千里光屬（Senecio）植物除南極洲外,在世界其他地區(qū)均有分布。該屬植物多具有重要經(jīng)濟(jì)價(jià)值,包括具觀賞價(jià)值的多肉品種,及具藥用價(jià)值的物種。在肯尼亞,千里光屬植物主要分布在山區(qū),肯尼亞山地區(qū)的該屬植物因物種多樣性高、生活史復(fù)雜程度高,是研究千里光屬植物分類及系統(tǒng)進(jìn)化的良好材料。本研究選擇肯尼亞山區(qū)五種千里光屬植物S.keniophytum、S.schweinfurthii、S.roseiflorus、S.moorei、&purtschellerS.采用高通量測序技術(shù)對(duì)五種千里光屬植物的葉綠體基因組進(jìn)行測序,使用在線程序Dual Organellar Genome Annotator進(jìn)行葉綠體基因組注釋,Mauve軟件進(jìn)行序列比對(duì),植物RNA編輯器用于RNA編輯,MIcroSAtellite用于識(shí)別微衛(wèi)星RAxML用于微衛(wèi)星和系統(tǒng)發(fā)育分析的。結(jié)果如下:（1）五種千里光屬植物拼接組裝后的葉綠體基因組大小從151,191bp（S.purtschelleri）到151,413bp（S.keniophytum）,葉綠體基因組包含87個(gè)蛋白質(zhì)編碼基因,8個(gè)rRNA基因和34個(gè)tRNA基因,... 

【文章來源】：中國科學(xué)院大學(xué)(中國科學(xué)院武漢植物園)湖北省

【文章頁數(shù)】：78 頁

【學(xué)位級(jí)別】：碩士

【文章目錄】：
摘要
Abstract
Chapter 1 Introduction
    1.1 Background information
    1.2 Uses of Senecio species
    1.3 Statement of the problem
    1.4 Justification
    1.5 Objectives
    1.6 Literature review
        1.6.1 Taxonomy and distribution
        1.6.2 Hybridization among the Senecio species
        1.6.3 Phylogenetic studies among the Senecio species
Chapter 2 Materials and Methods
    2.1 Study site
    2.2 Sample collection DNA extraction and sequencing
    2.3 DNA extraction and sequencing
    2.4 Genome annotation
    2.5 Genome alignments and IR expansions and contractions
    2.6 RNA editing and codon usage
    2.7 Microsatellites analysis
    2.8 Phylogenetic analysis and sequence divergence hotspots
Chapter 3 Results
    3.1 Genome structure, size, gene content and organization in Senecio
    3.2 Genomic functional characterization
    3.3 Whole-genome comparative analysis and IR expansions and contractions
    3.4 RNA editing and Codon usage
    3.5 Microsatellites formation
    3.6 Phylogenetic relationship and sequence divergence hotspots
Chapter 4 Discussion
Chapter 5 Conclusion and Future Prospects
References
Additional files
Acknowledgements
Author profile



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