 健康SD雄性大鼠120只,隨機(jī)分為正常對(duì)照組、實(shí)驗(yàn)對(duì)照組、不治療組、腎上腺素治療組、氨茶堿治療組以及腎上腺素氨茶堿聯(lián)合治療組六個(gè)實(shí)驗(yàn)組,每組又分為四小組(2W、4W、6W、8W),每小組5只大鼠。于實(shí)驗(yàn)第2W、4W、6W、8W分別處死大鼠。應(yīng)用免疫組化技術(shù)對(duì)凋亡促進(jìn)因子p53、凋亡啟動(dòng)因子caspase-8表達(dá)進(jìn)行研究,用IMAGE圖像分析系統(tǒng)對(duì)陽(yáng)性結(jié)果進(jìn)行半定量測(cè)定。 結(jié)果: (1)正常對(duì)照組及實(shí)驗(yàn)對(duì)照組SD雄性大鼠p53、caspase-8呈陰性表達(dá),隨實(shí)驗(yàn)時(shí)間的延長(zhǎng),表達(dá)強(qiáng)度無(wú)表化;(2)各實(shí)驗(yàn)組心肌細(xì)胞p53、caspase-8陽(yáng)性表達(dá)。表達(dá)面積及面積灰度積在第2周較對(duì)照組高(p0.01),隨著時(shí)間的延長(zhǎng)陽(yáng)性信號(hào)表達(dá)面積及面積灰度積逐漸上升,第8周,達(dá)高峰。(3)
[Abstract]:Research background and purpose:
Bronchial asthma pathophysiology of bronchial asthma with myocardial damage. The author has easy to sudden death cases in 12 cases of bronchial asthma were morphologically studied were found in bronchial asthma lung lesions, but also complicated with myocardial cell atrophy, necrosis and scar formation. A mechanism of myocardial damage in patients with bronchial asthma is unclear. In addition, some scholars have reported that bronchial the commonly used drugs for the treatment of asthma adrenaline may promote the apoptosis of myocardial cells. The purpose of this experiment is to establish the mechanism through the asthma model and drug treated by analysis of myocardial cell injury.
Method:
Healthy male SD 120 rats were randomly divided into normal control group, experimental control group, no treatment group, epinephrine treatment group, aminophylline treatment group and epinephrine aminophylline treatment group and six experimental groups, each group was divided into four groups (2W, 4W, 6W, 8W), 5 rats per group. In the experiment of 2W, 4W, 6W, 8W respectively. The rats were sacrificed by immunohistochemistry on the apoptosis promoting factor p53, apoptosis factor caspase-8 expression study system for semi quantitative positive results of IMAGE was determined by image analysis.
Result:
(1) normal control group SD male rats and experimental group p53, the expression of caspase-8 was negative, with the time of the experiment, the expression intensity of tableless; (2) the myocardial cells of the experimental group p53, Caspase-8 positive expression. The expression product of gray area and area is higher than the control group in second weeks (P0.01). With the extension of time the positive signal expression area and gray area area gradually increased, eighth weeks, and reached the peak (3).

【學(xué)位授予單位】：四川大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R562.25;D919

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