喀斯特地區(qū)土壤中鈣含量較高。土壤鈣含量過高會抑制植物生長,進(jìn)而影響農(nóng)作物產(chǎn)量和質(zhì)量。目前,對植物應(yīng)答和耐受高鈣脅迫的機(jī)理研究還不深入。miRNAs在植物對生物和非生物脅迫的響應(yīng)中起重要調(diào)控作用。本文應(yīng)用轉(zhuǎn)錄組測序,對高鈣響應(yīng)擬南芥根部miRNAs進(jìn)行分析,篩選到受高鈣脅迫顯著影響的12個miRNAs,并對其靶基因進(jìn)行了預(yù)測和分析。實(shí)時定量PCR分析證實(shí),大部分差異表達(dá)miRNAs和其靶基因表達(dá)負(fù)相關(guān)。本研究為進(jìn)一步揭示miRNAs在植物響應(yīng)高鈣脅迫過程中的調(diào)控作用奠定了基礎(chǔ)。 

【文章來源】：生命科學(xué)研究. 2020,24(01)CSCD

【文章頁數(shù)】：8 頁

【文章目錄】：
1 Materials and methods
    1.1 Plant growth and high calcium treatment
    1.2 Total RNA extraction and small RNA iso-lation and sequencing
    1.3 Bioinformatics analysis of miRNA sequen-cing data
    1.4 Differential expression analysis of miRNAs
    1.5 Prediction and annotation of target genes for differentially expressed miRNAs
    1.6 RT-qPCR analysis of high calcium-res-ponsive miRNAs and their predicted target genes
2 Results
    2.1 High-throughput sequencing results of sm-all RNA libraries from Arabidopsis roots
    2.2 Differential expression analysis of Ara-bidopsis miRNAs under high calcium stress
    2.3 Target prediction and annotation of differ-entially expressed miRNAs
    2.4 RT-qPCR analysis of high calcium-re-sponsive miRNAs and their target genes
3 Discussion
Supplement data



本文編號：3370242